biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 53:435-443, 2009 | DOI: 10.1007/s10535-009-0083-2

Molecular and cytological characterization of ZTL in Ipomoea nil

A. Zienkiewicz1,*, D. J. Smoliñski2, K. Zienkiewicz2, P. Glaziñska1, W. Wojciechowski1, J. Kopcewicz1
1 Department of Plant Physiology and Molecular Biology of Plants, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruñ, Poland
2 Department of Cell Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruñ, Poland

The ZEITLUPE (ZTL) protein is involved in the control of circadian period, hypocotyl elongation and flowering time in Arabidopsis thaliana. The aim of the present work was the identification of the InZTL gene and localization of its mRNA in the model short-day plant Ipomoea nil. The deduced InZTL protein of 622 amino acid residues contained a LOV domain at the N-terminal part, followed by an F-box domain and six carboxy terminal kelch repeats. Amino acid sequence of InZTL showed 84 % homology with Mesembryanthemum crystallinum ZTL (McZTL) and 83 % with Arabidopsis thaliana ZTL (AtZTL). Fluorescence in situ hybridization (FISH) to InZTL mRNA showed its high accumulation in the vascular bundles as well in the guard cells of the cotyledon. Immunolocalization of ZTL protein indicated a similar distribution pattern of ZTL protein as InZTL mRNAs.

Keywords: fluorescence in situ hybridization (FISH); short-day plant; circadian clock
Subjects: Arabidopsis thaliana; circadian clock; fluorescence in situ hybridization; Ipomoea nil; photoperid; polymerase chain reaction (PCR); ZTL protein

Received: September 20, 2007; Accepted: April 17, 2008; Published: September 1, 2009  Show citation

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Zienkiewicz, A., Smoliñski, D.J., Zienkiewicz, K., Glaziñska, P., Wojciechowski, W., & Kopcewicz, J. (2009). Molecular and cytological characterization of ZTL in Ipomoea nil. Biologia plantarum, 53(3), 435-443. doi: 10.1007/s10535-009-0083-2
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    References

    1. Abd-El Baki, G.K., Siefritz, F., Man, H.M., Weiner, H., Kladenhoff, R., Kaiser, W.M.: Nitrate reductase in Zea mays under salinity. - Plant Cell Environ. 23: 515-521, 2000. Go to original source...
    2. Adams, J., Kelso, R., Cooley, L.: The kelch repeat super-family of proteins: propellers of cell function. - Trends Cell Biol. 10: 17-24, 2000. Go to original source...
    3. Boxall, S.F., Foster, J.M., Bohnert, J.M., Cushman, H.J., Nimmo, H.G., Hartwell, J.: Conservation and divergence of circadian clock operation in a stress-inducible Crassulacean acid metabolism species reveals clock compensation against stress. - Plant Physiol. 137: 969-82, 2005. Go to original source...
    4. Briggs, W.R., Christie, J.M.: Phototropins 1 and 2: versatile plant blue-light receptors. - Trends Plant Sci. 7: 204-210, 2002. Go to original source...
    5. Corlett, J.E., Wilkinson, S., Thompson, A.J.: Diurnal control of the drought-inducible putative histone H1 gene in tomato. - J. exp. Bot. 49: 945-952, 1998. Go to original source...
    6. Doyle, M.R., Davis, S.J., Bastow, R.M., McWatters, H.G., Kozma-Bognar, L., Nagy, F., Millar, A.J., Amasion, R.M.: The ELF4 gene controls circadian rhythms and flowering time in Arabidopsis thaliana. - Nature 419: 74-77, 2002. Go to original source...
    7. Dunlap, J.C.: Molecular bases of circadian clocks. - Cell 96: 271-290, 1999. Go to original source...
    8. Endo, M., Mochizuki, N., Suzuki, T., Nagatani, A.: CRYPTOCHROME2 in vascular bundles regulates flowering in Arabidopsis. - Plant Cell 19: 84-93, 2007. Go to original source...
    9. Endo, M., Nakamura, S., Araki, T., Mochizuki, N., Nagatani, A.: Phytochrome B in the mesophyll delays flowering by suppressing FLOWERING LOCUS T expression in Arabidopsis vascular bundles. - Plant Cell 17: 1941-1952, 2005. Go to original source...
    10. Fankhauser, C., Staiger, D.: Photoreceptors in Arabidopsis thaliana: light perception, signal transduction and entrainment of the endogenous clock. - Planta 216: 1-16, 2002. Go to original source...
    11. Fukamatsu, Y., Mitsui, S., Yasuhara, M., Tokioka, Y., Ihara, N., Fujita, S., Kiyosue, T.: Identification of LOV KELCH PROTEIN2 (LKP2)-interacting factors that can recruit LKP2 to nuclear bodies. - Plant Cell Physiol. 46: 1340-1349, 2005. Go to original source...
    12. Gardner, M.J., Hubbard, K.E., Hotta, C.T., Dodd, A.N., Webb, A.A.: How plants tell the time. - Biochem. J. 397: 1-24, 2006. Go to original source...
    13. Gorton, H.L., Williams, W.E., Binns, M.E., Gemmell, C.N., Leheny, E.A., Shepherd, C.A.: Circadian stomatal rhythms in epidermal peels from Vicia faba. - Plant Physiol. 90: 1329-1334, 1989. Go to original source...
    14. Hall, A., Kozma-Bognar, L., Toth, R., Nagy, F., Millar, A.J.: Conditional circadian regulation of PHYTOCHROME A gene expression. - Plant Physiol. 127: 1808-1818, 2001. Go to original source...
    15. Kiba, T., Henriques, R., Sakakibara, H., Chua, N.H.: Targeted degradation of PSEUDO-RESPONSE REGULATOR5 by a SCFZTL complex regulates clock function and photomorphogenesis in Arabidopsis thaliana. - Plant Cell 19: 2516-2530, 2007. Go to original source...
    16. Kim, W.Y., Fujiwara, S., Suh, S.S., Kim, J., Kim, Y., Han, L., David, K., Putterill, J., Nam, H.G., Somers, D.E.: ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light. - Nature 449: 356-360, 2007. Go to original source...
    17. Kim, W.Y., Hicks, K.A., Somers, D.E.: Independent roles for Early Flowering 3 and ZEITLUPE in the control of circadian timing, hypocotyl elongation and flowering time. - Plant Physiol. 139: 1557-1569, 2005. Go to original source...
    18. Kinoshita, T., Shimazaki K.-I.: Biochemical evidence for the requirement of 14-3-3 protein binding in activation of the guard - cell plasma membrane H+-ATPase by blue light. - Plant Cell Physiol. 43: 1359-1365, 2002. Go to original source...
    19. Kiyosue, T., Wada, M.: LKP1 (LOV kelch protein 1): a factor involved in the regulation of flowering time in Arabidopsis. - Plant J. 23: 807-815, 2000. Go to original source...
    20. Mas, P., Kim, W.Y., Somers, D.E., Kay, S.A.: Targeted degradation of TOC1 by ZTL modulates circadian function in Arabidopsis thaliana. - Nature 426: 567-570, 2003. Go to original source...
    21. Oda, A., Sakuta, C., Masuda, S., Mizoguchi, T., Kamada, H., Satoh, S.: Possible involvement of leaf gibberelins in the clock-controlled expression of XP30, a gene encoding a XYLEM SAP LECTIN, in cucumber roots. - Plant Physiol. 133: 1779-1790, 2003. Go to original source...
    22. Salome, P.A., McClung, C.R.: The Arabidopsis thaliana clock. - J. biol. Rhythms 19: 425-435, 2004. Go to original source...
    23. Schultz, T.F., Kiyosue, T., Yanovsky, M., Wada, M., Kay, S.A.: A role for LKP2 in the circadian clock of Arabidopsis. - Plant Cell 13: 2659-2670, 2001. Go to original source...
    24. Somers, D.E.: The physiology and molecular bases of the plant circadian clock. - Plant Physiol. 121: 9-19, 1999. Go to original source...
    25. Somers, D.E., Devlin, P.F., Kay, S.A.: Phytochromes and cryptochromes in the entrainment of Arabidopsis circadian clock. - Science 282: 1488-1490, 1998. Go to original source...
    26. Somers, D.E, Kim, W.Y, Geng, R.: The F-box protein ZEITLUPE confers dosage-dependent control on the circadian clock, photomorphogenesis, and flowering time. - Plant Cell 16: 769-782, 2004. Go to original source...
    27. Somers, D.E., Schultz, T.F., Milnamow, M., Kay, S.A.: ZEITLUPE encodes a novel clock-associated PAS protein from Arabidopsis. - Cell 101: 319-329, 2000. Go to original source...
    28. Takada, S., Goto, K.: TERMINAL FLOWER2, an Arabidopsis homolog of HETEROCHROMATIN PROTEIN1, counteracts the activation of FLOWERING LOCUS T by CONSTANS in the vascular tissues of leaves to regulate flowering time. - Plant Cell 15: 2856-2865, 2003. Go to original source...
    29. Yasuhara, M., Mitsui, S., Hirano, H., Takanabe, R., Tokioka, Y., Ihara, N., Komatsu, A., Seki, M., Shinozaki, K., Kiyosue, T.: Identification of ASK clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis. - J. exp. Bot. 55: 2015-2027, 2004. Go to original source...
    30. Zeiger, E., Talbott, L.D., Frechilla, S., Srivastava, A., Zhu, J.X.: The guard cell chloroplast: a perspective for the twenty-first century. - New Phytol. 153: 415-424, 2002. Go to original source...

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