biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 61:717-725, 2017 | DOI: 10.1007/s10535-017-0733-8

The cytotoxic targets of anatase or rutile + anatase nanoparticles depend on the plant species

S. Silva1,2,*, H. Oliveira2,3, A. M. S. Silva1, C. Santos4
1 Department of Chemistry, QOPNA, University of Aveiro, Aveiro, Portugal
2 CESAM, University of Aveiro, Aveiro, Portugal
3 Department of Biology, University of Aveiro, Aveiro, Portugal
4 Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal

The potential toxicity of nanoparticles (NPs) is under debate. Information about TiO2 NPs phytotoxicity is still limited partly due to the different TiO2 NP forms that may be found in the environment. The present work investigated the impact of different TiO2 NPs forms (rutile and anatase) on germination, growth, cell cycle profile, ploidy level, and micronucleus formation in Lactuca sativa (lettuce) and Ocimum basilicum (basil). Seeds were exposed to anatase (ana) or rutile + anatase (rut+ana) at concentrations 5 - 150 mg dm-3 for 5 d and after that different parameters were analyzed. Rut+ana showed high potential to impair germination and growth. On the other hand, ana alone showed a positive influence on seedling growth. Despite that, ana induced severe alterations in cell cycle dynamics. Regarding species, basil was more sensitive to TiO2 NPs cytostatic effects (delay/arrest in G0/G1 phase), whereas in lettuce, TiO2 NPs were more genotoxic (micronucleus formation increase). Finally, we propose that, besides germination and plant growth, cell cycle dynamics and micronucleus formation can be sensitive biomarkers of these NPs.

Keywords: abiotic stress; cell cycle; genotoxicity; micronucleus; phytotoxicity; titanium dioxide
Subjects: nanoparticles; atanase; rutile; cell cycle; genotoxicity; germination; growth; lettuce; sweet basil

Received: August 23, 2016; Revised: January 26, 2017; Accepted: January 27, 2017; Published: December 1, 2017  Show citation

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Silva, S., Oliveira, H., Silva, A.M.S., & Santos, C. (2017). The cytotoxic targets of anatase or rutile + anatase nanoparticles depend on the plant species. Biologia plantarum, 61(4), 717-725. doi: 10.1007/s10535-017-0733-8
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