Functional comparison of D-serine and glycine in rodents: the effect on cloned NMDA receptors and the extracellular concentration
- PMID: 7790891
- DOI: 10.1046/j.1471-4159.1995.65010454.x
Functional comparison of D-serine and glycine in rodents: the effect on cloned NMDA receptors and the extracellular concentration
Abstract
We compared the activity of free D-Ser on the potentiation of cloned NMDA receptors with that of Gly by using a Xenopus oocyte expression system. The extracellular concentration of free D-Ser and Gly was further studied by means of microdialysis. The ED50 values of D-Ser were three to four times lower than those of Gly in any combination of epsilon 1, epsilon 2, epsilon 3, or epsilon 4 and zeta 1. Site-directed mutagenesis of zeta 1 subunits revealed that some aromatic residues necessary for the action of Gly affected the ED50 value of D-Ser. This result showed that the residues play crucial roles in the action of D-Ser. In vivo microdialysis of rodent brain revealed that the extracellular concentration of free D-Ser in the frontal cortex (6.5 microM) was high enough to saturate the Gly site on the NMDA receptor, but that in the cerebellum was not. These findings suggest that D-Ser is a candidate of the endogenous potentiator of the NMDA receptor in the rodent frontal cortex.
Similar articles
-
Contribution of endogenous glycine and d-serine to excitotoxic and ischemic cell death in rat cerebrocortical slice cultures.Life Sci. 2007 Aug 9;81(9):740-9. doi: 10.1016/j.lfs.2007.07.001. Epub 2007 Jul 18. Life Sci. 2007. PMID: 17698151
-
Differential ethanol sensitivity of recombinant N-methyl-D-aspartate receptor subunits.Mol Pharmacol. 1994 Feb;45(2):324-9. Mol Pharmacol. 1994. PMID: 8114679
-
Interference with cellular differentiation by D-serine through antagonism at N-methyl-D-aspartate receptors composed of NR1 and NR3A subunits in chondrocytes.J Cell Physiol. 2009 Sep;220(3):756-64. doi: 10.1002/jcp.21821. J Cell Physiol. 2009. PMID: 19452450
-
[Development of a novel pharmacotherapy targeted at the N-methyl-D-aspartate receptor-D-serine system for schizophrenia].Nihon Shinkei Seishin Yakurigaku Zasshi. 2010 Nov;30(5-6):201-6. Nihon Shinkei Seishin Yakurigaku Zasshi. 2010. PMID: 21226316 Review. Japanese.
-
Allosteric modulation of NMDA receptor via elevation of brain glycine and D-serine: the therapeutic potentials for schizophrenia.Pharmacol Ther. 2008 Dec;120(3):317-32. doi: 10.1016/j.pharmthera.2008.08.004. Epub 2008 Aug 27. Pharmacol Ther. 2008. PMID: 18805436 Review.
Cited by
-
Cross species review of the physiological role of D-serine in translationally relevant behaviors.Amino Acids. 2023 Nov;55(11):1501-1517. doi: 10.1007/s00726-023-03338-6. Epub 2023 Oct 13. Amino Acids. 2023. PMID: 37833512 Free PMC article. Review.
-
Luvadaxistat: A Novel Potent and Selective D-Amino Acid Oxidase Inhibitor Improves Cognitive and Social Deficits in Rodent Models for Schizophrenia.Neurochem Res. 2023 Oct;48(10):3027-3041. doi: 10.1007/s11064-023-03956-2. Epub 2023 Jun 8. Neurochem Res. 2023. PMID: 37289348 Free PMC article.
-
Blockade of D-serine signaling and adult hippocampal neurogenesis attenuates remote contextual fear memory following multiple memory retrievals in male mice.Front Neurosci. 2023 Jan 4;16:1030702. doi: 10.3389/fnins.2022.1030702. eCollection 2022. Front Neurosci. 2023. PMID: 36685240 Free PMC article.
-
Excitatory and inhibitory D-serine binding to the NMDA receptor.Elife. 2022 Oct 27;11:e77645. doi: 10.7554/eLife.77645. Elife. 2022. PMID: 36301074 Free PMC article.
-
Promising Application of D-Amino Acids toward Clinical Therapy.Int J Mol Sci. 2022 Sep 16;23(18):10794. doi: 10.3390/ijms231810794. Int J Mol Sci. 2022. PMID: 36142706 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources