Resequencing of 200 human exomes identifies an excess of low-frequency non-synonymous coding variants
- PMID: 20890277
- DOI: 10.1038/ng.680
Resequencing of 200 human exomes identifies an excess of low-frequency non-synonymous coding variants
Abstract
Targeted capture combined with massively parallel exome sequencing is a promising approach to identify genetic variants implicated in human traits. We report exome sequencing of 200 individuals from Denmark with targeted capture of 18,654 coding genes and sequence coverage of each individual exome at an average depth of 12-fold. On average, about 95% of the target regions were covered by at least one read. We identified 121,870 SNPs in the sample population, including 53,081 coding SNPs (cSNPs). Using a statistical method for SNP calling and an estimation of allelic frequencies based on our population data, we derived the allele frequency spectrum of cSNPs with a minor allele frequency greater than 0.02. We identified a 1.8-fold excess of deleterious, non-syonomyous cSNPs over synonymous cSNPs in the low-frequency range (minor allele frequencies between 2% and 5%). This excess was more pronounced for X-linked SNPs, suggesting that deleterious substitutions are primarily recessive.
Similar articles
-
Deep sequencing of Danish Holstein dairy cattle for variant detection and insight into potential loss-of-function variants in protein coding genes.BMC Genomics. 2015 Dec 9;16:1043. doi: 10.1186/s12864-015-2249-y. BMC Genomics. 2015. PMID: 26645365 Free PMC article.
-
Genome at juncture of early human migration: a systematic analysis of two whole genomes and thirteen exomes from Kuwaiti population subgroup of inferred Saudi Arabian tribe ancestry.PLoS One. 2014 Jun 4;9(6):e99069. doi: 10.1371/journal.pone.0099069. eCollection 2014. PLoS One. 2014. PMID: 24896259 Free PMC article.
-
Targeted capture and massively parallel sequencing of 12 human exomes.Nature. 2009 Sep 10;461(7261):272-6. doi: 10.1038/nature08250. Epub 2009 Aug 16. Nature. 2009. PMID: 19684571 Free PMC article.
-
Prediction of deleterious human alleles.Hum Mol Genet. 2001 Mar 15;10(6):591-7. doi: 10.1093/hmg/10.6.591. Hum Mol Genet. 2001. PMID: 11230178
-
Characterization of single-nucleotide polymorphisms in coding regions of human genes.Nat Genet. 1999 Jul;22(3):231-8. doi: 10.1038/10290. Nat Genet. 1999. PMID: 10391209
Cited by
-
DVA: predicting the functional impact of single nucleotide missense variants.BMC Bioinformatics. 2024 Mar 6;25(Suppl 1):100. doi: 10.1186/s12859-024-05709-6. BMC Bioinformatics. 2024. PMID: 38448823 Free PMC article.
-
A tree-based gene-environment interaction analysis with rare features.Stat Anal Data Min. 2022 Oct;15(5):648-674. doi: 10.1002/sam.11578. Epub 2022 Mar 1. Stat Anal Data Min. 2022. PMID: 38046814 Free PMC article.
-
Evolutionary history of an Alpine Archaeognath (Machilis pallida): Insights from different variant.Ecol Evol. 2023 Jul 3;13(7):e10227. doi: 10.1002/ece3.10227. eCollection 2023 Jul. Ecol Evol. 2023. PMID: 37404697 Free PMC article.
-
Viral intra-host evolutionary dynamics revealed via serial passage of Japanese encephalitis virus in vitro.Virus Evol. 2023 Mar 28;9(1):veac103. doi: 10.1093/ve/veac103. eCollection 2023. Virus Evol. 2023. PMID: 37205166 Free PMC article.
-
Relationship between BCL2 mutations and follicular lymphoma outcome in the chemoimmunotherapy era.Blood Cancer J. 2023 May 17;13(1):81. doi: 10.1038/s41408-023-00847-1. Blood Cancer J. 2023. PMID: 37193683 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources