High-Mobility Group Chromatin Proteins 1 and 2 Functionally Interact with Steroid Hormone Receptors To Enhance Their DNA Binding In Vitro and Transcriptional Activity in Mammalian Cells

Expression of recombinant receptors and HMG-1 in the baculovirus system.

Recombinant baculovirus transfer vectors for human PR-A and PR-B were constructed by inserting the cDNAs for each receptor isoform into BamHI sites of pBlueBacHis-2 (InvitroGen, San Diego, Calif.). This placed PR coding sequences in frame with amino-terminal plasmid sequences that contain an ATG translation start site, six sequential histidine residues, and an enterokinase cleavage site. A polyhistidine-tagged human GR baculovirus transfer vector was constructed by inserting a BamHI-XhoI fragment from pI9 (18) encoding amino acids 9 to 777 of human GR in frame into pBlueBacHis-2 (C) restricted with BamHI and HindIII. The XhoI and HindIII ends of GR cDNA were blunted by filling in before joining. The pI9 plasmid was provided by Ron Evans (Salk Institute, San Diego, Calif.). A 2.8-kb full-length human androgen receptor (hAR) cDNA, plus sequences coding for one extra amino-terminal alanine and six histidine residues, was cloned into a 9.1-kb pAcC4 baculovirus transfer vector (Cetus Corp.) that resulted in the following amino-terminal sequence: Met-Ala-His₆-Glu-Val. The hAR recombinant baculovirus transfer vector was constructed by PCR amplification with a primer that placed an NcoI site at the starting methionine of human AR, making it coincident with the position of the starting methionine of the polyhedron protein. A triple-ligation reaction was performed as previously described (71). A recombinant baculovirus transfer vector for HMG-1 was constructed by inserting rat HMG cDNA from pBS-rHMG-1 into EcoRI and SalI sites of pBlueBacHis-2(B). The rat HMG coding region was placed in frame with amino-terminal sequences of the plasmid containing an ATG translation start site, six sequential histidine residues, and an enterokinase cleavage site. A recombinant baculovirus transfer vector for steroid receptor coactivator 1 (SRC-1) (43) was constructed by inserting SRC-1 cDNA (amino acids 1 to 1140) from pBK-CMVSRC-1 (provided by Bert O’Malley, Ming-Jer Tsai, and Sergio Oñate, Baylor College of Medicine) into BamHI and PstI sites of the baculovirus transfer plasmid, pBlueBacHis-2(C). The SRC-1 coding region was also inserted in frame with amino-terminal plasmid sequences containing an ATG translation start site, six sequential histidine residues, and an enterokinase cleavage site. Cloning junctures in all transfer plasmids were sequenced (Sequenase 2.0; U.S. Biochemicals), and inserts were determined to be correctly oriented and in frame with the starting ATG codon and the six sequential histidine residues.

To construct recombinant viruses encoding the above proteins, Spodoptera frugiperda insect cells (Sf9) were cotransfected with baculovirus transfer plasmid and wild-type Autographa californica nuclear polyhedrosis virus baculovirus DNA as previously described (10). Recombinant viruses were identified by visual inspection under a reversed-phase light microscope, and the individual viruses were plaque purified. Viruses were screened by Western blot analysis of infected Sf9 cells for their ability to express protein. PR, AR, and GR proteins were produced in Sf9 cells in a stirred oxygenated 5-liter bioreactor (Applikon, Inc.). Cells were grown in Grace’s insect medium (Gibco-BRL) supplemented with lactalbumin hydrolysate, yeastolate, 0.1% Pluronic F68, 10% heat-inactivated fetal bovine serum (HyClone Laboratories, Inc.) and 50 μg of gentamicin per ml. Cells were grown at 27°C in the bioreactor to a density of 1.5 × 10⁶ to 1.8 × 10⁶ cells/ml; cultures were then inoculated with virus at a multiplicity of infection of 1.0 and allowed to grow for an additional 32 to 36 h at 27°C. Cells typically reached a density of 2 × 10⁶ to 2.2 × 10⁶ cells/ml at the time of harvest. Hormone was added to the bioreactors for the last 6 to 8 h of infection as follows: R5020 (500 nM) for PR, dihydrotestosterone (500 nM) for AR, and triamcinolone acetonide (500 nM) for GR. Cells were harvested by centrifugation at 1,500 rpm for 15 min in 50-ml aliquots, washed once in TG buffer (10 mM Tris-HCl [pH 8.0], 10% glycerol), and frozen as pellets at −80°C.

Expression of recombinant VDR, RXRα, and RARγ in yeast.

Human VDR, RXRα, and RARγ were expressed as polyhistidine-tagged proteins in the protease-deficient yeast strain BJ2168 from a copper-inducible promoter on 2μm plasmids as described previously (3). Briefly, transformed yeast were grown in 2-liter volumes to an A₆₀₀ of 1.0 in minimal selection medium, and expression was stimulated by addition of 100 μM copper sulfate for 16 h at 30°C. VDR cultures were also induced by addition of 1 μM 1,25-(OH)₂ D₃. Cells were then harvested and lysed with acid-washed glass beads in the following buffer: 10 mM sodium phosphate, pH 8.0; 0.4 M KCl; 0.5 mM phenylmethylsulfonyl fluoride (PMSF); aprotinin, 1 μg/ml; and 5 mM β-mercaptoethanol. Lysates were centrifuged at 100,000 × g for 45 min at 4°C to yield a soluble supernatant.

Purification of polyhistidine-tagged nuclear receptors with metal ion affinity columns.

For polyhistidine-tagged steroid receptors expressed from baculovirus vectors, Sf9 insect cells were lysed in the following buffer: 20 mM Tris-HCl, pH 8.0; 350 mM NaCl; 10 mM imidazole; 5% glycerol; and a cocktail of protease inhibitors (16). All procedures were done at 0 to 4°C. Cell lysates were centrifuged at 100,000 × g for 30 min, and the supernatant was taken as a soluble whole-cell extract. Whole-cell extract was passed once over Talon resins in a column at a flow rate of 1 to 2 ml/min. The resins were washed with cell lysis buffer until the optical density at 280 nm returned to the buffer baseline. Bound receptors were eluted under nondenaturing conditions by competition with 100 mM imidazole. Receptors were eluted into siliconized tubes to prevent binding to surfaces, and dithiothreitol (DTT; 1 mM), zinc chloride (1 μM), and MgCl₂ (1 mM) were added immediately to stabilize receptor DNA and steroid binding activity. Samples were stored at −80°C in aliquots and remained stable for DNA binding activity for several months if not repeatedly frozen and thawed.

Baculovirus-expressed human estrogen receptor was a gift from Angelo Notides (Rochester University) and was prepared as previously described (39). Briefly, Sf9 insect cells containing expressed ER bound to 17β-estradiol were lysed by freeze-thawing in TDEE buffer (40 mM Tris-Cl, pH 7.4; 1 mM EDTA; 1 mM DTT; 1 mM EGTA), and the cell lysates were centrifuged at 28,000 × g followed by centrifugation at 143,000 × g to obtain soluble cytosol. ER was precipitated with 40% saturated ammonium sulfate, and the pellet was redissolved in TDEE buffer and circulated over a DNA affinity column constructed with an estrogen response element (ERE) oligonucleotide. The column was washed several times in TDEE buffer containing 200 mM KCl, and bound ER was eluted with a linear 200 to 800 mM KCl gradient.

Purification of recombinant polyhistidine-tagged human VDR and human RXRα from soluble yeast cell lysates was done with nickel chelation affinity columns (Qiagen) according to the manufacturer’s recommended protocol. Yeast whole-cell extracts were bound to resins in a column at 4°C, and the column was equilibrated and washed in the yeast cell lysis buffer described above with the addition of 10% glycerol. Bound receptors were eluted in the cell lysis buffer with a pH 4.0 to 6.0 linear gradient. Collected fractions were immediately neutralized with 1 M Tris base (pH 8.5). Polyhistidine-tagged human RARγ was purified by using Talon resins as described above for steroid receptors except that the lysates were loaded onto the Talon resins and the resins were washed in the lysis buffer containing 2 mM imidazole. The eluted receptor was then diluted to reduce imidazole and was repurified on fresh Talon resins. VDR was bound to 1,25-(OH)₂ D₃ in yeast cells prior to extraction and purification. RXRα and RARγ were purified in their unliganded state.

Purification of HMG-1 and HMG-2.

A non-acid extraction method was used to purify HMG-1 and HMG-2 from calf thymus by a modification of the method originally defined by Adachi et al. (1). Fresh calf thymus (60 g) was cut into small pieces, trimmed of connective tissue, and homogenized in a stainless-steel blender in 6 volumes (tissues to buffer) of 10 mM sodium citrate buffer containing 0.15 M NaCl and 1 mM PMSF. The homogenate was filtered through cheesecloth and centrifuged for 15 min at 2,000 × g. The pellet was then washed three times by resuspension in homogenization buffer and centrifugation at 2,000 × g. The pellet was resuspended in 180 ml of 50 mM Tris-HCl (pH 7.8) containing 3 mM PMSF and then centrifuged for 10 min at 6,000 × g. This step was repeated. The resulting pellet containing nuclei and other membrane fractions was resuspended in a buffer (10 mM Tris-HCl, pH 7.8; 0.35 M NaCl; 5 mM DTT; 1 mM PMSF) in a glass-glass Dounce homogenizer. After homogenization, samples were left on ice for 1 h with brief intermittent vortexing to release 0.35 M NaCl-extractable nuclear proteins. The samples were then centrifuged at 5,000 × g for 20 min. The salt extraction of the nuclear pellet was repeated, and the two salt nuclear extracts were combined and centrifuged at 50,000 × g for 30 min. The supernatant was dialyzed overnight at 4°C against 10 mM Tris-HCl (pH 7.8) containing 1 mM DTT. The dialyzed salt nuclear extract was then passed over a PBE94 chromatofocusing column with a peristaltic pump at a flow rate of 1 to 2 ml/min. The column was washed and eluted with a linear 0 to 1.5 M NaCl gradient (in 10 mM Tris-HCl, pH 7.8; 5 mM DTT), and 1.0-ml fractions were collected and analyzed by sodium dodecyl sulfate (SDS)–12% polyacrylamide gel electrophoresis (PAGE) and Coomassie blue staining for the presence of HMG-1/-2. The peak fractions containing HMG-1/-2 were pooled, dialyzed against 10 mM Tris-HCl (pH 7.8)–1 mM DTT, and rechromatographed on a second PBE94 column. The fractions containing HMG-1 and HMG-2 were pooled and stored at −80°C.

Coimmunoprecipitation assay.

Purified calf thymus HMG-2 (1,000 ng) was incubated with purified PR-B (200 ng) for 1 h at 4°C. The samples were then incubated for 3 h at 4°C on an end-over-end rotator with a 100-μl suspension of protein A-Sepharose that was prebound with a rabbit polyclonal antibody (≈2 μg) to HMG-2. As controls to determine the extent of nonspecific binding, blank protein A-Sepharose-coated beads and anti-HMG-2 antibody-coated beads were incubated with PR-B (200 ng) in the absence of HMG-2. Beads were washed four times with TEDG_N100 (10 mM Tris base, pH 7.4; 1 mM EDTA; 1 mM DTT; 100 mM NaCl; 10% glycerol), transferred to a new tube, and washed twice more. Bound PR-B was eluted with SDS-sample buffer and analyzed by Western blotting with the PR-specific MAb 1294. The same coimmunoprecipitation assay was performed except that 200 ng of purified human VDR was used in place of PR-B and protein A-Sepharose eluates were analyzed for VDR by Western blotting with a rat MAb (9A7) to human VDR.

Immobilized metal affinity chromatography pull-down assays.

Baculovirus-expressed polyhistidine-tagged HMG-1 (10 μg) was incubated with non-histidine-tagged PR-B (8 μg) that was expressed in baculovirus and purified by MAb affinity chromatography as described in earlier studies (42, 46). Samples were incubated for 30 min at 4°C and then added to Talon resins in suspension and incubated for another 1 h at 4°C. Talon resins were washed four times in 20 mM Tris-HCl (pH 8.0)–10% glycerol–100 mM NaCl–15 mM imidazole, transferred to a new tube, and washed twice more. Bound proteins were eluted with SDS-sample buffer, and PR-B was detected by Western blotting with the PR-specific MAb 1294.

EMSA.

DNA binding was determined by electrophoretic mobility shift assay (EMSA) for PR and AR under the same conditions as described previously (42, 46, 47). Briefly, receptors in whole-cell or purified extracts of Sf9 cells (amounts per assay are indicated in the figure legends) were incubated for 1 h at 0 to 4°C with 0.3 ng of a ³²P-end-labeled glucocorticoid response element-progesterone response element (GRE-PRE) oligonucleotide (specific activity, 100,000 to 300,000 cpm/ng) in a DNA binding buffer containing 10 mM Tris-base (pH 7.5), 50 mM NaCl, 5 mM DTT, 2 mM MgCl₂, 1 mM EDTA, and 5% glycerol. The oligonucleotide was end labeled by filling in of 5′ single-stranded ends with the Klenow fragment of DNA polymerase by using [α-³²P]dATP and [α-³²P]dCTP. The binding reactions also contained 1 μg of gelatin (or albumin) as a carrier protein and 80 to 100 ng of competitor poly(dA-dT)-poly(dA-dT). All components of the binding reaction were preincubated for 30 min at 4°C prior to addition of the [³²P]DNA probe. After 1 h, DNA binding reactions (25 μl) were electrophoresed on 5% polyacrylamide (40:1 acrylamide/bisacrylamide ratio) gels in 0.5× TAE buffer (0.02 M Tris-acetate, pH 8.0; 0.5 mM EDTA) with cooling to maintain the gel temperature at 4°C. Gels were dried under vacuum and autoradiographed, and free [³²P]DNA and [³²P]DNA complexes were quantitated by direct scanning of the gels for radioactivity by a series 400 Molecular Dynamics PhosphorImager. DNA binding as determined by EMSA for other nuclear receptors was accomplished by similar methods with the following minor modifications. A different binding buffer that included 10 mM HEPES (pH 7.8), 50 mM KCl, 4 mM MgCl₂, and 12% glycerol was used with GR, ER, VDR, RARγ, and RXRα. Additionally, 2.5% glycerol was added to the polyacrylamide gel, and the electrophoresis buffer was 0.25× TBE (0.02 M Tris-borate, pH 8.0; 0.02 M boric acid; 0.5 M EDTA).

A palindromic GRE oligonucleotide was used as the DNA probe for PR, AR, and GR: 5′-gatcTTTGAGAACAAACTGTTCTTAAAACGAG-3′ and 3′-AAACTCTTGTTTGACAAGAATTTTGCTCctag-5′. The DNA probe for ER binding was a palindromic ERE oligonucleotide: 5′-gatcTCTTTGATCAGGTCACTGTGACCTGACTTTG-3′ and 3′-AGAAACTAGTCCAGTGACACTGGACTGAAACctag-5′. For RXR-RXR homodimer binding the probe was a DR-1 oligonucleotide: 5′-tcgaCAGGTCAGAGGTCAGTCGA-3′ and 3′-GTCCAGTCTCCAGTCAGCTgatc-5′. For VDR-RXRα heterodimer binding the probe was a DR-3 oligonucleotide: 5′-tcgaCAGGTCAAGGAGGTCAG-3′ and 3′-GTCCAGTTCCTCCAGTCgatc-5′. For RXRα-RARγ heterodimer binding the probe was a DR-5 oligonucleotide: 5′-tcgaCAGGTCACCAGGAGGTCAG-3′ and 3′-GTCCAGTGGTCCTCCAGTCagct-5′. In some experiments PR binding was done with a longer, 43-bp oligonucleotide containing a single GRE-PRE and flanking sequences from the mouse mammary tumor virus: 5′-gatcGGGTTTAAATAAGTTTATGGTTACAAACTGTTCTTAAAACAAG3′ and 3′-CCCAAATTTATTCAAATACCAATGTTTGACAAGAATTTTGTTCctag-5′.

SDS-PAGE and Western blots.

Receptors or HMG-1/-2 were subjected to SDS-PAGE on 12, 10, or 7.5% polyacrylamide gels as previously described (42, 46, 47), and protein bands were detected by staining with Coomassie blue or silver (21). Separated proteins were transferred to nitrocellulose membranes and incubated with the appropriate receptor-specific antibody overnight at 4°C. For mouse MAbs, Western blot detection was done with a secondary goat anti-mouse–horseradish peroxidase colorimetric method or by using rabbit anti-mouse IgG and ³⁵S-labeled protein A followed by autoradiography. With rabbit polyclonal antibodies, goat anti-rabbit–horseradish peroxidase (Cappel) was used for detection. For the rat 9A7 MAb to human VDR, the detection method was with rabbit anti-rat IgG followed by the addition of ³⁵S-labeled protein A and autoradiography.

Expression plasmids and reporter gene constructs.

The expression plasmids pHMG-1 and pHMG-2 contain genomic clones of mouse HMG-1 and HMG-2, respectively, under the control of their own constitutive promoters that were then cloned into the EcoRI site of pBluescript KS(+) as previously described (73). pBluescript KS(+) from Stratagene was used as an empty vector control. A mammalian-cell expression plasmid for human PR-B (pPR-B) was provided by Donald McDonnell (Duke University, Durham, N.C.) (64), an expression plasmid for human AR (p5HBL-AR-A) was provided by Elizabeth Wilson (University of North Carolina, Chapel Hill, N.C.), and an expression plasmid for GR (pSVGR) was provided by Ron Evans (Salk Institute). Human VDR expression plasmid (pRShVDR) for mammalian cells was under the control of the Rous sarcoma virus promoter (76).

Two progesterone-responsive reporter plasmids were used in the transfection assays. DHRE-E1b-CAT contains two optimal GRE-PRE elements linked to the TATA box of the adenovirus E1b gene and the chloramphenicol acetyltransferase (CAT) gene. PRE₂tk-LUC contains two PREs linked to the herpes simplex virus thymidine kinase promoter and the luciferase (LUC) reporter gene as previously described (64). Three VDRE (vitamin D-responsive element) LUC reporters were used. These included the human 25-hydroxyvitamin D₃–24-hydroxylase [24 (OH)ase] promoter (−1177 to −22), which contains two VDREs linked to LUC as previously described (76), a synthetic VDRE subcloned into a mouse mammary tumor virus (MMTV)-LUC vector (VDRE-1ΔMTV-LUC) that has the GREs deleted (76), and a synthetic DR-3 oligonucleotide cloned into a minimal tk-LUC reporter vector (DR3-tk-LUC).

Cell culture and transient transfections.

COS1 cells were maintained in Dulbecco modified Eagle medium (DMEM; Gibco-BRL) supplemented with 10% fetal bovine serum (HyClone) and were plated in multiwell dishes (six-well dishes; Falcon Plastics) at a density of 175,000 to 200,000 cells/well. HeLa cells were plated at the same density in DMEM supplemented with 5% fetal bovine serum. Cells were transiently transfected by an adenovirus-mediated method described previously (4). Purified defective adenovirus particles, covalently coupled to poly-l-lysine (provided by Nancy Weigel, Baylor College of Medicine, Houston, Tex.), were mixed with plasmid DNA for 30 min at room temperature in 20 mM HEPES buffer (pH 7.8) followed by the addition of poly-l-lysine at 2.4 μg/ml for an additional 30 min at room temperature. Just prior to transfection, culture medium was removed from the wells and replaced with 3 ml of serum-free DMEM per well, and the adenovirus-plasmid mixture was added directly to the medium at a multiplicity of infection of 250 to 400 viral particles/cell. Cells were then incubated for 2 h at 37°C, and then 3 ml of DMEM supplemented with 10% fetal bovine serum was added per well to bring the final serum concentration to 5%. The cells were then incubated for another 24 h at 37°C. After 24 h the cells were treated for another 24 h with and without hormone. At 48 h after transfection, cell monolayers were rinsed with CAT-LUC wash buffer (40 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM EDTA), and the cells were lysed in the well by the addition of 300 μl of cell lysis buffer (20 mM potassium phosphate, pH 7.4; 5 mM MgCl₂; 0.5% Triton X-100) per well; the lysates were then measured for CAT or LUC activity. To estimate the percentage of transfected cells, parallel cells in six-well dishes were transfected with a cytomegalovirus (CMV)–β-galactosidase (β-Gal) reporter gene. At 48 h after transfection, cells were fixed in the well with 0.5% glutaraldehyde in phosphate-buffered saline and incubated with a 2% X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) staining solution (4). The percentage of blue-stained cells averaged 15% for HeLa cells and 30% for COS1 cells. To control for variation in transfection efficiency, the cells were routinely cotransfected with a CMV–β-Gal internal control plasmid (2 ng/well). Cotransfection cultures with receptors and HMG-1/-2 expression plasmids also contained appropriate amounts of empty control vectors so that all cultures received the same amount of total plasmid DNA.

HMG-1 and the closely related HMG-2 facilitate the sequence-specific DNA-binding activity of recombinant purified human PR.

Our previous studies showing that HMG-1 increased the DNA binding activity of PR were done with a nonfusion baculovirus-expressed PR purified by MAb affinity chromatography (42, 46). Therefore, before analyzing the effects of HMG-1 on other nuclear receptors, we determined whether polyhistidine-tagged PR purified by metal affinity resins would respond in a similar manner to HMG-1 as had antibody-purified PR. Crude and highly purified preparations of baculovirus expressing polyhistidine-tagged PR-A (Fig. ​(Fig.1A)1A) were analyzed by EMSA for binding to a PRE oligonucleotide. As with our results with nonfusion MAb-purified PR (42, 46), polyhistidine-tagged PR-A in crude extracts of Sf9 insect cells bound to the PRE probe, whereas an equal amount of purified polyhistidine-tagged receptor exhibited no DNA binding activity (Fig. ​(Fig.4A).4A). The addition of calf thymus nuclear extracts or purified calf thymus HMG-1 stimulated the formation of PR-DNA complexes (Fig. ​(Fig.4A).4A). As controls for the general effect of other proteins on the stability of purified PR, the addition of unrelated proteins such as albumin or insulin had no effect on PR-DNA binding (Fig. ​(Fig.4A).4A). The DNA complex stimulated by HMG-1 is specific, as demonstrated by supershifts with a MAb to PR and by competition with unlabeled homologous PRE oligonucleotide but not by an ERE oligonucleotide (Fig. ​(Fig.4A).4A). Purified recombinant baculovirus-expressed HMG-1 exhibited stimulatory activity similar to that of native HMG-1 purified from calf thymus, indicating that minor contaminants in calf thymus preparations are not responsible for stimulating PR-DNA binding activity (data not shown). In previous studies we observed that bacterially expressed and purified HMG-1 stimulated PR-DNA binding (42). The fact that one source of purified cellular HMG-1 and two sources of purified recombinant HMG-1 all have similar activities strongly indicates that the stimulation of PR-DNA binding is an intrinsic property of HMG-1/-2 and is not due to contaminating proteins.

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FIG. 4

HMG-1/-2 stimulate the sequence-specific DNA binding activity of all steroid receptors tested. (A) Equal amounts (30 nM) of polyhistidine-tagged PR-A in Sf9 whole-cell extract (WCE) or affinity-purified PR-A were analyzed for binding to a ³²P-labeled palindromic GRE-PRE oligonucleotide by EMSA. Lanes contain Sf9 whole-cell extract (WCE), purified PR alone (−), or purified PR plus 1 μg of ovalbumin (+Oval), 1 μg of nuclear extract (+NE) from calf thymus, 1 μg of insulin (+INS), or 300 ng of purified calf thymus HMG-1 (+HMG-1). The HMG-1-stimulated PR-DNA complexes were supershifted by the PR-specific MAb, AB-52, and were competed by a 100-fold molar excess of the PRE oligonucleotide (100×sp) but not by an ERE oligonucleotide (100×ns). (B) Equal amounts (70 nM) of baculovirus-expressed AR in WCE and affinity-purified AR were analyzed for binding to the GRE-PRE oligonucleotide by EMSA as described for panel A. (C) Equal amounts (10 nM) of baculovirus-expressed ER in WCE and affinity-purified ER were analyzed for binding to an ERE oligonucleotide by EMSA as described for panel A with or without the addition of 300 ng of purified calf thymus HMG-1. (D) Equal amounts (200 nM) of baculovirus-expressed GR in WCE and affinity-purified GR were analyzed for binding to the GRE-PRE by EMSA as described for panel A. All receptors were bound to their cognate (see Materials and Methods) hormonal ligands in Sf9 cells during expression.

In our previous studies, only HMG-1 was analyzed (42). To compare the activity of HMG-1 and the closely related HMG-2, PR-DNA binding assays were performed with a constant amount of purified PR-A and various amounts of purified HMG-1 or HMG-2 (see Fig. ​Fig.3A).3A). Both HMG-1 (Fig. ​(Fig.3B)3B) and HMG-2 (Fig. ​(Fig.3C)3C) stimulated the formation of specific PR-DNA complexes in a dose-dependent manner. Maximal stimulation was observed with the addition of between 200 and 400 ng for both HMG-1 and HMG-2, which is approximately a 5- to 10-fold molar excess over PR. HMG-1 and HMG-2 exhibited slight differences in activity in two respects, however. First, HMG-2 was consistently observed to stimulate a higher maximal level of PR-DNA complexes (Fig. ​(Fig.3B3B and C). Second, HMG-2 appeared to stimulate cooperative binding of PR to DNA, a finding that was not evident with HMG-1. The dose-dependent increase in PR-DNA binding caused by the addition of HMG-1 occurred gradually over the entire range of HMG-1 concentrations. In contrast, nearly all of the stimulation of PR-DNA complexes produced by addition of HMG-2 was obtained over a much narrower concentration range, i.e., between 100 and 200 ng of HMG-2 per assay (Fig. ​(Fig.33C).

HMG-1/-2 facilitate the sequence-specific DNA binding activity of all steroid receptors tested.

We next analyzed the influence of HMG-1/-2 on the DNA binding activities of other steroid receptors. Because PR, GR, and AR all recognize a common GRE, these receptors were assayed for binding to the same palindromic GRE oligonucleotide probe. ER binding was assessed with a palindromic ERE probe. As with the results with PR, recombinant baculovirus-expressed AR, GR, and ER in crude Sf9 cell extracts bound specifically to their target DNA probes. Equal amounts of purified preparations of these same receptors exhibited a loss of DNA binding activity that was restored by the addition of either HMG-1 or HMG-2 but not by the addition of other unrelated proteins (Fig. ​(Fig.4B4B to D). The DNA complexes stimulated by HMG-1 or HMG-2 were specific, as demonstrated by supershifts with the appropriate receptor-specific antibody and by competition with homologous unlabeled DNA probes (Fig. ​(Fig.4B4B to D). Similar to results with PR, HMG-1 and HMG-2 were found to be interchangeable with respect to stimulating the DNA binding activity of other purified steroid receptors (data not shown).

In previous studies with PR purified by MAb affinity chromatography, we observed that receptor alone was able to bind to ³²P-labeled GRE-PRE but with low affinity requiring a high receptor concentration for detection by EMSA. The addition of purified HMG-1 increased the apparent affinity of PR for its target DNA sequences and thus was most effective in stimulating DNA binding activity at low receptor concentrations (42, 46). To determine whether HMG-1/-2 also increased the binding affinity of other steroid receptors for target DNA, saturation DNA binding assays were performed with purified GR, AR, and ER in the presence or absence of HMG-1/-2. Purified polyhistidine-tagged PR-A was used as a reference. These experiments were done by varying the concentration of purified receptors against a constant amount of [³²P]DNA probe under equilibrium binding conditions. Specific receptor-DNA complexes at each receptor concentration were quantitated by phosphorimage scanning of the gels, and the data were plotted as the percent upshifted DNA relative to total DNA. Figure ​Figure55 (A to D) shows that each purified steroid receptor bound to specific DNA with low affinity, generating complexes only at high receptor concentrations. Addition of either HMG-1 or HMG-2 but not an unrelated protein such as ovalbumin caused a substantial leftward shift of the binding curves for all purified steroid receptors. The relative DNA binding affinity and the fold increase caused by the addition of HMG-1/-2 varied for each receptor. This could be due to differences in intrinsic binding affinities of each receptor for target DNA or to differences in the estimated concentration of each purified receptor. Interestingly, AR showed no detectable binding in the absence of HMG-1/-2 at any of the concentrations tested, whereas other steroid receptors produced low levels of DNA binding in the absence of HMG-1/-2. Thus, the fold increase in the apparent DNA binding affinity caused by the addition of HMG-1 or HMG-2 was greatest with AR. We also observed that the concentrations of AR and GR in the presence of HMG-1/-2 required to saturate the DNA binding sites were higher than that required for PR and ER and that ER achieved saturation at the lowest concentration. This suggests that AR and GR have lower binding affinities than PR for the same target DNA and that ER has the highest affinity for target DNA. Alternatively, variation in the percentage of purified receptors that are active could also contribute to these concentration differences. Nevertheless, all four purified steroid receptor preparations exhibited a substantial increase in affinity for target DNA in response to the addition of HMG-1 or HMG-2.

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FIG. 5

Saturation DNA binding analysis of purified nuclear receptors in the presence or absence of HMG-1/-2. The concentrations of purified receptors indicated in the figure were varied in DNA binding reactions against a constant amount (0.3 ng) of ³²P-labeled oligonucleotide probe, and DNA binding was analyzed by EMSA. Each receptor was assayed alone (−HMG-1 or −HMG-2) or with the addition of 150 to 300 ng of HMG-1, HMG-2, or ovalbumin (+OVAL). The oligonucleotide probe for PR-A (panel A), GR (panel B), and AR (panel C) was a palindromic GRE-PRE. A palindromic ERE oligonucleotide was used as the probe for ER (panel D), a DR-3 oligonucleotide was used for the VDR-RXR heterodimers (panel E), and a DR-1 probe was used for the RXR homodimers (panel F). Purified receptors were quantitated by protein Bradford assay and by comparing silver-stained receptor band intensities with those of known amounts of purified molecular weight protein standards. The specific DNA complexes at each concentration of receptor were quantitated by phosphorimage analysis and plotted as the percentage of total DNA.

HMG-1/-2 have no effect on the binding of nonsteroid nuclear receptors to direct-repeat target DNA sequences.

We next examined the influence of HMG-1/-2 on binding of human VDR and RARγ to DR target DNA sequences. EMSA showed that purified VDR did not bind to a DR-3 probe (Fig. ​(Fig.6A)6A) and that purified RARγ failed to interact with a DR-5 probe (Fig. ​(Fig.6B).6B). In contrast to the results with steroid receptors, the addition of HMG-1 or HMG-2 had no effect on the DNA binding activities of VDR and RARγ (Fig. ​(Fig.6).6). As expected, the addition of purified RXR stimulated the DNA binding activity of purified VDR and RAR by a heterodimerization mechanism, as shown by supershifts with RXR-, VDR-, and RAR-specific antibodies (Fig. ​(Fig.6).6). Competition with excess unlabeled homologous and unrelated oligonucleotides demonstrates that the RXR-induced complexes are specific (Fig. ​(Fig.6A6A and B). The addition of HMG-1 or HMG-2 also had no effect on the DNA binding activity of RXR-VDR and RXR-RAR heterodimers (Fig. ​(Fig.6A6A and B). To determine whether HMG-1/-2 might in fact stimulate this class of nonsteroid nuclear receptors but simply require a higher concentration than is needed to stimulate steroid receptors, increasing amounts of HMG-2 were added to VDR-RXR heterodimer DNA binding reactions. As shown in Fig. ​Fig.6C,6C, at concentrations three times higher (900 ng) than are required to maximally stimulate PR-DNA binding, HMG-2 had no effect on the sequence-specific DNA binding activity of VDR-RXR heterodimers (Fig. ​(Fig.6C).6C). Also, the HMG-2 antibody did not supershift VDR-RXR DNA complexes in the presence of large amounts of HMG-2, suggesting that HMG-2 is not recruited to the complex (Fig. ​(Fig.6C).6C).

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FIG. 6

HMG-1/-2 has no influence on sequence-specific DNA binding activity of purified human VDR and RARγ. (A) Human VDR expressed and purified from yeast cells was incubated for 1 h at 4°C with 0.3 ng of a [³²P]DR-3 probe, and samples were analyzed by EMSA. A single concentration of purified VDR (60 nM) was incubated alone (−) or with the addition of 150 ng of purified HMG-1, 1 μg of ovalbumin (Oval), 50 ng of purified RXRα, or 50 ng of RXRα plus 150 ng of purified HMG-1. A supershift of RXR-induced DNA complexes with an antibody to RXRα (RXRab) shows that RXR is a component of the stimulated DNA complex. The specificity of the DNA complexes is shown by the lack of competition with a 100-fold molar excess of an unlabeled nonspecific DNA (100×ns) compared to an unlabeled DR-3 (100×DR-3). (B) Purified human RARγ (60 nM) expressed and purified from yeast cells was incubated with 0.3 ng of a [³²P]DR-5 probe, and samples were analyzed by EMSA as for panel A. (C) Purified VDR and RXR were incubated with the DR-3 probe as for panel A. Supershifts of the RXR-VDR heterodimer-DNA complex are shown with antibodies to VDR (VDR ab) and RXR (RXR ab). Increasing amounts of purified HMG-2 (300, 600, and 900 ng) were added to the VDR-RXR heterodimer-DNA binding reaction. At the intermediate amount of HMG-2 (i.e., 600 ng), DNA binding reactions were incubated with two concentrations of HMG-2 antibody (HMG-2 ab) or a control unrelated antibody (Control ab).

Saturation DNA binding analyses were performed with purified RXRα-VDR and RXRα-RARγ heterodimers in the presence or absence of HMG-1. The apparent binding affinity of RXRα-VDR heterodimers for a DR-3 element (Fig. ​(Fig.5E)5E) and the affinity of RXRα-RARγ heterodimers for a DR-5 probe (not shown) were not affected by the addition of HMG-1. Thus, HMG-1 and HMG-2 are not able to functionally substitute for RXR as coregulators of VDR and RAR DNA binding, and these two proteins did not influence the DNA binding activity of RXR-VDR or RXR-RAR heterodimers.

RXR is capable of binding as a homodimer to DR-1 elements. However, this binding is weaker than that of RXR heterodimers for their cognate DR sites. Therefore, we also asked whether HMG-1/-2 were capable of stimulating RXR homodimer binding to DNA. As shown by the saturation DNA binding analysis in Fig. ​Fig.5F,5F, HMG-1 had no influence on the low binding activity of purified RXR for a DR-1 probe. Thus, based on an analysis of four different steroid receptors and three nonsteroid nuclear receptors, it appears that HMG-1/-2 are capable of stimulating only the sequence-specific DNA binding activity of steroid receptors.

HMG-1 and HMG-2 transiently interact with PR and are recruited by PR to the DNA complex.

To further investigate the mechanism by which HMG-1/-2 stimulate the apparent binding affinity of steroid receptors for HREs, we focused our analysis on PR. Using short PRE oligonucleotide probes, we previously observed that stimulation of PR-DNA binding by HMG-1 was not accompanied by a decrease in electrophoretic mobility of the DNA complex, nor were stimulated complexes supershifted by the addition of antibodies to HMG-1 (42, 46). This suggested that HMG-1 is a less-stable component of the DNA complex than PR and that it dissociates during electrophoresis. However, in the context of a longer DNA fragment containing a single PRE, the addition of HMG-1 did result in reduced mobility of some of the stimulated PR-DNA complexes, which was further supershifted by anti-HMG-1 MAb (47). To test whether HMG-2 can also associate with the PR-DNA complex, we used a longer PRE oligonucleotide probe than that used in Fig. ​Fig.33 to ​to55 (43 bp versus 32 bp). As shown in Fig. ​Fig.7A,7A, purified HMG-2 alone did not bind to the 43-bp PRE probe at any of the concentrations tested, nor were complexes formed with purified PR alone at a submaximal receptor concentration. The addition of purified PR and HMG-2 together resulted in a synergistic stimulation of DNA complexes that contained both HMG-2 and PR, as evidenced by supershifts with PR (AB-52)- or HMG-2-specific antibodies (Fig. ​(Fig.7A).7A). As a control, an antibody (h151) to ER had no effect on the electrophoretic mobility of the complex. These results show that HMG-2 can associate with the PR-DNA complex. Furthermore, the inability of HMG-2 to associate with the PRE until receptor was added indicates that HMG-2 is recruited to the complex by PR.

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FIG. 7

Association of HMG-2 with the PR-DNA complex and direct binding of HMG-1/-2 to PR in the absence of DNA. (A) HMG-2 is recruited to the PR-DNA complex. A 43-bp oligonucleotide containing the distal-most GRE-PRE and flanking sequences of MMTV was used as the DNA probe for EMSA. The DNA probe (0.3 ng) was incubated with increasing amounts of purified HMG-2 alone (100 to 300 ng), with purified PR-A alone (30 nM), or with purified PR-A (30 nM) plus 300 ng of HMG-2, either without (none) or with antibody to PR (AB-52), ER (h151), or HMG-2 (HMG-2ab; rabbit antisera added in increasing amounts). (B) (Left panel) Baculovirus-expressed polyhistidine-tagged HMG-1 was incubated with nonfusion recombinant PR-B, and the samples were bound to Talon resins. Resins were washed six times in a buffer containing 100 mM NaCl and 15 mM imidazole, and bound proteins were eluted with SDS-sample buffer and analyzed by Western blotting with the 1294 MAb to PR. As a control for nonspecific binding, PR-B was incubated with Talon resins in the absence of polyhistidine-tagged HMG-1. As a positive control for a known interacting protein, PR-B was incubated with polyhistidine-tagged SRC-1. Purified PR-B (middle panel) or VDR (right panel) was incubated with calf thymus HMG-2, and samples were immunoprecipitated with a rabbit antibody to HMG-2 by using protein A-Sepharose as an absorbent. The protein A-Sepharose beads were washed six times in a buffer containing 100 mM NaCl, and bound proteins were eluted with SDS-sample buffer and analyzed by Western blotting with the 1294 MAb to PR (middle panel) or the 9A7 MAb to human VDR (right panel). The leftmost lane in each of these two panels is the assay input of PR-B or VDR.

We next asked whether HMG-1/-2 can interact directly with PR in the absence of DNA. This was tested by a pulling down of the PR with histidine-tagged HMG-1 and by coimmunoprecipitation of PR and HMG-2 with an anti-HMG-2 antibody. As shown in Fig. ​Fig.7B,7B, a weak but specific association of PR-B with immobilized polyhistidine–HMG-1 was detected. As a positive control for a known receptor-interacting protein, Fig. ​Fig.7B7B also shows PR-B association with immobilized polyhistidine-tagged steroid receptor coactivator (SRC-1–his). With a rabbit polyclonal antibody specific to HMG-2, PR-B was coimmunoprecipitated with HMG-2 in a specific manner when the two proteins were added together. PR was not immunoprecipitated by the HMG-2 antibody in the absence of HMG-2 (Fig. ​(Fig.7B).7B). Interestingly, HMG-2 did not interact with VDR as indicated by coimmunoprecipitation assay (Fig. ​(Fig.7B),7B), nor did it affect VDR-DNA binding activity, suggesting that the transient interaction of HMG-1/-2 with PR may be meaningful.

HMG-1/-2 stimulate PR-mediated gene activation in mammalian cells.

To determine whether HMG-1/-2 can affect the transcriptional activity of PR in vivo, COS1 cells were cotransfected with expression plasmids for hPR-B, HMG-1, or HMG-2, and a progesterone-responsive reporter construct containing two GREs-PREs fused to the TATA box of E1b and the CAT reporter gene (DHRE-E1b-CAT). PR-B in general is a stronger transcriptional activator than PR-A; however, this differential activity is both cell-type and promoter context specific (64, 69). With this simple GRE-PRE promoter, PR-B in COS1 cells is a much stronger activator than PR-A (not shown). Thus, our studies have focused on the effect of HMG-1/-2 on the activity of PR-B. When COS1 cells were transfected with phPR-B alone, addition of the synthetic progestin R5020 resulted in an ∼10-fold stimulation of CAT activity (Fig. ​(Fig.8A).8A). Upon cotransfection with phPR-B and increasing amounts of the HMG-1 expression plasmid (pHMG-1), R5020 stimulation of CAT activity was increased in a dose-dependent manner by another sevenfold at the highest amount of transfected pHMG-1 (7.03-fold ± 1.9 standard error of the mean [SEM], n = 11). Cotransfection with an empty vector control had minimal effect on R5020 stimulation of the DHRE-E1b-CAT reporter gene (Fig. ​(Fig.8A).8A). In the absence of hormone small increases in reporter gene activity were observed when cotransfecting cells with phPR-B and pHMG-1. The increase in R5020 induction by cotransfected HMG-1 is PR dependent and is not due to an effect on the basal promoter activity of the reporter gene. This is shown by the lack of stimulation of CAT activity when cells were transfected with pHMG-1 in the absence of hPR-B (Fig. ​(Fig.8A).8A). These results demonstrate that cotransfection of HMG-1 substantially increases hormone-dependent PR-mediated transactivation without affecting the basal promoter activity of the reporter gene.

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FIG. 8

Ectopically expressed HMG-1 or HMG-2 stimulates the transcriptional activity of human PR in mammalian cells. (A) COS1 cells were transfected with a PR-B expression plasmid (phPR-B; 0.5 ng/well), increasing amounts of an HMG-1 expression plasmid (pHMG-1; 1 to 20 ng/well) or an empty vector control (1 to 20 ng/well), and the DHRE-E1b-CAT reporter plasmid (500 ng/well). Cells were treated with or without R5020 (100 nM) for the last 24 h of transfection. In some wells, cells were cotransfected with increasing amounts of pHMG-1 (1 to 20 ng/well) and no PR-B (−PR-B). CAT activity was calculated as counts per minute of ³H-labeled acetyl coenzyme A converted per microgram of total protein, and the values shown are averages from three independent experiments. (B) COS1 cells were transfected with various amounts of phPR-B expression plasmid in the presence or absence of pHMG-1 (20-fold molar excess over phPR-B). At 24 h after transfection, cells were treated for the next 24 h with or without R5020 (100 nM), and CAT activity was measured and calculated as the ratio of CAT activity to β-Gal activity of the internal control plasmid. The results are average values from duplicate transfection wells from a single experiment and are representative of two independent experiments. (C) COS1 cells were transfected with phPR-B (1 ng/well), DHRE-E1b-CAT reporter (500 ng/well), various amounts of pHMG-1 and pHMG-2, or an empty control vector (0.5- to 20-fold molar excess over the PR expression plasmid). The CAT activity in each treatment group was calculated as the fold R5020 induction over that with no hormone. The data were expressed as relative CAT activity, setting the hormone-induced level obtained in cells transfected with PR alone as the 100% value. The values are averages from four independent experiments. (D) HeLa cells were transfected with phPR-B (10 ng/well), increasing amounts of either pHMG-1 or the empty control vector (1.0- to 20-fold molar excess over phPR-B), and the DHRE-E1b-CAT (500 ng/well). The relative CAT activity was calculated as for panel C. The values are averages from duplicate transfections from a single experiment that is representative of three independent experiments.

As shown in Fig. ​Fig.8A,8A, cells were transfected with a suboptimal level of phPR-B (0.5 ng/well). To determine whether the stimulatory effect of HMG-1 on PR transactivation occurs only with subthreshold levels of receptor or whether it can also affect the activity of higher levels of receptor, COS1 cells were cotransfected with a constant ratio of pHMG-1 (20-fold molar excess over PR plasmid) and increasing amounts of phPR-B. As shown in Fig. ​Fig.8B,8B, pHMG-1 increased R5020 induction of DHRE-E1b-CAT at all of the amounts of transfected phPR-B tested, indicating that HMG-1 stimulates the maximal transcriptional activity of PR.

Since HMG-1 and HMG-2 are interchangeable with respect to stimulating PR binding to PREs in vitro, we also tested whether coexpression of HMG-2 affects the transcriptional activity of PR in whole cells. As shown in Fig. ​Fig.8C,8C, R5020 induction of the DHRE-E1b-CAT reporter gene was stimulated in a similar dose-dependent manner by cotransfection of COS1 cells with phPR-B and increasing amounts of either pHMG-1 or pHMG-2. R5020-dependent, PR-mediated transcription was increased fivefold by the highest amount of pHMG-1 and sevenfold (7.27 ± 2.8 SEM, n = 4) by pHMG-2 (Fig. ​(Fig.8C).8C). Thus, consistent with the in vitro DNA binding results, both HMG-1 and HMG-2 are capable of increasing the transcriptional activity of PR in vivo, with HMG-2 tending to have a greater effect.

To determine whether the effect of HMG-1/-2 on PR activity in vivo extends to other cell types and target promoters, cotransfection experiments were conducted in HeLa cells and with another progestin-inducible reporter construct (PRE₂-tk-LUC). In HeLa cells, PR-mediated activation of DHRE-E1b-CAT was stimulated in a dose-dependent manner with increasing amounts of cotransfected pHMG-1 (Fig. ​(Fig.8D).8D). In multiple independent experiments, it was found that the largest amount of pHMG-1 stimulated PR activity in HeLa cells by ca. fourfold (3.5 ± 0.044 SEM, n = 3). In COS1 cells, cotransfected pHMG-1 stimulated R5020-dependent PR-mediated activation of PRE₂-tk-LUC by a factor of ca. 10-fold (10.7 ± 2.3 SEM, n = 9). These results provide strong evidence that HMG-1/-2 have a general role in PR function in vivo that does not appear to be cell type or target gene restricted.

The first three authors made equal contributions to this work.

This work was supported in part by the Tissue Culture/Monoclonal Antibody Core facility of the University of Colorado Cancer Center (Cancer Center Core grant P30 CA46934) and by Public Health Service grant NCI-CA 46938 (D.P.E.).

We thank Kurt Christensen, Lori Sherman, Suzanne Meintzer, and Nicole Flessner for expert technical assistance and Elizabeth Wilson, University of North Carolina at Chapel Hill, for providing baculovirus AR expression vectors and for helpful suggestions and critical reading of the manuscript.