An electrophoretic karyotype of Neurospora crassa

doi:10.1128/mcb.8.4.1469-1473.1988

. 1988 Apr;8(4):1469-73.

doi: 10.1128/mcb.8.4.1469-1473.1988.

An electrophoretic karyotype of Neurospora crassa

M J Orbach¹, D Vollrath, R W Davis, C Yanofsky

Affiliations

PMID: 2967910
PMCID: PMC363304
DOI: 10.1128/mcb.8.4.1469-1473.1988

An electrophoretic karyotype of Neurospora crassa

M J Orbach et al. Mol Cell Biol. 1988 Apr.

. 1988 Apr;8(4):1469-73.

doi: 10.1128/mcb.8.4.1469-1473.1988.

Authors

M J Orbach¹, D Vollrath, R W Davis, C Yanofsky

Affiliation

¹ Department of Biological Sciences, Stanford University, California 94305-5020.

PMID: 2967910
PMCID: PMC363304
DOI: 10.1128/mcb.8.4.1469-1473.1988

Abstract

A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.

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References

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1. Mol Cell Biol. 1986 Jul;6(7):2452-61 - PubMed
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[1] Cell. 1984 May;37(1):67-75 - PubMed

[2] Cell. 1984 May;37(1):67-75 - PubMed

[3] Mol Cell Biol. 1986 Jul;6(7):2452-61 - PubMed

[4] Mol Cell Biol. 1986 Jul;6(7):2452-61 - PubMed

[5] Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed

[6] Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed

[7] Mol Gen Genet. 1983;190(3):403-5 - PubMed

[8] Mol Gen Genet. 1983;190(3):403-5 - PubMed

[9] Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756-60 - PubMed

[10] Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756-60 - PubMed

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An electrophoretic karyotype of Neurospora crassa

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An electrophoretic karyotype of Neurospora crassa

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