biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 61:622-630, 2017 | DOI: 10.1007/s10535-017-0730-y

Effect of salicylic acid on freezing injury in peach floral organs and the expressions of CBF genes

B. B. Zhang1, L. Guo1, Z. Z. Song1, M. L. Yu1, R. J. Ma1,*
1 Institute of Horticulture, Jiangsu Academy of Agricultural Sciences and Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, Nanjing, P.R. China

We used flowering branches at the budding stage of two peach cultivars Xiahui 6 and Xiacui with different cold resistance to explore the effect of exogenous salicylic acid (SA) on the freezing injury of peach floral organs and the molecular mechanism. Using water application as the negative control, the effects of spraying with SA at concentrations of 20 or 100 mg dm-3 on stigma receptivity, frost damage characteristics of floral organs, and the expressions of C-repeat-binding factor (PpCBF) gene family members were investigated at 0 °C. No significant frost damage was observed on petals in all treatments. No frost damage was seen in the ovary and style under 20 mg dm-3 SA treatment, but damage was substantial at the other two treatments. Cultivar Xiahui 6 was more susceptible to freezing than cv. Xiacui. The expression peaks of PpCBFs in the SA-pretreated floral organs occurred at 3 or 6 h after low temperature treatment, and peak time was closely related to peach cultivar, organ, and SA concentration. This indicates that appropriate concentration of exogenous SA may alleviate freezing damage to floral organs and enhance cold resistance by the regulated expression pattern of PpCBF.

Keywords: ovary; petals; Prunus persica; stigma; style
Subjects: salicylic acid; freezing stress; floral organs; ovary; petals; stigma; style; CBF genes; peach

Received: November 2, 2016; Revised: January 10, 2017; Accepted: January 19, 2017; Published: December 1, 2017  Show citation

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Zhang, B.B., Guo, L., Song, Z.Z., Yu, M.L., & Ma, R.J. (2017). Effect of salicylic acid on freezing injury in peach floral organs and the expressions of CBF genes. Biologia plantarum, 61(4), 622-630. doi: 10.1007/s10535-017-0730-y
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    References

    1. Alaey, M., Babalar, M., Naderi, R., Kafi, M.: Effect of pre- and post-harvest salicylic acid treatment on physio-chemical attributes in relation to vase-life of rose cut flowers. - Postharvest Biol. Tech. 61: 91-94, 2011. Go to original source...
    2. Artlip, T.S., Wisniewski, M.E., Bassett, C.L., Norelli, J.L.: CBF gene expression in peach leaf and bark tissues is gated by a circadian clock. - Tree Physiol. 33: 866-877, 2013. Go to original source...
    3. Burke, M.J., Gusta, L.V., Quamme, H.A., Weiser, C.J., Li, P.H.: Freezing and injury in plants. - Annu. Rev. Plant Physiol. 27: 507-528, 1976. Go to original source...
    4. Carpenter, J.F., Hand, S.C., Crowe, L.M., Crowe, J.H.: Cryoprotection of phosphofructokinase with organic solutes: characterization of enhanced protection in the presence of divalent cations. - Arch. Biochem. Biophys. 250: 505-512, 1986. Go to original source...
    5. Chang, S., Puryear, J., Cairney, J.: A simple and efficient method for isolating RNA from pine trees. - Plant mol. Biol. Rep. 11: 113-116, 1993. Go to original source...
    6. Chinnusamy, V., Zhu, J., Zhu, J.K.: Gene regulation during cold acclimation in plants. - Physiol. Plant. 126: 52-61, 2006. Go to original source...
    7. Chinnusamy, V., Zhu, J., Zhu, J.K.: Cold stress regulation of gene expression in plants. - Trends Plant Sci. 12: 444-451, 2007. Go to original source...
    8. Chu, J.Z., Yao, X.Q., Zhang, Z.N.: Responses of wheat seedlings to exogenous selenium supply under cold stress. - Biol. Trace Element Res. 136: 355-363, 2010. Go to original source...
    9. Dafni, A.: Pollination Ecology. - Oxford University Press, New York 1992.
    10. Fowler, S., Thomashow, M.F.: Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. - Plant Cell 14: 1675-1690, 2002. Go to original source...
    11. Guo, Q., Meng, L., Mao, P.C., Jia, Y.Q., Shi, Y.J.: Role of exogenous salicylic acid in alleviating cadmium-induced toxicity in Kentucky bluegrass. - Biochem. Syst. Ecol. 50: 269-276, 2013. Go to original source...
    12. Huang, M., Guo, Z.: Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity. - Biol. Plant. 49: 81-84, 2005. Go to original source...
    13. Hufkens, K., Friedl, M.A., Keenan, T.F., Sonnentag, O., Bailey, A., O'Keefe, J., Richardson, A.D.: Ecological impacts of a widespread frost event following early spring leaf-out. - Global Change Biol. 18: 2365-2377, 2012. Go to original source...
    14. Jaglo-Ottosen, K.R., Kleff, S., Amundsen, K.L., Zhang, X., Haake, V., Zhang, J.Z., Deits, T., Thomashow, M.F.: Components of the Arabidopsis C-repeat/dehydrationresponsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species. - Plant Physiol. 127: 910-917, 2001. Go to original source...
    15. Janda, T., Szalai, G., Rios-Gonzalez, K., Veisz, O., Páldi, E.: Comparative study of frost tolerance and antioxidant activity in cereals. - Plant Sci. 164: 301-306, 2003. Go to original source...
    16. Kachroo, P., Venugopal, S.C., Navarre, D.A., Lapchyk, L., Kachroo, A. Role of salicylic acid and fatty acid desaturation pathways in ssi2-mediated signaling. - Plant Physiol. 139: 1717-1735, 2005. Go to original source...
    17. Kang, H.M., Saltveit, M.E.: Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid. - Physiol. Plant. 115: 571-576, 2002. Go to original source...
    18. Karimi, M., Ebadi, A., Mousavi, S.A., Salami, S.A., Zarei, A. Comparison of CBF1, CBF2, CBF3 and CBF4 expression in some grapevine cultivars and species under cold stress. - Sci. Hort. 197: 521-526, 2015. Go to original source...
    19. Karlidag, H., Yildirim, E., Turan, M.: Salicylic acid ameliorates the adverse effect of salt stress on strawberry. - Sci. Agr. 66: 180-187, 2009. Go to original source...
    20. Liang, L., Zhang, B., Yin, X.R., Xu, C.J., Sun, C.D., Chen, K.S.: Differential expression of the CBF gene family during postharvest cold storage and subsequent shelf-life of peach fruit. - Plant mol. Biol. Rep. 31: 1358-1367, 2013. Go to original source...
    21. Liu, H., Ouyang, B., Zhang, J.H., Wang, T.T., Li, H.X., Zhang, Y.Y., Yu, C.Y., Ye, Z.B.: Differential modulation of photosynthesis, signaling, and transcriptional regulation between tolerant and sensitive tomato genotypes under cold stress. - Plos ONE 7: e50785, 2012. Go to original source...
    22. Liu, J.Y., Zhu, Y., Huang, L.L., Xu, X., Li, G.Z., Hao, L.: Combined effect of ethylene- and salicylic acid-signaling insensitive mutation on Arabidopsis response to low temperature. - Biol. Plant. 60: 523-531, 2016. Go to original source...
    23. Livak, K.J., Schmittgen, T.D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. - Methods 25: 402-408, 2001. Go to original source...
    24. Luo, Y.L., Su, Z.L., Bi, T.J., Cui, X.L., Lan, Q.Y.: Salicylic acid improves chilling tolerance by affecting antioxidant enzymes and osmoregulators in sacha inchi (Plukenetia volubilis). - Braz. J. Bot. 37: 357-363, 2014. Go to original source...
    25. Luo, Z.S., Chen, C., Xie, J.: Effect of salicylic acid treatment on alleviating postharvest chilling injury of Qingnai plum fruit. - Postharvest Biol. Tech. 62: 115-120, 2011. Go to original source...
    26. Marandi, R.J., Hassani, A., Abdollahi, A., Hanafi, S.: Application of Carum copticum and Satureja hortensis essential oils and salicylic acid and silver thiosulfate in increasing the vase life of cut rose flower. - J. Med. Plant Res. 5: 5034-5038, 2011. Go to original source...
    27. Métraux, J.P., Signer, H., Ryals, J., Ward, E., Wyssbenz, M., Gaudin, J., Raschdorf, K., Schmid, E., Blum, W., Inverardi, B.: Increase in salicylic acid at the onset of systemic acquired resistance in cucumber. - Science 250: 1004-1006, 1990. Go to original source...
    28. Miura, K., Ohta, M.: SIZ1, a small ubiquitin-related modifier ligase, controls cold signaling through regulation of salicylic acid accumulation. - J. Plant Physiol. 167: 555-560, 2010. Go to original source...
    29. Promyou, S., Ketsa, S., Doorn, W.G.V.: Salicylic acid alleviates chilling injury in anthurium (Anthurium andraeanum L.) flowers. - Postharvest Biol. Tech. 64: 104-110, 2012. Go to original source...
    30. Senaratna, T., Touchell, D., Bunn, E., Dixon, K.: Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. - Plant Growth Regul. 30: 157-161, 2000. Go to original source...
    31. Takuhara, Y., Kobayashi, M., Suzuki, S.: Low-temperatureinduced transcription factors in grapevine enhance cold tolerance in transgenic Arabidopsis plants. - J. Plant Physiol. 168: 967-975, 2011. Go to original source...
    32. Thomashow, M.F., Gilmour, S.J., Stockinger, E.J., Jaglo-Ottosen, K.R., Zarka, D.G.: Role of the Arabidopsis CBF transcriptional activators in cold acclimation. - Physiol. Plant. 112: 171-175, 2001. Go to original source...
    33. Uemura, M., Steponkus, P. L.: A contrast of the plasma membrane lipid composition of oat and rye leaves in relation to freezing tolerance. - Plant Physiol. 104: 479-496, 1994. Go to original source...
    34. Van Buskirk, H.A., Thomashow, M.F.: Arabidopsis transcription factors regulating cold acclimation. - Physiol. Plant. 126: 72-80, 2006. Go to original source...
    35. Wang, L., Chen, S., Kong, W., Li, S., Archbold, D.D.: Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during cold storage. - Postharvest Biol. Tech. 41: 244-251, 2006. Go to original source...
    36. Wanner, L.A., Junttila, O.: Cold-induced freezing tolerance in Arabidopsis. - Plant Physiol. 120: 391-400, 1999. Go to original source...
    37. Ward, E.R., Uknes, S.J., Williams, S.C., Dincher, S.S., Wiederhold, D.L., Alexander, D.C., Ahlgoy, P., Metraux, J.P., Ryals, J.A.: Coordinate gene activity in response to agents that induce systemic acquired resistance. - Plant Cell 3: 1085-1094, 1991. Go to original source...
    38. Wisniewski, M., Norelli, J., Bassett, C., Artlip, T., Macarisin, D.: Ectopic expression of a novel peach (Prunus persica) CBF transcription factor in apple (Malus × domestica) results in short-day induced dormancy and increased cold hardiness. - Planta 233: 971-983, 2011. Go to original source...
    39. Yuasa, T., Nakamura, J., Ishibashi, Y., Iwayainoue, M.: Tomato inducer of CBF expression 1 (SIICE1) is involved in cold and salt stress signaling. - Amer. J. exp. Agr. 4: 785-796, 2014. Go to original source...
    40. Zhang, J.H., Wang, Y.Z., Sun, H.Y., Yang, L., Jiang, F.C.: Effects of exogenous salicylic acid on antioxidant enzymes and CBF transcription factor in apricot (Prunus armeniaca L.) flowers under chilling stress. - Plant Physiol. J. 50: 171-177, 2014.
    41. Zhang, X., Fowler, S.G., Cheng, H., Lou, Y., Rhee, S.Y., Stockinger, E.J., Thomashow, M.F.: Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant Arabidopsis. - Plant J. 39: 905-919, 2004. Go to original source...
    42. Zhao, D.Y., Lin, S., Bei, F., Yu, M.M., Yang, Z., Lv, S.N., Sheng, J.P.: Ethylene and cold participate in the regulation of LeCBF1 gene expression in postharvest tomato fruits. - FEBS Lett. 583: 3329-3334, 2009. Go to original source...

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