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Sequence alignment of S proteins from HKU1, SARS-CoV and MERS-CoV using Clustal Omega52. Protein features described in the text are indicated: N-terminal domain (NTD), C-terminal domain (CTD) which contains the large variable loop, the S1/S2 and S2′ cleavage sites, fusion peptide (FP), heptad repeats 1 and 2 (HR1, HR2) and transmembrane helix (TM).
