Microbiological characterization in children with aggressive periodontitis
- PMID: 22863892
- PMCID: PMC3446830
- DOI: 10.1177/0022034512456039
Microbiological characterization in children with aggressive periodontitis
Abstract
The objective of this study was to characterize the subgingival microbiota of African-American children with Localized Aggressive Periodontitis (LAP). Fifty-one children were included. Subgingival plaque samples were taken from diseased (DD) and healthy sites (DH) in LAP and from healthy sites in HS and HC and analyzed by 16S rRNA-based microarrays. Aggregatibacter actinomycetemcomitans (Aa) was the only species found to be both more prevalent (OR = 8.3, p = 0.0025) and abundant (p < 0.01) in DD. Filifactor alocis (Fa) was also found to be more prevalent in DD (OR 2.31, CI 1.06-5.01, p = 0.03). Most prevalent species in healthy sites were Selenomonas spp, Veillonella spp, Streptococcus spp, Bergeyella sp, and Kingella oralis. Overall, Streptococcus spp, Campylobacter gracilis, Capnocytophaga granulosa, Haemophilus parainfluenzae, and Lautropia mirabilis were most abundant in healthy children, while Aa, Fa, Tannerella sp, Solobacterium moorei, Parvimonas micra, and Capnocytophaga sp were most abundant in LAP. Based on a comprehensive analysis with 16S rRNA-based microarrays, Aa was strongly associated and site-specific in LAP. In contrast, other species were found to be associated with healthy sites and individuals (ClinicalTrials.gov number CT01330719).
Abbreviations: healthy site in healthy sibling (HS); healthy site in healthy control child (HC).
Trial registration: ClinicalTrials.gov NCT01330719.
Conflict of interest statement
The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.
Figures
![Figure.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3446830/bin/10.1177_0022034512456039-fig1.gif)
Similar articles
-
Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray.J Periodontol. 2009 Sep;80(9):1421-32. doi: 10.1902/jop.2009.090185. J Periodontol. 2009. PMID: 19722792 Free PMC article.
-
Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray.J Periodontol. 2012 Oct;83(10):1279-87. doi: 10.1902/jop.2012.110566. Epub 2012 Feb 10. J Periodontol. 2012. PMID: 22324467 Free PMC article.
-
The subgingival microbiota of Papillon-Lefèvre syndrome.J Periodontol. 2012 Jul;83(7):902-8. doi: 10.1902/jop.2011.110450. Epub 2011 Dec 5. J Periodontol. 2012. PMID: 22141356
-
A consortium of Aggregatibacter actinomycetemcomitans, Streptococcus parasanguinis, and Filifactor alocis is present in sites prior to bone loss in a longitudinal study of localized aggressive periodontitis.J Clin Microbiol. 2013 Sep;51(9):2850-61. doi: 10.1128/JCM.00729-13. Epub 2013 Jun 19. J Clin Microbiol. 2013. PMID: 23784124 Free PMC article.
-
Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis.J Periodontol. 2014 Jun;85(6):819-28. doi: 10.1902/jop.2013.130306. Epub 2013 Oct 21. J Periodontol. 2014. PMID: 24144271
Cited by
-
Comparison of the main pathogenic microorganisms of various common oral diseases in children and adults.Pediatr Discov. 2023 Dec;1(3):e35. doi: 10.1002/pdi3.35. Epub 2023 Oct 5. Pediatr Discov. 2023. PMID: 38371743 Free PMC article.
-
Effect of 15 days -6° head-down bed rest on microbial communities of supragingival plaque in young men.Front Microbiol. 2024 Jan 24;15:1331023. doi: 10.3389/fmicb.2024.1331023. eCollection 2024. Front Microbiol. 2024. PMID: 38328428 Free PMC article.
-
Biomarkers for diagnosis of stage III, grade C with molar incisor pattern periodontitis in children and young adults: a systematic review and meta-analysis.Clin Oral Investig. 2023 Sep;27(9):4929-4955. doi: 10.1007/s00784-023-05169-x. Epub 2023 Aug 3. Clin Oral Investig. 2023. PMID: 37535199 Free PMC article. Review.
-
Characterization of the oral microbiome and gut microbiome of dental caries and extrinsic black stain in preschool children.Front Microbiol. 2023 Mar 31;14:1081629. doi: 10.3389/fmicb.2023.1081629. eCollection 2023. Front Microbiol. 2023. PMID: 37065128 Free PMC article.
-
Classification of salivary bacteriome in asymptomatic COVID-19 cases based on long-read nanopore sequencing.Exp Biol Med (Maywood). 2022 Nov;247(21):1937-1946. doi: 10.1177/15353702221118091. Epub 2022 Sep 8. Exp Biol Med (Maywood). 2022. PMID: 36082397 Free PMC article.
References
-
- Alaluusua S, Asikainen S. (1988). Detection and distribution of Actinobacillus actinomycetemcomitans in the primary dentition. J Periodontol 59:504-507 - PubMed
-
- Alfant B, Shaddox LM, Tobler J, Magnusson I, Aukhil I, Walker C. (2008). Matrix metalloproteinase levels in children with aggressive periodontitis. J Periodontol 79:819-826 - PubMed
-
- Armitage GC. (1999). Development of a classification system for periodontal diseases and conditions. Ann Periodontol 4:1-6 - PubMed
-
- Colombo AP, Bennet S, Cotton SL, Goodson JM, Kent R, Haffajee AD, et al. (2012). Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and “refractory” subjects by the Human Oral Microbe Identification Microarray (HOMIM). J Periodontol [epub ahead of print 2/10/2012] (in press) - PMC - PubMed
Publication types
MeSH terms
Substances
Associated data
Grants and funding
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous