Enhancement of TBP binding by activators and general transcription factors
- PMID: 10376604
- DOI: 10.1038/21232
Enhancement of TBP binding by activators and general transcription factors
Abstract
Eukaryotic transcriptional activators function, at least in part, by promoting assembly of the preinitiation complex, which comprises RNA polymerase II and its general transcription factors (GTFs). Activator-mediated stimulation of the assembly of the preinitiation complex has been studied in vitro but has been relatively refractory to in vivo analysis. Here we use a DNA-crosslinking/immunoprecipitation assay to study in living cells the first step in the assembly of the preinitiation complex, the interaction between the TATA-box-binding protein (TBP) and its binding site, the TATA box. Analysis of a variety of endogenous yeast genes, and of a series of activators of differing strength, reveals a general correlation between TBP binding and transcriptional activity. Using mutant yeast strains, we show that Mot1 prevents the binding of TBP to inactive promoters and that activator-mediated stimulation of TBP binding requires additional GTFs, including TFIIB and Srb4. Taken together, our results indicate that TBP binding in vivo is stringently controlled, and that the ability of activators to stimulate this step in the assembly of the preinitiation complex is a highly cooperative process involving multiple transcription factors.
Similar articles
-
Recruitment of TBP or TFIIB to a promoter proximal position leads to stimulation of RNA polymerase II transcription without activator proteins both in vivo and in vitro.Biochem Biophys Res Commun. 1999 Mar 5;256(1):45-51. doi: 10.1006/bbrc.1999.0280. Biochem Biophys Res Commun. 1999. PMID: 10066420
-
Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme.Nature. 1999 Jun 10;399(6736):609-13. doi: 10.1038/21239. Nature. 1999. PMID: 10376605
-
Eukaryotic activators function during multiple steps of preinitiation complex assembly.Nature. 1993 Dec 9;366(6455):531-6. doi: 10.1038/366531a0. Nature. 1993. PMID: 8255291
-
Mechanisms of transcriptional activation and repression can both involve TFIID.Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):517-26. doi: 10.1098/rstb.1996.0050. Philos Trans R Soc Lond B Biol Sci. 1996. PMID: 8735274 Review.
-
Retinoid-dependent transcription: the RAR/RXR-TBP-EIA/EIA-LA connection.Biochem Soc Symp. 1996;62:97-109. Biochem Soc Symp. 1996. PMID: 8971343 Review.
Cited by
-
The Mediator kinase module enhances polymerase activity to regulate transcriptional memory after heat stress in Arabidopsis.EMBO J. 2024 Feb;43(3):437-461. doi: 10.1038/s44318-023-00024-x. Epub 2024 Jan 16. EMBO J. 2024. PMID: 38228917 Free PMC article.
-
The N-Terminal Tail of Histone H3 Regulates Copper Homeostasis in Saccharomyces cerevisiae.Mol Cell Biol. 2021 Jan 25;41(2):e00210-20. doi: 10.1128/MCB.00210-20. Print 2021 Jan 25. Mol Cell Biol. 2021. PMID: 33257505 Free PMC article.
-
Role of the pre-initiation complex in Mediator recruitment and dynamics.Elife. 2018 Dec 12;7:e39633. doi: 10.7554/eLife.39633. Elife. 2018. PMID: 30540252 Free PMC article.
-
Transfer RNA genes experience exceptionally elevated mutation rates.Proc Natl Acad Sci U S A. 2018 Sep 4;115(36):8996-9001. doi: 10.1073/pnas.1801240115. Epub 2018 Aug 20. Proc Natl Acad Sci U S A. 2018. PMID: 30127029 Free PMC article.
-
TOR Facilitates the Targeting of the 19S Proteasome Subcomplex To Enhance Transcription Complex Assembly at the Promoters of the Ribosomal Protein Genes.Mol Cell Biol. 2018 Jun 28;38(14):e00469-17. doi: 10.1128/MCB.00469-17. Print 2018 Jul 15. Mol Cell Biol. 2018. PMID: 29712756 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
