Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Apr 9;13(4):e0195753.
doi: 10.1371/journal.pone.0195753. eCollection 2018.

Green tea extracts ameliorate high-fat diet-induced muscle atrophy in senescence-accelerated mouse prone-8 mice

Shintaro Onishi  1 , Mayu Ishino  2 , Hidefumi Kitazawa  1 , Ai Yoto  1 , Yuki Shimba  2 , Yusuke Mochizuki  2 , Keiko Unno  3 , Shinichi Meguro  1 , Ichiro Tokimitsu  1 , Shinji Miura  2
Affiliations

Green tea extracts ameliorate high-fat diet-induced muscle atrophy in senescence-accelerated mouse prone-8 mice

Shintaro Onishi et al. PLoS One. .

Abstract

Muscle atrophy (loss of skeletal muscle mass) causes progressive deterioration of skeletal function. Recently, excessive intake of fats was suggested to induce insulin resistance, followed by muscle atrophy. Green tea extracts (GTEs), which contain polyphenols such as epigallocatechin gallate, have beneficial effects on obesity, hyperglycemia, and insulin resistance, but their effects against muscle atrophy are still unclear. Here, we found that GTEs prevented high-fat (HF) diet-induced muscle weight loss in senescence-accelerated mouse prone-8 (SAMP8), a murine model of senescence. SAMP8 mice were fed a control diet, an HF diet, or HF with 0.5% GTEs (HFGT) diet for 4 months. The HF diet induced muscle weight loss with aging (measured as quadriceps muscle weight), whereas GTEs prevented this loss. In HF diet-fed mice, blood glucose and plasma insulin concentrations increased in comparison with the control group, and these mice had insulin resistance as determined by homeostasis model assessment of insulin resistance (HOMA-IR). In these mice, serum concentrations of leukocyte cell-derived chemotaxin 2 (LECT2), which is known to induce insulin resistance in skeletal muscle, were elevated, and insulin signaling in muscle, as determined by the phosphorylation levels of Akt and p70 S6 kinases, tended to be decreased. In HFGT diet-fed mice, these signs of insulin resistance and elevation of serum LECT2 were not observed. Although our study did not directly show the effect of serum LECT2 on muscle weight, insulin resistance examined using HOMA-IR indicated an intervention effect of serum LECT2 on muscle weight, as revealed by partial correlation analysis. Accordingly, GTEs might have beneficial effects on age-related and HF diet-induced muscle weight loss, which correlates with insulin resistance and is accompanied by a change in serum LECT2.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have the following interests: This work was supported by industry-academia collaboration between the University of Shizuoka and Kao Corporation. There are no patents, products in development or marketed products to declare. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Figures

Fig 1
Fig 1. Body weight and skeletal muscle weight in aging SAMR1 and SAMP8 mice.
Body weight (A) and skeletal muscle weight (B) were measured in young (2M) and adult (6M) mice fed a Cont diet. Skeletal muscle weight increased in SAMR1 mice concomitantly with body weight gain, but did not increase in SAMP8 mice. Data are means ± S.D. (8 to 16 mice per group). Statistical significance of the interaction between mouse strain (SAMR1 and SAMP8) and age (2M and 6M) was determined by two-way factorial ANOVA without replication, and Student’s t-test was used for comparison between the two age groups. *, P < 0.05; ***, P < 0.001.
Fig 2
Fig 2. Body weight and skeletal muscle weight in SAMP8 mice on different diets.
Body weight (A); skeletal muscle weight (B). Skeletal muscle loss with aging was exacerbated by an HF diet (2M Cont vs. 6M HF; P = 0.016) but was significantly prevented by GTEs in the HFGT group (HF vs. HFGT; P = 0.002). Data are means ± S.D. (8 to 16 mice per group). One-way ANOVA followed by Tukey’s post-hoc test was used for comparison among groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Fig 3
Fig 3. Blood glucose, plasma insulin, HOMA-IR, and serum LECT2 concentrations in SAMP8 mice.
Blood glucose levels were analyzed at dissection after 5 h of fasting by ACCU-CHEK Aviva described in Materials and Methods (A). Plasma insulin (B) and serum LECT2 (D) concentrations were analyzed after serum and plasma sample collection by enzyme-linked immuno-sorbent assay. HOMA-IR was calculated as described in the Materials and Methods (C). Data are means ± S.D. One-way ANOVA followed by Tukey’s post-hoc test was used for comparison among groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Fig 4
Fig 4. Phosphorylation of kinases involved in insulin signaling in skeletal muscle of adult (6M) SAMP8 mice.
Representative western blot images (upper panels, all images provided in S1 Appendix) and quantification of phosphorylation (lower graphs) are shown for Akt (A) and S6K (B). Data are means ± S.D. One-way ANOVA followed by Tukey’s post-hoc test was used for comparison among groups. *, P < 0.05.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Hairi NN, Cumming RG, Naganathan V, Handelsman DJ, Le Couteur DG, Creasey H, et al. Loss of muscle strength, mass (sarcopenia), and quality (specific force) and its relationship with functional limitation and physical disability: the Concord Health and Ageing in Men Project. J Am Geriatr Soc. 2010;58(11):2055–62. Epub 2010/11/09. doi: 10.1111/j.1532-5415.2010.03145.x . - DOI - PubMed
    1. McGregor RA, Cameron-Smith D, Poppitt SD. It is not just muscle mass: a review of muscle quality, composition and metabolism during ageing as determinants of muscle function and mobility in later life. Longev Healthspan. 2014;3(1):9 Epub 2014/12/19. doi: 10.1186/2046-2395-3-9 - DOI - PMC - PubMed
    1. Keller K, Engelhardt M. Strength and muscle mass loss with aging process. Age and strength loss. Muscles Ligaments Tendons J. 2013;3(4):346–50. Epub 2014/03/07. - PMC - PubMed
    1. Brady AO, Straight CR. Muscle capacity and physical function in older women: What are the impacts of resistance training? Journal of Sport and Health Science. 2014;3(3):179–88. https://doi.org/10.1016/j.jshs.2014.04.002. - DOI
    1. Kim HK, Suzuki T, Saito K, Yoshida H, Kobayashi H, Kato H, et al. Effects of exercise and amino acid supplementation on body composition and physical function in community-dwelling elderly Japanese sarcopenic women: a randomized controlled trial. J Am Geriatr Soc. 2012;60(1):16–23. Epub 2011/12/07. doi: 10.1111/j.1532-5415.2011.03776.x . - DOI - PubMed

Publication types

Grants and funding

This work was supported by industry-academia collaboration between the University of Shizuoka and Kao Corporation. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
-