Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies.

E A Nelson; J Christopher-Hennings; T Drew; G Wensvoort; J E Collins; D A Benfield

doi:10.1128/jcm.31.12.3184-3189.1993

J Clin Microbiol. 1993 Dec; 31(12): 3184–3189.

doi: 10.1128/jcm.31.12.3184-3189.1993

PMCID: PMC266373

PMID: 7508455

Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies.

E A Nelson, J Christopher-Hennings, T Drew, G Wensvoort, J E Collins, and D A Benfield

Author information Copyright and License information PMC Disclaimer

Abstract

Monoclonal antibodies (MAbs) to two U.S. isolates of porcine reproductive and respiratory syndrome (PRRS) virus were prepared. Two MAbs specifically recognized a conserved epitope on the putative 15-kDa nucleocapsid protein of U.S. and European isolates of PRRS virus. Four other MAbs recognized epitopes on the 15-kDa protein of U.S. but not European isolates of PRRS virus. Collectively, this indicates that PRRS viruses contain both conserved and divergent epitopes on the 15-kDa viral protein.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Image
on p.3186

Image
on p.3187

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Porcine reproductive and respiratory syndrome (PRRS or blue-eared pig disease). Vet Rec. 1992 Feb 1;130(5):87–89. [PubMed] [Google Scholar]
Benfield DA, Jackwood DJ, Bac I, Saif LJ, Wesley RD. Detection of transmissible gastroenteritis virus using cDNA probes. Arch Virol. 1991;116(1-4):91–106. [PMC free article] [PubMed] [Google Scholar]
Benfield DA, Nelson E, Collins JE, Harris L, Goyal SM, Robison D, Christianson WT, Morrison RB, Gorcyca D, Chladek D. Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J Vet Diagn Invest. 1992 Apr;4(2):127–133. [PubMed] [Google Scholar]
Benfield DA, Stotz I, Moore R, McAdaragh JP. Shedding of rotavirus in feces of sows before and after farrowing. J Clin Microbiol. 1982 Jul;16(1):186–190. [PMC free article] [PubMed] [Google Scholar]
Cavanagh D, Horzinek MC. Genus Torovirus assigned to the Coronaviridae. Arch Virol. 1993;128(3-4):395–396. [PMC free article] [PubMed] [Google Scholar]
Collins JE, Benfield DA, Christianson WT, Harris L, Hennings JC, Shaw DP, Goyal SM, McCullough S, Morrison RB, Joo HS, et al. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Invest. 1992 Apr;4(2):117–126. [PubMed] [Google Scholar]
Conzelmann KK, Visser N, Van Woensel P, Thiel HJ. Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology. 1993 Mar;193(1):329–339. [PMC free article] [PubMed] [Google Scholar]
de Vries AA, Chirnside ED, Horzinek MC, Rottier PJ. Structural proteins of equine arteritis virus. J Virol. 1992 Nov;66(11):6294–6303. [PMC free article] [PubMed] [Google Scholar]
Galfre G, Howe SC, Milstein C, Butcher GW, Howard JC. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. [PubMed] [Google Scholar]
Harty JT, Plagemann PG. Formalin inactivation of the lactate dehydrogenase-elevating virus reveals a major neutralizing epitope not recognized during natural infection. J Virol. 1988 Sep;62(9):3210–3216. [PMC free article] [PubMed] [Google Scholar]
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed] [Google Scholar]
McClurkin AW, Norman JO. Studies on transmissible gastroenteritis of swine. II. Selected characteristics of a cytopathogenic virus common to five isolates from transmissible gastroenteritis. Can J Comp Med Vet Sci. 1966 Jul;30(7):190–198. [PMC free article] [PubMed] [Google Scholar]
Meulenberg JJ, Hulst MM, de Meijer EJ, Moonen PL, den Besten A, de Kluyver EP, Wensvoort G, Moormann RJ. Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology. 1993 Jan;192(1):62–72. [PMC free article] [PubMed] [Google Scholar]
Plagemann PG, Moennig V. Lactate dehydrogenase-elevating virus, equine arteritis virus, and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Adv Virus Res. 1992;41:99–192. [PMC free article] [PubMed] [Google Scholar]
Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed] [Google Scholar]
Trousdale MD, Trent DW, Shelokov A. Simian hemorrhagic fever virus: a new togavirus. Proc Soc Exp Biol Med. 1975 Dec;150(3):707–711. [PubMed] [Google Scholar]
Wensvoort G, de Kluyver EP, Luijtze EA, den Besten A, Harris L, Collins JE, Christianson WT, Chladek D. Antigenic comparison of Lelystad virus and swine infertility and respiratory syndrome (SIRS) virus. J Vet Diagn Invest. 1992 Apr;4(2):134–138. [PubMed] [Google Scholar]
Westaway EG, Brinton MA, Gaidamovich SYa, Horzinek MC, Igarashi A, Käriäinen L, Lvov DK, Porterfield JS, Russell PK, Trent DW. Togaviridae. Intervirology. 1985;24(3):125–139. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)