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Superoxide dismutase derivative prevents oxidative damage in liver and kidney of rats induced by exhausting exercise

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Abstract

To prevent oxidative tissue damage induced by strenuous exercise in the liver and kidney superoxide dismutase derivative (SM-SOD), which circulated bound to albumin with a half-life of 6 h, was injected intraperitoneally into rats. Exhausting treadmill running caused a significant increase in the activities of xanthine oxidase (XO), and glutathione peroxidase (GPX) in addition to concentrations of thiobarbituric acid-reactive substances (TBARS) in hepatic tissue immediately after running. There was a definite increase in the immunoreactive content of mitochondrial superoxide dismutase (Mn-SOD) 1 day after the running. Meanwhile, the TBARS concentration in the kidney was markedly elevated 3 days after running. The activities of GPX, and catalase in the kidney increased significantly immediately and on days 1 and 3 following the test. The immunoreactive content of Mn-SOD also increased 1 day after running. The exercise induced no significant changes in immunoreactive Cu, Zn-SOD content in either tissue. The administration of SM-SOD provided effective protection against lipid peroxidation, and significantly attenuated the alterations in XO and all the anti-oxidant enzymes, measured. In summary, the present data would suggest that exhausting exercise may induce XO-derived oxidative damage in the liver, while the increase in lipid peroxidation in the kidney might be the result of washout-dependent accumulation of peroxidised metabolites. We found that the administration of SM-SOD provided excellent protection against exercise-induced oxidative stress in both liver and kidney.

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Authors and Affiliations

  1. Laboratory of Exercise Physiology, Institute of Health and Sport Sciences, University of Tsukuba, 1-1-1, Tennodai, 305, Tsukuba, Japan

    Zsolt Radák & Katsumi Asano

  2. Department of Biochemistry I, Osaka City University Medical School, 545, Osaka, Japan

    Masayasu Inoue

  3. Department of Hygiene, National Defence Medical College, 359, Tokorozawa, Japan

    Takako Kizaki, Shuji Oh-Ishi & Hideki Ohno

  4. Department of Biochemistry, Osaka University Medical School, 565, Suita, Japan

    Keiichiro Suzuki & Naoyuki Taniguchi

Authors
  1. Zsolt Radák
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  2. Katsumi Asano
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  3. Masayasu Inoue
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  4. Takako Kizaki
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  5. Shuji Oh-Ishi
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  6. Keiichiro Suzuki
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  7. Naoyuki Taniguchi
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  8. Hideki Ohno
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Radák, Z., Asano, K., Inoue, M. et al. Superoxide dismutase derivative prevents oxidative damage in liver and kidney of rats induced by exhausting exercise. Europ. J. Appl. Physiol. 72, 189–194 (1996). https://doi.org/10.1007/BF00838637

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  • DOI: https://doi.org/10.1007/BF00838637

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