Fig. 4.
In vivo and in vitro comparison of glutamate-evoked Ca2+ transients. (A Upper) A high-magnification image of layer 2/3 neurons in vivo in the cortex of a P13 mouse. (Lower) Ca2+ transients in three individual neurons (marked in Upper) evoked by five consecutive 500-ms iontophoretic glutamate applications. (B Upper) In vivo stained layer 2/3 neurons from the same mouse as in A visualized in vitro in a cortical slice. (Lower) Ca2+ transients in three individual neurons evoked by iontophoretic glutamate applications in control, in the presence of 10 μM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 50 μM dl-2-amino-5-phosphonovaleric acid (APV), and 20 min after wash-out of the drugs. (C) Line-scan Ca2+ measurements and simultaneous recordings of membrane potential changes evoked by a 500-ms iontophoretic glutamate application in a whole-cell patch-clamped layer 2/3 pyramidal neuron.