Fig. 2.
Histological analysis of WAT of Tam-treated PPARγad–/– premutant mice. Histological sections from paraffin-embedded epididymal WAT from CT (aP2-Cre-ERT2(0/0)/PPARγL2/L2) mice at D4 (A) and D2 (F) and MT mice at D2 (G), D4 (H and I), D7 (B), D14 (J), and D42 (C) (Tam injection at D0), and D67 (D) and D116 (E), after a second Tam injection at D56. (A–I) Hematoxylin/eosin staining. (J) Trichrome staining. Ultrastructure of adipose tissue of CT (aP2-Cre-ERT2(0/0)/PPARγL2/L2) at D4 (K) and MT mice at D 4 (L) and D7 (M–O). Blue arrows point to clusters of infiltrating cells (B and D); black arrows point to adipocytes with abnormal profiles (G); white arrows point to small lipid droplets in adipocytes (G, M, and N) and to phagocytosed lipid droplets (O); green arrows point to neutrophils (H and I); red arrows point to lymphocytes (I and N); yellow arrows point to macrophages (I, N, and O); orange arrows point to fibroblast-like cells (J and N); and black and gray arrows point to disrupted adipocyte cell membrane and cell debris (L). Lp, lipid droplet; Nc, nucleus. (Scales are indicated in each image.)