Abstract
The nucleotide sequences of the internal transcribed spacers (ITS1 and ITS2) of the nuclear ribosomal DNA were analyzed in species belonging to gymnosperms and pteridophytes. The lengths of the ITSs of sixteen species of gymnosperms and seven species of pteridophytes were estimated. The gymnosperms have ITS1 regions larger than those observed in the pteridophytes and angiosperms (ca. 610–3100 bp versus 159–360 bp). On the other hand, the ITS2 regions appear to be of a conserved length (182–370 bp). We have determined the complete nucleotide sequences of ITS regions from four gymnosperm species and five pteridophyte species by cloning the PCR products. Sequence analysis showed the presence of three short tandem arranged subrepeats of about 70 bp in the 1112 bp ITS1 ofEphedra fragilis. Pyrimidine rich (about 90%) DNA segments of 40–50 bp were observed in the ITS1 ofGinkgo biloba. A highly conserved 16 bp long sequence known to be present in the ITS1 of the angiosperm species has been also found in the ITS1 ofCycas revoluta, Taxus baccata andEphedra fragilis.
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References
Baldwin, B. G., 1992: Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from theCompositae. — Molec. Phylogenet. Evol.1: 3–16.
—, 1995: The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. — Ann. Missouri Bot. Gard.82: 247–277.
Bobola, M. S., Smith, D. E., Klein, A. S., 1992: Five major nuclear ribosomal repeats represent a large and variable fraction of the genomic DNA ofPicea rubens andP. mariana. — Molec. Biol. Evol.9: 125–137.
Karvonen, P., Savolainen, O., 1993: Variation and inheritance of ribosomal DNA inPinus sylvestris L. (Scots pine). — Heredity71: 614–622.
—, 1994: Length variation in the internal transcribed spacers of ribosomal DNA inPicea abies and related species. — Theor. Appl. Genet.89: 969–974.
Kim, K. J., Jansen, R. K., 1994: Comparisons of phylogenetic hypotheses among different data sets in dwarf dandelions (Krigia, Asteraceae): additional information from internal transcribed spacer sequences of nuclear ribosomal DNA. — Pl. Syst. Evol.190: 157–185.
Liston, A., Alvarez-Buylla, E., Robinson, W., Oliphant, J. M., 1995: Length variation in the nuclear ribosomal DNA ITS ofConiferales, Ginkgoales, andGnetales. — Amer. J. Bot.82 (Abstr.): 146.
Liu, J. S., Schardl, C. L., 1994: A conserved sequence in internal transcribed spacer 1 of plant nuclear rRNA genes. — Pl. Molec. Biol.26: 775–778.
Maggini, F., Barsanti, P., Marazia, T., 1978: Individual variation of the nucleolus organizer regions inAllium cepa andA. sativum. — Chromosoma66: 173–183.
—, 1995: Ribosomal RNA genes in the genusPinus. I. — Caryologia48: 17–25.
Marrocco, R., Gelati, M. T., Maggini, F., 1996: Nucleotide sequence of the internal transcribed spacers and 5.8 S region of ribosomal DNA inPinus pinea L. — DNA Sequence6: 175–177.
Rogers, S. O., Bendich, A. J., 1987: Ribosomal RNA genes in plants: variability in copy number and in intergenic spacer. — Pl. Molec. Biol.9: 509–520.
Sanger, F., Nicklen, S., Coulson, A. R., 1977: DNA sequencing with chain-terminating inhibitors. — Proc. Natl. Acad. Sci. USA74: 5463–5467.
Smith, D. E., Klein, A. S., 1996: Erratum. — Molec. Phylogenet. Evol.5: 286–287.
Suh, Y. L., Thien, L. B., Reeve, H. E., Zimmer, E. Z., 1993: Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of ribosomal DNA inWinteraceae. — Amer. J. Bot.80: 1042–1055.
Torres, R. A., Ganal, M., Hemleben, V., 1990: GC balance in the internal transcribed spacers ITS1 and ITS2 of nuclear ribosomal RNA genes. — J. Molec. Evol.30: 170–181.
Troitsky, A. V., Melekhovets, Yu, F., Rakhimova, G. M., Bobrova, V. K., Valiejo-Roman, K. M., Antonov, A. S., 1991: Angiosperm origin and early stages of seed plant evolution deduced from rRNA sequence comparisons. — J. Molec. Evol.32: 253–261.
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Dedicated to Prof.Emilio Battaglia.
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Maggini, F., Marrocco, R., Gelati, M.T. et al. Lengths and nucleotide sequences of the internal spacers of nuclear ribosomal DNA in gymnosperms and pteridophytes. Pl Syst Evol 213, 199–205 (1998). https://doi.org/10.1007/BF00985200
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DOI: https://doi.org/10.1007/BF00985200