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The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr.

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Abstract

Plant cell and tissue cultures are considered as a source of valuable secondary metabolites but usually produce insufficient level of the compounds, which is the limiting factor for their application in biotechnology. We obtained 18 callus cell cultures from different organs of wild grape Vitis amurensis Rupr. collected at different seasons and analyzed stilbene accumulation in combination with calli growth parameters. This analysis showed that temporal and tissue origin of the calli affected the rate of stilbene biosynthesis. Stem-derived calli accumulated higher stilbene levels and exhibited a higher expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes than calli derived from the leaves and petioles. The highest content of stilbenes was detected in the calli initiated from grapevine stems collected in the autumn. In general, all “autumn” cell cultures contained more than 2 mg g− 1 dry wt (up to 11 mg g− 1 dry wt) and exhibited high PAL and STS genes expression in comparison with the calli initiated in the summer. The content of stilbenes in the “autumn” cell cultures were comparable to the highest stilbene contents detected in other plant sources described in the literature. Thus, selecting the most optimal explant source for cell culture establishment could be an effective approach towards developing plant cell cultures producing high stilbene levels.

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Fig. 1
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Abbreviations

HPLC:

High-performance liquid chromatography

STS:

Stilbene synthase

Wt:

Weight

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Acknowledgements

This work was supported by a Grant from the Russian Science Foundation (17-74-10082).

Author information

Authors and Affiliations

  1. Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia, 690022

    A. P. Tyunin, A. R. Suprun, N. N. Nityagovsky, A. Y. Manyakhin, A. S. Dubrovina & K. V. Kiselev

  2. Department of Biodiversity, The School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia, 690090

    A. R. Suprun, N. N. Nityagovsky, Y. A. Karetin & K. V. Kiselev

  3. Laboratory of Embryology, National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia, 690059

    Y. A. Karetin

Authors
  1. A. P. Tyunin
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  2. A. R. Suprun
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  3. N. N. Nityagovsky
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  4. A. Y. Manyakhin
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  5. Y. A. Karetin
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  6. A. S. Dubrovina
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  7. K. V. Kiselev
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Contributions

ASD and KKV were involved in research design, data analysis, and paper preparation. APT, ARS, and NNN were involved in plant cell cultures management and qRT-PCR. MAY performed HPLC. YAK: analyzed the data.

Corresponding author

Correspondence to K. V. Kiselev.

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We declare that we have no conflict of interest.

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Communicated by Klaus Eimert.

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Tyunin, A.P., Suprun, A.R., Nityagovsky, N.N. et al. The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr.. Plant Cell Tiss Organ Cult 136, 189–196 (2019). https://doi.org/10.1007/s11240-018-1490-x

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  • DOI: https://doi.org/10.1007/s11240-018-1490-x

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