Mechanisms of viral activators
- PMID: 10384288
- DOI: 10.1101/sqb.1998.63.243
Mechanisms of viral activators
Abstract
Adenovirus large E1A, Epstein-Barr virus Zebra, and herpes simplex virus VP16 were studied as models of animal cell transcriptional activators. Large E1A can activate transcription from a TATA box, a result that leads us to suggest that it interacts with a general transcription factor. Initial studies showed that large E1A binds directly to the TBP subunit of TFIID. However, analysis of multiple E1A and TBP mutants failed to support the significance of this in vitro interaction for the mechanism of activation. Recent studies to be reported elsewhere indicate that conserved region 3 of large E1A, which is required for its activation function, binds to one subunit of a multisubunit protein that stimulates in vitro transcription in response to large E1A and other activators. A method was developed for the rapid purification of TFIID approximately 25,000-fold to near homogeneity from a cell line engineered to express an epitope-tagged form of TBP. Purified TFIID contains 11 major TAFs ranging in mass from approximately 250 to 20 kD. Zta and VP16, but not large E1A, greatly stimulate the rate and extent of assembly of a TFIID-TFIIA complex on promoter DNA (DA complex). For VP16, this is a function of the carboxy-terminal activation subdomain. An excellent correlation was found between the ability of VP16C mutants to stimulate DA complex assembly and their ability to activate transcription in vivo. Consequently, for a subset of activation domains, DA complex assembly activity is an important component of the overall mechanism of activation.
Similar articles
-
A class of activation domains interacts directly with TFIIA and stimulates TFIIA-TFIID-promoter complex assembly.Mol Cell Biol. 1995 Nov;15(11):6465-73. doi: 10.1128/MCB.15.11.6465. Mol Cell Biol. 1995. PMID: 7565798 Free PMC article.
-
Requirement for transcription factor IIA (TFIIA)-TFIID recruitment by an activator depends on promoter structure and template competition.Mol Cell Biol. 1997 Nov;17(11):6624-32. doi: 10.1128/MCB.17.11.6624. Mol Cell Biol. 1997. PMID: 9343426 Free PMC article.
-
Functional interaction of adenovirus E1A with holo-TFIID.Genes Dev. 1993 Sep;7(9):1810-23. doi: 10.1101/gad.7.9.1810. Genes Dev. 1993. PMID: 8370528
-
Mechanisms of transcriptional activation and repression can both involve TFIID.Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):517-26. doi: 10.1098/rstb.1996.0050. Philos Trans R Soc Lond B Biol Sci. 1996. PMID: 8735274 Review.
-
Retinoid-dependent transcription: the RAR/RXR-TBP-EIA/EIA-LA connection.Biochem Soc Symp. 1996;62:97-109. Biochem Soc Symp. 1996. PMID: 8971343 Review.
Cited by
-
Multiple domains in the 50 kDa form of E4F1 regulate promoter-specific repression and E1A trans-activation.Gene. 2020 Sep 5;754:144882. doi: 10.1016/j.gene.2020.144882. Epub 2020 Jun 11. Gene. 2020. PMID: 32535047 Free PMC article.
-
Divergent Evolution of E1A CR3 in Human Adenovirus Species D.Viruses. 2019 Feb 8;11(2):143. doi: 10.3390/v11020143. Viruses. 2019. PMID: 30744049 Free PMC article.
-
An Epigenetic Journey: Epstein-Barr Virus Transcribes Chromatinized and Subsequently Unchromatinized Templates during Its Lytic Cycle.J Virol. 2019 Apr 3;93(8):e02247-18. doi: 10.1128/JVI.02247-18. Print 2019 Apr 15. J Virol. 2019. PMID: 30700606 Free PMC article. Review.
-
Single-molecule FRET reveals a corkscrew RNA structure for the polymerase-bound influenza virus promoter.Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):E3335-42. doi: 10.1073/pnas.1406056111. Epub 2014 Jul 28. Proc Natl Acad Sci U S A. 2014. PMID: 25071209 Free PMC article.
-
Polycomb repressive complex 1 (PRC1) disassembles RNA polymerase II preinitiation complexes.J Biol Chem. 2012 Oct 19;287(43):35784-94. doi: 10.1074/jbc.M112.397430. Epub 2012 Aug 21. J Biol Chem. 2012. PMID: 22910904 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources