Production of 'hybrid' antibiotics by genetic engineering
- PMID: 3857464
- DOI: 10.1038/314642a0
Production of 'hybrid' antibiotics by genetic engineering
Abstract
The recent development of molecular cloning systems in Streptomyces has made possible the isolation of biosynthetic genes for some of the many antibiotics produced by members of this important genus of bacteria. Such clones can now be used to test the idea that novel antibiotics could arise through the transfer of biosynthetic genes between streptomycetes producing different antibiotics. The likelihood of a 'hybrid' compound being produced must depend on the substrate specificities of the biosynthetic enzymes, about which little is known. In attempts to demonstrate hybrid antibiotic production, we therefore began with strains producing different members of the same chemical class of compounds in order to maximize the chance of success. Here we report the production of novel compounds by gene transfer between strains producing the isochromanequinone antibiotics actinorhodin, granaticin and medermycin. These experiments were made possible by the recent cloning of the whole set of genes for the biosynthetic pathway of actinorhodin from Streptomyces coelicolor A3(2) (ref. 8). We believe that this represents the first report of the production of hybrid antibiotics by genetic engineering.
Similar articles
-
Biosynthetic conclusions from the functional dissection of oxygenases for biosynthesis of actinorhodin and related Streptomyces antibiotics.Chem Biol. 2013 Apr 18;20(4):510-20. doi: 10.1016/j.chembiol.2013.03.007. Chem Biol. 2013. PMID: 23601640
-
Functional complementation of pyran ring formation in actinorhodin biosynthesis in Streptomyces coelicolor A3(2) by ketoreductase genes for granaticin biosynthesis.J Bacteriol. 2001 May;183(10):3247-50. doi: 10.1128/JB.183.10.3247-3250.2001. J Bacteriol. 2001. PMID: 11325954 Free PMC article.
-
Genetic manipulation of antibiotic-producing Streptomyces.Trends Microbiol. 1998 Feb;6(2):76-83. doi: 10.1016/S0966-842X(97)01161-X. Trends Microbiol. 1998. PMID: 9507643 Review.
-
Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host.Nature. 1984 May 31-Jun 6;309(5967):462-4. doi: 10.1038/309462a0. Nature. 1984. PMID: 6328317
-
Significance of anthraquinone formation resulting from the cloning of actinorhodin genes in heterologous streptomycetes.Mol Microbiol. 1992 Jan;6(2):147-52. doi: 10.1111/j.1365-2958.1992.tb01995.x. Mol Microbiol. 1992. PMID: 1545701 Review.
Cited by
-
Strategies on biosynthesis and production of bioactive compounds in medicinal plants.Chin Herb Med. 2023 Aug 21;16(1):13-26. doi: 10.1016/j.chmed.2023.01.007. eCollection 2024 Jan. Chin Herb Med. 2023. PMID: 38375043 Free PMC article. Review.
-
The Best of Both Worlds-Streptomyces coelicolor and Streptomyces venezuelae as Model Species for Studying Antibiotic Production and Bacterial Multicellular Development.J Bacteriol. 2023 Jul 25;205(7):e0015323. doi: 10.1128/jb.00153-23. Epub 2023 Jun 22. J Bacteriol. 2023. PMID: 37347176 Free PMC article. Review.
-
Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites.Microorganisms. 2023 Apr 27;11(5):1141. doi: 10.3390/microorganisms11051141. Microorganisms. 2023. PMID: 37317115 Free PMC article. Review.
-
Bioengineered Probiotics: Synthetic Biology Can Provide Live Cell Therapeutics for the Treatment of Foodborne Diseases.Front Bioeng Biotechnol. 2022 May 17;10:890479. doi: 10.3389/fbioe.2022.890479. eCollection 2022. Front Bioeng Biotechnol. 2022. PMID: 35656199 Free PMC article. Review.
-
Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast.Synth Syst Biotechnol. 2020 Jan 23;5(1):11-18. doi: 10.1016/j.synbio.2020.01.004. eCollection 2020 Mar. Synth Syst Biotechnol. 2020. PMID: 32021916 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases