biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 55:253-260, 2011 | DOI: 10.1007/s10535-011-0036-4

Responses of transgenic Nicotiana tabacum seedlings expressing a Cucurbita pepo antisense PHYA RNA to far-red radiation

T. A. Gapeeva1,*, T. V. Antsipava1, A. N. Pundik1, I. D. Volotovski1
1 Institute of Biophysics and Cellular Engineering, National Academy of Sciences of Belarus, Minsk, Belarus

The Nicotiana tabacum transgenic plants expressing a Cucurbita pepo antisense PHYA RNA were obtained. The seedlings of transgenic tobacco with reduced phytochrome A (PHYA) content displayed decreased sensitivity to continuous broad-band far-red radiation (λ > 680 nm). Under far-red irradiance transgenic seedlings showed less elongation of the hypocotyls, more rapid plastid development, more chlorophyll accumulation, less repression of lightdependent NADPH:protochlorophyllide oxidoreductase than wild-type plants that was in accordance with PHYA control of plant development. Dynamics of the far-red radiation dependent changes in low temperature chlorophyll fluorescence spectra for the transgenic and wild-type seedlings were consistent with the more rapid formation of photosynthetic apparatus in the seedlings with reduced PHYA.

Keywords: antisense expression; chlorophyll; chloroplast; phytochrome-deficient tobacco; high-irradiance response

Received: August 8, 2009; Accepted: June 15, 2010; Published: June 1, 2011  Show citation

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Gapeeva, T.A., Antsipava, T.V., Pundik, A.N., & Volotovski, I.D. (2011). Responses of transgenic Nicotiana tabacum seedlings expressing a Cucurbita pepo antisense PHYA RNA to far-red radiation. Biologia plantarum, 55(2), 253-260. doi: 10.1007/s10535-011-0036-4
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