Abstract
Ultraviolet radiations (UV) are the primary causative agent for skin aging (photoaging) and cancer, especially UV-A. The mode of action and the molecular mechanism behind the damages caused by UV-A is not well studied, in vivo. The current study was employed to investigate the impact of UV-A exposure using the model organism, Caenorhabditis elegans. Analysis of lifespan, healthspan, and other cognitive behaviors were done which was supported by the molecular mechanism. UV-A exposure on collagen damages the synthesis and functioning which has been monitored kinetically using engineered strain, col-19:: GFP. The study results suggested that UV-A accelerated the aging process in an insulin-like signaling pathway dependent manner. Mutant (daf-2)-based analysis concrete the observations of the current study. The UV-A exposure affected the usual behavior of the worms like pharyngeal movements and brood size. Quantitative PCR profile of the candidate genes during UV-A exposure suggested that continuous exposure has damaged the neural network of the worms, but the mitochondrial signaling and dietary restriction pathway remain unaffected. Western blot analysis of HSF-1 evidenced the alteration in protein homeostasis in UV-A exposed worms. Outcome of the current study supports our view that C. elegans can be used as a model to study photoaging, and the mode of action of UV-A-mediated damages can be elucidated which will pave the way for drug developments against photoaging.
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Acknowledgments
We thank Caenorhabditis Genetics Center, which is funded by the National Institute of Health, National Center for Research Resources for providing C. elegans N2 WT, mutant strains and E. coli OP50. KB thankfully acknowledges the ITC India Ltd., Department of Biotechnology (DBT), University Grants Commission (UGC), Indian Council of Medical Research (ICMR) and Council of Scientific and Industrial Research (CSIR), Department of Science and Technology (DST), Government of India, New Delhi, India, for the financial assistances. PMI wishes to thank ITC and CSIR, India, for the financial assistance (AU-ITC JRF & CSIR-SRF). The authors also gratefully acknowledge the use of the Bioinformatics Infrastructure Facility, Alagappa University funded by the Department of Biotechnology, Ministry of Science and Technology, Government of India (No. BT/BI/25/015/2012), the Instrumentation Facility provided by DST, Government of India through PURSE [Grant No.SR/S9Z-23/2010/42(G)] & FIST (Grant No.SR-FST/LSI-087/2008) and UGC, New Delhi through SAP-DRS1 [Grant No.F.3-28/2011(SAP-II)].
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Prasanth, M.I., Santoshram, G.S., Bhaskar, J.P. et al. Ultraviolet-A triggers photoaging in model nematode Caenorhabditis elegans in a DAF-16 dependent pathway. AGE 38, 27 (2016). https://doi.org/10.1007/s11357-016-9889-y
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DOI: https://doi.org/10.1007/s11357-016-9889-y