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Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles

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Nanoparticles in Biology and Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2118))

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Abstract

Thrombin, a major protein involved in the clotting cascade by the conversion of inactive fibrinogen to fibrin, plays a crucial role in the development of thrombosis. Antithrombin nanoparticles enable site-specific anticoagulation without increasing bleeding risk. Here we outline the process of making and the characterization of bivalirudin and d-phenylalanyl-l-prolyl-l-arginyl-chloromethyl ketone (PPACK) nanoparticles. Additionally, the characterization of these nanoparticles, including particle size, zeta potential, and quantification of PPACK/bivalirudin loading, is also described.

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Acknowledgments

The manuscript was edited by Enrico Ferrari and Mikhail Soloviev.

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Authors and Affiliations

  1. The USF Health Heart Institute, University of South Florida, Tampa, FL, USA

    Alexander J. Wilson, Ian Vargas, Ryan Grabau, Hua Pan & Samuel A. Wickline

  2. College of Pharmacy, University of South Florida, Tampa, FL, USA

    Qingyu Zhou

  3. Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA

    Rohun Palekar

Authors
  1. Alexander J. Wilson
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  2. Qingyu Zhou
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  3. Ian Vargas
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  4. Rohun Palekar
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  5. Ryan Grabau
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  6. Hua Pan
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  7. Samuel A. Wickline
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Corresponding author

Correspondence to Samuel A. Wickline .

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Editors and Affiliations

  1. College of Science, School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln, Lincolnshire, UK

    Enrico Ferrari

  2. Centre for Biomedical Sciences, Department of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK

    Mikhail Soloviev

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Wilson, A.J. et al. (2020). Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles. In: Ferrari, E., Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 2118. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0319-2_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0319-2_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0318-5

  • Online ISBN: 978-1-0716-0319-2

  • eBook Packages: Springer Protocols

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