Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes
- PMID: 27159373
- PMCID: PMC4900911
- DOI: 10.1038/nbt.3567
Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes
Abstract
We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We found that the precision of the two libraries in detecting essential genes was similar and that combining data from both screens improved performance. Notably, results from the two screens showed little correlation, which can be partially explained by the identification of distinct essential biological processes with each technology.
Conflict of interest statement
Competing Financial Interests
The authors declare no competing financial interests.
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Comment in
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Comparing CRISPR and RNAi-based screening technologies.Nat Biotechnol. 2016 Jun 9;34(6):621-3. doi: 10.1038/nbt.3599. Nat Biotechnol. 2016. PMID: 27281421 No abstract available.
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