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Enhancement of Stilbene Biosynthesis in Grape-Cell Cultures by Natural Products Based on Endophytes of the Wild Grape Species Vitis amurensis RUPR.

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Abstract

A new approach based on endophyte products is proposed to enhance the biosynthesis of stilbenes (compounds with established health benefits) and to alter their qualitative profile in grape-cell cultures. For this, 13 products are developed based on bacterial and fungal endophytes from the wild-grown grape species Vitis amurensis Rupr. With the use of bacterial and fungal endophyte based-products, the stilbene content increased by a factor of 1.3–1.5 and 2.0–3.5, respectively. The highest increase in the stilbene content (up to 3.07 mg/g on a dry cell biomass bases) is observed in grape cells cultured for 3 days in the presence of an endophyte product based on a Trichoderma sp. (10 mg). It is established that the increase in the stilbene content observed in V. amurensis grape cells cultured in the presence of endophyte products is due to a significant upregulation of genes responsible for stilbene biosynthesis, i.e., phenylalanine ammonia lyase (PAL) and stilbene synthase (STS). Products based on native endophytes from V. amurensis are attractive and environmentally friendly stimulators for stilbene biosynthesis in grape-cell cultures.

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Funding

The work was supported by the Russian Science Foundation (project no. 20-74-00002).

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Authors and Affiliations

  1. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    O. A. Aleynova, N. N. Nityagovsky, A. R. Suprun & K. V. Kiselev

Authors
  1. O. A. Aleynova
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  2. N. N. Nityagovsky
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  3. A. R. Suprun
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  4. K. V. Kiselev
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Correspondence to O. A. Aleynova.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Kukharuk

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Aleynova, O.A., Nityagovsky, N.N., Suprun, A.R. et al. Enhancement of Stilbene Biosynthesis in Grape-Cell Cultures by Natural Products Based on Endophytes of the Wild Grape Species Vitis amurensis RUPR.. Appl Biochem Microbiol 58, 45–56 (2022). https://doi.org/10.1134/S0003683822010021

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