Evidence that transcription factor IIB is required for a post-assembly step in transcription initiation
- PMID: 10464320
- DOI: 10.1074/jbc.274.36.25807
Evidence that transcription factor IIB is required for a post-assembly step in transcription initiation
Abstract
Mutation of glutamate 62 to lysine in yeast transcription factor (TF) IIB (Sua7) causes a cold-sensitive phenotype. This mutant also leads to preferential transcription of downstream start sites on some promoters in vivo. To explore the molecular nature of these phenotypes, the TFIIB E62K mutant was characterized in vitro. The mutant interacts with TATA-binding protein normally. In three different assays, the mutant can also interact with RNA polymerase II and recruit it and the other basal transcription factors to a promoter. Despite the ability to assemble a transcription complex, the TFIIB E62K protein is severely defective in transcription in vitro. Therefore, the role of TFIIB must be more than simply bridging TATA-binding protein and polymerase at the promoter. We propose that the region around Glu-62 in yeast TFIIB plays a role in start site selection, perhaps mediating a conformational change in the polymerase or the DNA during the search for initiation sites. This step may be related to the yeast-specific spacing between TATA elements and start sites since mutations of the corresponding glutamate in mammalian TFIIB do not produce a similar effect.
Similar articles
-
Promoter-specific shifts in transcription initiation conferred by yeast TFIIB mutations are determined by the sequence in the immediate vicinity of the start sites.Mol Cell Biol. 2001 Jul;21(14):4427-40. doi: 10.1128/MCB.21.14.4427-4440.2001. Mol Cell Biol. 2001. PMID: 11416123 Free PMC article.
-
Characterization of sua7 mutations defines a domain of TFIIB involved in transcription start site selection in yeast.J Biol Chem. 1994 Dec 2;269(48):30569-73. J Biol Chem. 1994. PMID: 7982976
-
Promoter-specific activation defects by a novel yeast TBP mutant compromised for TFIIB interaction.Curr Biol. 2001 Nov 13;11(22):1794-8. doi: 10.1016/s0960-9822(01)00566-8. Curr Biol. 2001. PMID: 11719223
-
Archaeal transcription factors and their role in transcription initiation.FEMS Microbiol Rev. 1996 May;18(2-3):159-71. doi: 10.1111/j.1574-6976.1996.tb00234.x. FEMS Microbiol Rev. 1996. PMID: 8639326 Review.
-
X-ray crystallographic studies of eukaryotic transcription initiation factors.Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):483-9. doi: 10.1098/rstb.1996.0046. Philos Trans R Soc Lond B Biol Sci. 1996. PMID: 8735270 Review.
Cited by
-
Genome-wide analysis of TFIIB's role in termination of transcription.Res Sq [Preprint]. 2024 Jul 15:rs.3.rs-4619136. doi: 10.21203/rs.3.rs-4619136/v1. Res Sq. 2024. PMID: 39070618 Free PMC article. Preprint.
-
Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1.Biochem J. 2019 Apr 4;476(7):1053-1082. doi: 10.1042/BCJ20180701. Biochem J. 2019. PMID: 30885983 Free PMC article.
-
SILAC-MS Profiling of Reconstituted Human Chromatin Platforms for the Study of Transcription and RNA Regulation.J Proteome Res. 2018 Oct 5;17(10):3475-3484. doi: 10.1021/acs.jproteome.8b00395. Epub 2018 Sep 20. J Proteome Res. 2018. PMID: 30192551 Free PMC article.
-
The RNA polymerase II preinitiation complex. Through what pathway is the complex assembled?Transcription. 2014;5(1):e27050. doi: 10.4161/trns.27050. Transcription. 2014. PMID: 25764109 Free PMC article. Review.
-
Structural basis of transcription initiation by RNA polymerase II.Nat Rev Mol Cell Biol. 2015 Mar;16(3):129-43. doi: 10.1038/nrm3952. Epub 2015 Feb 18. Nat Rev Mol Cell Biol. 2015. PMID: 25693126 Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous