Inhibition of Glioblastoma Growth by the Thiadiazolidinone Compound TDZD-8
Figure 4
Effect of TDZD-8 treatment on apoptosis.
(A) Different glioblastoma cell lines were grown on glass coverslips, treated with TDZD-8 for different times and active caspase-3 was analyzed by immunofluorescence using a specific anti-active caspase-3 antibody. (B) GL261 glioblastoma cells were grown for 24 h on glass coverslips, treated with TDZD-8 or TDZD-8 plus zVAD-fmk and active caspase-3 was analyzed by immunofluorescence using a specific anti-active caspase-3 antibody. Scale bar, 25 µm. (C) GL261 glioblastoma cells were grown for 24 h on glass coverslips, treated with TDZD-8 or TDZD-8 plus zVAD-fmk and TUNEL analysis was performed. Representative immunofluorescence images are shown. Scale bar, 25 µm. Quantification of TUNEL-positive cells 24 h after TDZD-8 treatment is shown in the right panel. B, basal non-treated cells. Values are the means ± SD of at least three different experiments.