Figure 1.
miR-137 is enriched in neurons and is expressed in the dentate gyrus and the molecular layer of the hippocampus. (A) Identification of lineage specific miRNAs in A94-NSCs. Plotted are the ratios of RQ values determined by comparing A94-NSCs differentiated toward the neuron lineage to undifferentiated A94-NSCs over RQ values determined by comparing A94-NSCs differentiated toward the astrocyte lineage to undifferentiated A94-NSCs (Figure S1A and S1B). Ratios ≥8 were set to a value of 8. (B) miR-137 expression during neuronal differentiation of A94-NSCs for 0.5, 1, 2, 3, and 4 days (miR-137 expression calibrated to undifferentiated A94-NSCs, n=3, mean ± 95% CI). (C) Enrichment of miR-137 in E17 neurons as compared to mouse primary aNSCs (miR-137 expression calibrated to mouse primary aNSCs, n=3, mean ± SEM). (D–E) Hybridization with a miR-137-specific probe showed an enrichment of miR-137 within the DG and molecular layer of the hippocampus compared with miR-1, which is expressed at low levels in the CNS (h, hilus; g, dentate gyrus; m, molecular layer).