Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

J D Dignam; R M Lebovitz; R G Roeder

doi:10.1093/nar/11.5.1475

Nucleic Acids Res. 1983 Mar 11; 11(5): 1475–1489.

doi: 10.1093/nar/11.5.1475

PMCID: PMC325809

PMID: 6828386

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

J D Dignam, R M Lebovitz, and R G Roeder

Copyright and License information PMC Disclaimer

Abstract

We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).

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Selected References

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