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. 2019 Mar 26;24(6):1185. doi: 10.3390/molecules24061185

Table 2.

Transition ions, retention times (for each conjugation position), and relative contents of green tea catechins (GTCs) metabolites from the samples taken from the apical sides after loading catechins in the basolateral side. The relative content of the catechin metabolites were measured as free-form equivalents, relative to the internal standard (ethyl gallate). Each value was the average of three different samples. * and ** indicate significantly different at a 0.05 and 0.01 level between the metabolites from cis and trans catechins, respectively. Me indicates methyl; ND indicates not detected.

Compound Name Detected Mass (ESI-) MS/MS Fragments (Collision Energy) Retention Time (min) Relative Content (nmol) Significance
C-O-sulfate 369.0279 289, 231, 109 (20 V) 3.86 541.57 ± 65.43 *
EC-O-sulfate 369.0285 289, 245,137 (20 V) 6.69 202.50 ± 12.58
O-me-C 303.0879 248, 203, 159, 101, 73 (1 0V) 3.86, 9.25 41.07 ± 3.66 *
O-me-EC 303.089 248, 159, 73 (10 V) 7.40, 12.64 22.33 ± 1.85
O-me-C-O-sulfate ND ND ND ND
O-me-EC-O-sulfate ND ND ND ND
GC-O-sulfate 385.024 305, 261, 125 (20 V) 2.19 510.85 ± 61.72 **
EGC-O-sulfate 385.0246 305, 219, 154 (20 V) 2.54 31.77 ± 1.97
O-me-GC 319.0523 303, 194, 105 (10 V) 2.17, 7.47 18.54 ± 1.74 *
O-me-EGC 319.0523 253, 183, 93 (10V) 2.35, 8.10 30.10 ± 3.35
O-me-GC-O-sulfate 399.0395 385, 288, 124 (10 V) 2.20, 3.25 79.42 ± 6.50 *
O-me-EGC-O-sulfate 399.0404 319, 253, 183, 93 (10 V) 2.52, 4.23 23.14 ± 1.60
CG-O-sulfate 521.0416 441, 369, 289, 249, 169, 64 (20 V) 13.61 366.00 ± 44.22 **
ECG-O-sulfate 521.0406 441, 331, 289, 169, 125 (20 V) 16.28 7.25 ± 0.45
O-me-CG 455.0998 439, 382, 271, 169, 125, 58 (20 V) 14.69, 18.65, 19.08 50.78 ± 5.44 *
O-me-ECG 455.0989 352, 183, 109 (20 V) 14.13, 17.14 10.02 ± 0.80
O-me-CG-O-sulfate 535.0567 455, 397, 303, 198, 141, 89 (10 V) 15.69, 17.11 62.89 ± 6.10
O-me-ECG-O-sulfate ND ND ND ND
GCG-O-sulfate 537.0362 457, 395, 305, 216, 169, 125 (10 V) 7.48, 8.79, 9.76 216.70 ± 20.73 **
EGCG-O-sulfate 537.0376 457, 287, 169, 113 (10 V) 10.4 6.87 ± 0.55
O-me-GCG 471.0931 402, 287, 125 (10 V) 9.82, 14.61, 15.12 35.23 ± 3.67 *
O-me-EGCG 471.0954 305, 113 (10 V) 7.81, 12.19 11.51 ± 1.11
O-me-GCG-O-sulfate 551.0513 464, 399, 369, 324, 205, 157, 77 (10 V) 11.84, 13.50, 14.68 96.48 ± 7.80
O-me-EGCG-O-sulfate ND ND ND ND
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