Figure 2. Effects of systemic macrophage depletion with clodrolip on the CCR2+ splenic cells.
A. Representative photos of spleen section showing CCR2+ cell repartition mainly in the red pulp (RP) and less in the white pulp (WP), 24 h after tibia fracture. Top photos are of low magnification (scale bar: 100 microm) and bottom photos are highly magnified images (scale bare: 50 microm) in the CT-lip and the clodrolip mice; B. Quantification relative percentage of CCR2+ cells in the spleen after clodrolip (n = 6, ***: P < 0.001 with unpaired t-test). CCR2 = chemokine (C-C motif) receptor 2; CT-lip = control liposome; DAPI = 4',6'-diamidino-2-phenylindole; IP = intraperitoneal. Bars are means ± 95% confidence interval.