Stimulation of exocytosis without a calcium signal
- PMID: 10517797
- PMCID: PMC2269554
- DOI: 10.1111/j.1469-7793.1999.00023.x
Stimulation of exocytosis without a calcium signal
Abstract
More than 30 years ago, Douglas (Douglas & Rubin, 1961; Douglas, 1968) proposed that intracellular Ca2+ controls stimulus-secretion coupling in endocrine cells, and Katz & Miledi (1967; Katz, 1969) proposed that intracellular Ca2+ ions control the rapid release of neurotransmitters from synapses. These related hypotheses have been amply confirmed in subsequent years and for students of excitable cells, they dominate our teaching and research. Calcium controls regulated exocytosis. On the other hand, many studies of epithelial and blood cell biology emphasize Ca2+-independent regulation of secretion of mucin, exocytotic delivery of transporters and degranulation. The evidence seems good. Are these contrasting conclusions somehow mistaken, or are the dominant factors controlling exocytosis actually different in different cell types? In this essay, we try to reconcile these ideas and consider classes of questions to ask and hypotheses to test in seeking a more integrated understanding of excitation-secretion coupling. Our review is conceptual and narrowly selective of a few examples rather than referring to a broader range of useful studies in the extensive literature. The examples are taken from mammals and are documented principally by citing other reviews and two of our own studies. The evidence shows that protein phosphorylation by kinases potentiates Ca2+-dependent exocytosis and often suffices to induce exocytosis by itself. Apparently, protein phosphorylation is the physiological trigger in a significant number of examples of regulated exocytosis. We conclude that although sharing many common properties, secretory processes in different cells are specialized and distinct from each other.
Figures
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References
-
- Ammala C, Eliasson L, Bokvist K, Berggren PO, Honkanen RE, Sjøholm A, Rorsman P. Activation of protein kinases and inhibition of protein phosphatases play a central role in the regulation of exocytosis in mouse pancreatic beta cells. Proceedings of the National Academy of Sciences of the USA. 1994;91:4343–4347. - PMC - PubMed
-
- Avery J, Jahn R, Edwardson JM. Reconstitution of regulated exocytosis in cell-free systems: a critical appraisal. Annual Review of Physiology. 1999;61:777–807. - PubMed
-
- Brown D, Katsura T, Gustafson CE. Cellular mechanisms of aquaporin trafficking. American Journal of Physiology. 1998;275:F328–331. - PubMed
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