Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Jul 1;33(Web Server issue):W244-8.
doi: 10.1093/nar/gki408.

The HHpred interactive server for protein homology detection and structure prediction

Johannes Söding  1 , Andreas Biegert, Andrei N Lupas
Affiliations

The HHpred interactive server for protein homology detection and structure prediction

Johannes Söding et al. Nucleic Acids Res. .

Abstract

HHpred is a fast server for remote protein homology detection and structure prediction and is the first to implement pairwise comparison of profile hidden Markov models (HMMs). It allows to search a wide choice of databases, such as the PDB, SCOP, Pfam, SMART, COGs and CDD. It accepts a single query sequence or a multiple alignment as input. Within only a few minutes it returns the search results in a user-friendly format similar to that of PSI-BLAST. Search options include local or global alignment and scoring secondary structure similarity. HHpred can produce pairwise query-template alignments, multiple alignments of the query with a set of templates selected from the search results, as well as 3D structural models that are calculated by the MODELLER software from these alignments. A detailed help facility is available. As a demonstration, we analyze the sequence of SpoVT, a transcriptional regulator from Bacillus subtilis. HHpred can be accessed at http://protevo.eb.tuebingen.mpg.de/hhpred.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Start page for the HHpred server at with part of a help window overlaid.
Figure 2
Figure 2
Search results for SpoVT from Bacillus subtilis. The summary hit list at the top shows that SpoVT consists of two domains: the N-terminal domain is very similar to AbrB (rank 1) and clearly homologous to MazE (rank 3) and the C-terminal domain is similar to GAF and PAS domains (rank 2, 4–9). In the summary hit list, column ‘Prob’ gives the probability that the hit is homologous to the query. This is the principle measure of statistical significance. In the alignments below, the sequences marked ‘Q’ (‘T’) refer to the query (template) alignment. Sequences ‘ss_pred’ and ‘ss_conf’ denote the PSI-PRED secondary structure prediction and confidence values, ‘ss_dssp’ is the secondary strcuture assigned by DSSP. Upper an lower case amino acids in the consensus sequences indicate high (≳60%) and moderate (≳40%) conservation, respectively. Symbols indicating the quality of the column–column match: ‘|’ very good, ‘+’ good, ‘·’ neutral, ‘−’ bad and ‘=’ very bad.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 1990;215:403–410. - PubMed
    1. Pearson W.R. Searching protein sequence libraries: comparison of the sensitivity and selectivity of the Smith-Waterman and FASTA algorithms. Genomics. 1991;11:635–650. - PubMed
    1. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acid Res. 1997;25:3389–3402. - PMC - PubMed
    1. Pietrokovski S. Searching databases of conserved sequence regions by aligning protein multiple-alignments. Nucleic Acids Res. 1996;24:3836–3845. - PMC - PubMed
    1. Rychlewski L., Zhang B., Godzik A. Fold and function predictions for Mycoplasma genitalium proteins. Fold Des. 1998;3:229–238. - PubMed

Publication types

-