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Br J Cancer. 1991 Oct; 64(4): 741–744.
doi: 10.1038/bjc.1991.390
PMCID: PMC1977711
PMID: 1654986

Epidermal growth factor receptor in lung malignancies. Comparison between cancer and normal tissue.

R. Dittadi, M. Gion, V. Pagan, A. Brazzale, O. Del Maschio, A. Bargossi, A. Busetto, and G. Bruscagnin
Author information Copyright and License information PMC Disclaimer

Abstract

Epidermal growth factor receptors (EGFr) were measured using a radioligand binding assay, in membrane preparations from 51 human non-small cell lung cancers and in normal tissue of the same patients. The binding characteristics of EGFr were similar in tumour and normal lung membranes (range of dissociation constant of high affinity sites: 0.1-0.6 nM). However, the concentrations in tumours (median, 16.4 fmol mg-1 of protein; range, 1.5-176) were significantly higher than in normal tissues (median, 7.4 fmol mg-1 of protein; range, 1.9-13.4). The receptor levels in normal tissue were normally distributed. It was therefore possible to define a normal/pathologic cut-off level (12.9 fmol mg-1 of protein). In 57% of cases EGFr in cancer was higher than the cut-off. No relationships were found between receptor concentrations and positivity rates of EGFr and histology, stage, lymph node positivity and pT. A trend for a direct relation between receptor positivity and grading was found.

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Selected References

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  • Berger MS, Gullick WJ, Greenfield C, Evans S, Addis BJ, Waterfield MD. Epidermal growth factor receptors in lung tumours. J Pathol. 1987 Aug;152(4):297–307. [PubMed] [Google Scholar]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed] [Google Scholar]
  • Carpenter G. The biochemistry and physiology of the receptor-kinase for epidermal growth factor. Mol Cell Endocrinol. 1983 Jul;31(1):1–19. [PubMed] [Google Scholar]
  • Carpenter G, Cohen S. Epidermal growth factor. Annu Rev Biochem. 1979;48:193–216. [PubMed] [Google Scholar]
  • Cerny T, Barnes DM, Hasleton P, Barber PV, Healy K, Gullick W, Thatcher N. Expression of epidermal growth factor receptor (EGF-R) in human lung tumours. Br J Cancer. 1986 Aug;54(2):265–269. [PMC free article] [PubMed] [Google Scholar]
  • Cooper JA, Bowen-Pope DF, Raines E, Ross R, Hunter T. Similar effects of platelet-derived growth factor and epidermal growth factor on the phosphorylation of tyrosine in cellular proteins. Cell. 1982 Nov;31(1):263–273. [PubMed] [Google Scholar]
  • Dazzi H, Hasleton PS, Thatcher N, Barnes DM, Wilkes S, Swindell R, Lawson RA. Expression of epidermal growth factor receptor (EGF-R) in non-small cell lung cancer. Use of archival tissue and correlation of EGF-R with histology, tumour size, node status and survival. Br J Cancer. 1989 May;59(5):746–749. [PMC free article] [PubMed] [Google Scholar]
  • Dittadi R, Gion M, Brazzale A, Bruscagnin G. Radioligand binding assay of epidermal growth factor receptor: causes of variability and standardization of the assay. Clin Chem. 1990 Jun;36(6):849–854. [PubMed] [Google Scholar]
  • Downward J, Parker P, Waterfield MD. Autophosphorylation sites on the epidermal growth factor receptor. Nature. 1984 Oct 4;311(5985):483–485. [PubMed] [Google Scholar]
  • Feldman HA. Mathematical theory of complex ligand-binding systems of equilibrium: some methods for parameter fitting. Anal Biochem. 1972 Aug;48(2):317–338. [PubMed] [Google Scholar]
  • Gamou S, Hunts J, Harigai H, Hirohashi S, Shimosato Y, Pastan I, Shimizu N. Molecular evidence for the lack of epidermal growth factor receptor gene expression in small cell lung carcinoma cells. Cancer Res. 1987 May 15;47(10):2668–2673. [PubMed] [Google Scholar]
  • Gill GN, Bertics PJ, Santon JB. Epidermal growth factor and its receptor. Mol Cell Endocrinol. 1987 Jun;51(3):169–186. [PubMed] [Google Scholar]
  • Hendler FJ, Ozanne BW. Human squamous cell lung cancers express increased epidermal growth factor receptors. J Clin Invest. 1984 Aug;74(2):647–651. [PMC free article] [PubMed] [Google Scholar]
  • Hunter T, Cooper JA. Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells. Cell. 1981 Jun;24(3):741–752. [PubMed] [Google Scholar]
  • Hwang DL, Tay YC, Lin SS, Lev-Ran A. Expression of epidermal growth factor receptors in human lung tumors. Cancer. 1986 Nov 15;58(10):2260–2263. [PubMed] [Google Scholar]
  • Ishitoya J, Toriyama M, Oguchi N, Kitamura K, Ohshima M, Asano K, Yamamoto T. Gene amplification and overexpression of EGF receptor in squamous cell carcinomas of the head and neck. Br J Cancer. 1989 Apr;59(4):559–562. [PMC free article] [PubMed] [Google Scholar]
  • King AC, Cuatrecasas P. Resolution of high and low affinity epidermal growth factor receptors. Inhibition of high affinity component by low temperature, cycloheximide, and phorbol esters. J Biol Chem. 1982 Mar 25;257(6):3053–3060. [PubMed] [Google Scholar]
  • Libermann TA, Razon N, Bartal AD, Yarden Y, Schlessinger J, Soreq H. Expression of epidermal growth factor receptors in human brain tumors. Cancer Res. 1984 Feb;44(2):753–760. [PubMed] [Google Scholar]
  • Neal DE, Marsh C, Bennett MK, Abel PD, Hall RR, Sainsbury JR, Harris AL. Epidermal-growth-factor receptors in human bladder cancer: comparison of invasive and superficial tumours. Lancet. 1985 Feb 16;1(8425):366–368. [PubMed] [Google Scholar]
  • Nickell KA, Halper J, Moses HL. Transforming growth factors in solid human malignant neoplasms. Cancer Res. 1983 May;43(5):1966–1971. [PubMed] [Google Scholar]
  • Reynolds FH, Jr, Todaro GJ, Fryling C, Stephenson JR. Human transforming growth factors induce tyrosine phosphorylation of EGF receptors. Nature. 1981 Jul 16;292(5820):259–262. [PubMed] [Google Scholar]
  • Sainsbury JR, Farndon JR, Needham GK, Malcolm AJ, Harris AL. Epidermal-growth-factor receptor status as predictor of early recurrence of and death from breast cancer. Lancet. 1987 Jun 20;1(8547):1398–1402. [PubMed] [Google Scholar]
  • Schlessinger J. The epidermal growth factor receptor as a multifunctional allosteric protein. Biochemistry. 1988 May 3;27(9):3119–3123. [PubMed] [Google Scholar]
  • Sobol RE, Astarita RW, Hofeditz C, Masui H, Fairshter R, Royston I, Mendelsohn J. Epidermal growth factor receptor expression in human lung carcinomas defined by a monoclonal antibody. J Natl Cancer Inst. 1987 Sep;79(3):403–407. [PubMed] [Google Scholar]
  • Veale D, Ashcroft T, Marsh C, Gibson GJ, Harris AL. Epidermal growth factor receptors in non-small cell lung cancer. Br J Cancer. 1987 May;55(5):513–516. [PMC free article] [PubMed] [Google Scholar]
  • Veale D, Kerr N, Gibson GJ, Harris AL. Characterization of epidermal growth factor receptor in primary human non-small cell lung cancer. Cancer Res. 1989 Mar 1;49(5):1313–1317. [PubMed] [Google Scholar]
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