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Molecular characterization of Brassica napus stress related transcription factors, BnMYB44 and BnVIP1, selected based on comparative analysis of Arabidopsis thaliana and Eutrema salsugineum transcriptomes

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Abstract

Many studies have been performed to identify regulatory circuit underlying plant stress tolerance. However, the reliability of some findings has been criticized because of exclusive use of stress sensitive plant species such as Arabidopsis thaliana. Sensitive plant species often harbor narrow defensive mechanisms and have relatively low capacity for adaptive responses. Therefore, it is useful to employ tolerant model plants, such as Eutrema salsugineum, to provide comprehensive insights into various mechanisms involved in response to abiotic stresses. In this study, comparative transcriptome and regulatory network analysis of stress-sensitive (A. thaliana) and -tolerant (E. salsugineum) model plants uncovered regulatory hierarchies underlying response to abiotic stresses and suggested the transcription factor genes, MYB44 and VIP1 as the candidate hub genes to perform molecular analyses on their Brassica napus homologs, BnMYB44 and BnVIP1. The full-length coding sequence of BnMYB44 and BnVIP1 with 891 and 969 bp long were cloned and sequenced. They shared high similarity with their counterparts in other plants at nucleotide and amino acid levels. The expression patterns of BnMYB44 and BnVIP1 genes of the two B. napus cultivars under drought and salt stress conditions coupled with the data obtained from the physiological measurements as well as analysis of the BnMYB44 and BnVIP1 promoters suggested that BnMYB44 and BnVIP1 genes may contribute to responses to drought and salt stresses in B. napus.

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Acknowledgements

This research was supported by Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University.

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Authors and Affiliations

  1. Department of Crop Production and Plant Breeding, School of Agriculture, Shiraz University, Shiraz, Iran

    Roohollah Shamloo-Dashtpagerdi, Hooman Razi & Esmaeil Ebrahimie

  2. Department of Agriculture and Natural Resources, Higher Education Center of Eghlid, Eghlid, Iran

    Roohollah Shamloo-Dashtpagerdi

  3. Institute of Biotechnology, Shiraz University, Shiraz, Iran

    Esmaeil Ebrahimie & Ali Niazi

  4. Adelaide Medical School, The University of Adelaide, Adelaide, Australia

    Esmaeil Ebrahimie

  5. Division of Information Technology, Engineering and the Environment, School of Information Technology and Mathematical Sciences, University of South Australia, Adelaide, Australia

    Esmaeil Ebrahimie

  6. School of Biological Sciences, Faculty of Science and Engineering, Flinders University, Adelaide, Australia

    Esmaeil Ebrahimie

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  1. Roohollah Shamloo-Dashtpagerdi
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Correspondence to Hooman Razi or Esmaeil Ebrahimie.

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Shamloo-Dashtpagerdi, R., Razi, H., Ebrahimie, E. et al. Molecular characterization of Brassica napus stress related transcription factors, BnMYB44 and BnVIP1, selected based on comparative analysis of Arabidopsis thaliana and Eutrema salsugineum transcriptomes. Mol Biol Rep 45, 1111–1124 (2018). https://doi.org/10.1007/s11033-018-4262-0

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