biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:408-414, 2009 | DOI: 10.1007/s10535-009-0079-y

Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation

D. S. Kim1,*, S. Y. Kim1, I. Y. Jeong1, J. -B. Kim1, G. -J. Lee1, S. -Y. Kang1, W. Kim2
1 Radiation Research Center for Bio-technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeonbuk, Republic of Korea
2 Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea

In order to evaluate effects of γ-rays on adventitious root formation and ginsenoside production, embryogenic calli induced from cotyledon explants of Panax ginseng C.A. Meyer were treated with γ-rays of 0, 10, 30, 50, 70, and 100 Gy. The highest frequency of adventitious root formation of 75 % occurred at γ-irradiation of 30 Gy, which is considered adequate dosage for selecting mutant cell lines. Five mutated adventitious roots (MAR)3-lines out of the propagation of 142 adventitious root lines treated with 30 Gy were selected based a 100-fold increase in proliferation rate compared to control adventitious roots (CAR) and content of the seven major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) was determined. In the CAR and four of the MAR3-lines (except for MAR3-109), the Rb/Rg ratio was greater than 1.0, thereby indicating altered ginsenoside composition in these root lines. The HPLC analysis of the MAR3-13 and MAR3-26 lines confirmed different ginsenoside profiles, including the three unidentified ginsenoside candidates, Gm1, Gm2, and Gm3. The ginsenosides of the MAR3-13 and MAR3-26 lines showed high hydroxyl and superoxide radical scavenging activities.

Keywords: HPLC; mutation; radical scavenging activities
Subjects: auxins; gamma radiation; ginsenoside; in vitro culture, callus; in vitro culture, rooting; mutagenesis, mutants; Panax ginseng; radical scavenging

Received: June 14, 2007; Accepted: April 16, 2008; Published: September 1, 2009  Show citation

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Kim, D.S., Kim, S.Y., Jeong, I.Y., Kim, J.-B., Lee, G.-J., Kang, S.-Y., & Kim, W. (2009). Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation. Biologia plantarum, 53(3), 408-414. doi: 10.1007/s10535-009-0079-y
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