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. 2015 Jun;8(6):502-5.
doi: 10.1016/j.apjtm.2015.05.008. Epub 2015 Jun 25.

Effect of microRNA-101 on apoptosis of rabbit condylar cartilage cells by inhibiting target gene SOX9

Xin Li  1 , Zi-Xin Wang  2 , Zi-Sheng Wang  3 , Quan-Fang Li  4
Affiliations
Free article

Effect of microRNA-101 on apoptosis of rabbit condylar cartilage cells by inhibiting target gene SOX9

Xin Li et al. Asian Pac J Trop Med. 2015 Jun.
Free article

Abstract

Objective: To explore the effect of microRNA-101 on apoptosis of condylar cartilage cells and the specific mechanism of molecular biology.

Methods: IL-1 was used to stimulate and establish the model of apoptosis of condylar cartilage cells. The expression change of miR-101 in control group was compared with that in IL-1 stimulation group by qRT-PCR. Overexpression and down-regulation models of miR-101 were established by transfecting Mimics and Inhibitor and verified by qRT-PCR. Flow cytometry was used to detect the effect of miR-101 overexpression and down-regulation on apoptosis. Target gene of miR-101 was analyzed and calculated through bioinformatics. Western blot and Luciferase report assay were used to detect whether SOX9 could become the target gene of miR-101.

Results: qRT-PCR results showed that IL-1 stimulation could cause the increase of miR-101 expression. After the transfection of rabbit condylar cartilage cells by Mimics and Inhibitor, qRT-PCR results confirmed the significant effect of miR-101 overexpression and down-regulation. It was confirmed by flow cytometry that overexpression of miR-101 could promote the apoptosis of condylar cartilage cells, and down-regulation of miR-101 could reduce the apoptosis. It was confirmed by Western blot and Luciferase report assay that SOX9 was the target gene of miR-101, and miR-101 inhibited SOX9 expression through complementary pairing with 3'UTR of SOX9 mRNA.

Conclusions: miR-101 can promote the apoptosis of condylar cartilage cells through inhibiting the protein level of target gene SOX9.

Keywords: Apoptosis; Condyle cartilage; SOX9; miR-101.

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