Linkage of the Rf₁ gene in the T cytoplasm of maize with genes of the 3rd chromosome

Jan Vo¾da

Biologia plantarum 8:411-420, 1966 | DOI: 10.1007/BF02930934  

The paper presents results obtained from studying the linkages of the Rf₁ gene causing the restoration of fertility of pollen in the T type of cytoplasmic sterility of maize with signal genes of the 3rd chromosome in the F₂ and B₁ hybrid generations. Crossing of the fertility-restorer lines obtained on the basis of T cytoplasm with signal lines carrying recessive genes of the 3rd linkage group was carried out during the coupling phase.The data obtained on the linkage of genes indicates that the Rf₁ gene is located at the proximal end of the long arm of the 3rd chromosome between loci d₁ and...

Vessel member length-diameter and perforation plate-vessel member length relations inDioscorea alata L.

J. J. Shah, K. Unnikrishnan, K. V. Poulose

Biologia plantarum 8:421, 1966 | DOI: 10.1007/BF02930935  

The vessel member length-diameter and perforation plate-vessel member length relations inDioscorea alata L. have been investigated. The two types of vessel elements, one with scalariform perforation plate and the other with foraminate perforation plate, have been considered for correlation tests; the variables being vessel member length and diameter, and vessel member length and perforation plate length. Significant positive correlation was found only in the case of perforation plate length and vessel member length for types with scalariform perforation plates.

Position and extent of the elongation zone in the root tip of the broad beanVicia faba L.

Alena Adámková, Karel Bene¹

Biologia plantarum 8:427, 1966 | DOI: 10.1007/BF02930936  

It was found by measuring the length of the cortex cells of the root tips of the broad beanVicia faba L. that the beginning of the elongation zone lies at about 1-2 mm from the initials and its end at about 7-8 mm from the initials. Shrinkage of the object during microtechnical treatment was negligible. The autonomy of the individual tissues of the root tip was taken into account.

Anchusa-Mosaic caused by TMV-infection in nature

Zdenko Polák

Biologia plantarum 8:431-433, 1966 | DOI: 10.1007/BF02930937  

Mosaic disease ofAnchusa officinalis found at a ruderal association in Prague-Hole¹ovice in the close proximity of TMV infected cruciferous weedsCardaria draba andSisymbrium loeselii, was identified to be caused by the same agent-the necrotic TMV-strain.

Investigation of the X virus inhibitor from potato leaf sap

Ev¾en Jermoljev, Liana Albrechtová

Biologia plantarum 8:434-443, 1966 | DOI: 10.1007/BF02930938  

The inhibitor of the X virus (Jermoljev, Albrechtová 1965) can be separated from other proteins by 60 min centrifugation at 40000 r.p.m. in the Spinco L centrifuge and by subsequent gel filtration of the supernatant on a Sephadex G-50 column. For the elution it is best to use Tris-maleate buffers and 0.2M Tris-HCl of pH 7.2. The inhibitor is present in the first fractions containing proteins, between 30 and 45 ml. It is most suitable to concentrate the inhibitor by salting out with ammonium sulphate to 30-50% saturation. The inhibitor can be preserved by adding ammonium sulphate to the sap. At 3°C it can be kept in this manner for 2 months. If the...

Serological determination of the amount of the X virus inhibitor in potato leaf sap

Ev¾en Jermoljev, Liana Albrechtová

Biologia plantarum 8:444-451, 1966 | DOI: 10.1007/BF02930939  

Antiserum against the X virus inhibitor was prepared by immunizing rabbits with the inhibitor antigen obtained in two ways: (1) ultracentrifugation of potato leaf sap in a Spinco L for 1 h at 40000 r.p.m., (2) ultracentrifugation and fractionation of the sap on a Sephadex G-50 column.The antiserum is specific against the inhibitor protein and does not react with other proteins of the plant or with the X virus. Direct serological analysis makes it possible to determine in the potato leaf sap either the presence or the absence of the X virus inhibitor, to follow the degree of inactivation of the inhibitor due to various factors and finally to determine...

Histochemical demonstration of non-specific esterase in wheat root

Jaroslava Havránková

Biologia plantarum 8:452, 1966 | DOI: 10.1007/BF02930940  

Azo-coupling methods were used for demonstrating non-specific esterase in the wheat root in all parts of a transverse section, usually with the exception of the woody parts of the vascular bundle. The central cylinder gave a more intense reaction than the primary cortex and the rhizoderm. The reaction was not inhibited by dodecyl sulphate. A weakening of the reaction intensity was observed after application of AgNO₃. The Tween method did not yield reliable results.

Studium autopathischer und allelopathischer Eigenschaften von Kulturpflanzen durch Folgekulturen-Methode

Miroslava Vicherková, Franti¹ek Plhák

Biologia plantarum 8:456-469, 1966 | DOI: 10.1007/BF02930941  

In der Arbeit wurde der Einfluss der Vorkulturen von Lein, Korn, Mohn und Ackersenf auf die folgenden Kulturen der gleichen oder einer anderen Art bei unmittelbar aufeinanderfolgenden Aussaaten sowie bei Aussaaten nach verschieden langen Intervallen unter Abstufung der Dauer der Lagerung des Bodensubstrates untersucht. Die Versuche wurden in Gefässen mit Komposterde durchgeführt, die im Versuchsgarten aufgestellt worden waren. Es wurde das Wachstum der Erst- und der Folgekultur mit Hilfe der Bestimmung der Trockensubstanz der oberirdischen Teile und der Wurzeln gewertet. Im Laufe der Wachstumsentwicklung der Folgepflanzen wurden Bodenproben entnommen...

The effect of amputation of the epicotyl on the level of endogenous gibberellins in the roots of pea seedlings

Jiøí ©ebánek

Biologia plantarum 8:470, 1966 | DOI: 10.1007/BF02930942  

The content of endogenous gibberellins was determined chromatographically in the roots 14-17 days old pea seedlings cultivated in water cultures in the dark. When the epicotyls are amputated from these plants, the content of endogenous gibberellins increases significantly within 6-12 hours after amputation as compared with the intact controls, then it falls off again considerably up to 24 hours after amputation. The initial increase of the gibberellin level in the roots can be explained by transport inhibition of the endogenous gibberellins from the root to the epicotyl, the later decrease of this level to be interpreted as inhibition of auxin transport...

The effect of hydroxamates on the growth of the algaScenedesmus obliquus

Ludmila Lisá, S. Prát

Biologia plantarum 8:476-478, 1966 | DOI: 10.1007/BF02930943  

The effect of hydrocortison on the transmission of potato witches' broom

M. Ulrychová, J. Limberk

Biologia plantarum 8:479, 1966 | DOI: 10.1007/BF02930944