Highly multiplexed single-cell quantitative PCR
Fig 4
(A) Heatmap of expression values of 20 miRNAs in each of 95 K562 cells (green) and 81 BaF3 cells (purple). Unsupervised hierarchical clustering correctly groups the cell types based on their miRNA expression signatures. (B) Scatterplots of co-expressed miRNAs. miRNAs 17-5p, 20a-5p and 92a-3p are all strongly co-expressed as they are members of the miR-17~92 polycistronic cluster. The co-expression of miRNA 24-3p with these three miRNAs demonstrates how single-cell resolution identifies their positive regulation where bulk measurements would predict their negative regulation. Spearman correlation coefficients and their co-expression significance are denoted for each pair; * p < 0.05, ** p < 0.01, *** p < 0.001, ns not significant.