A mouse homolog of the Saccharomyces cerevisiae meiotic recombination DNA transesterase Spo11p
- PMID: 10534402
- DOI: 10.1006/geno.1999.5956
A mouse homolog of the Saccharomyces cerevisiae meiotic recombination DNA transesterase Spo11p
Abstract
The Saccharomyces cerevisiae Spo11 protein is thought to catalyze formation of the DNA double-strand breaks that initiate meiotic recombination. We have cloned cDNA and genomic DNA for a mouse gene encoding a protein with significant sequence similarity to conserved domains found in proteins of the Spo11p family. This putative mouse Spo11 gene maps to the distal region of chromosome 2 (homologous to human chromosome 20q13.2-q13.3) and comprises at least 12 exons, spanning approximately 15-18 kb. Strong expression of the Spo11 message is seen in juvenile and adult testis by RNA in situ hybridization, RT-PCR, and Northern blot, with much weaker expression in thymus and brain. In situ hybridization detects expression in oocytes of embryonic ovary, but not of adult ovary. RT-PCR and in situ hybridization analyses of a time course of juvenile testis development indicate that Spo11 expression begins in early meiotic Prophase I, prior to the pachytene stage, with increasing accumulation of mRNA through the pachytene stage. Taken together, these results strongly suggest that this gene encodes the functional homolog of yeast Spo11p, which in turn suggests that the mechanism of meiotic recombination initiation is conserved between yeast and mammals.
Copyright 1999 Academic Press.
Similar articles
-
Cloning, characterization, and localization of mouse and human SPO11.Genomics. 1999 Oct 15;61(2):156-69. doi: 10.1006/geno.1999.5955. Genomics. 1999. PMID: 10534401
-
Developmental stage- and germ cell-regulated expression of a calcium-binding protein mRNA in mouse Sertoli cells.Mol Reprod Dev. 1999 Nov;54(3):232-43. doi: 10.1002/(SICI)1098-2795(199911)54:3<232::AID-MRD4>3.0.CO;2-F. Mol Reprod Dev. 1999. PMID: 10497345
-
Gene expression profiles of Spo11-/- mouse testes with spermatocytes arrested in meiotic prophase I.Reproduction. 2006 Jul;132(1):67-77. doi: 10.1530/rep.1.00997. Reproduction. 2006. PMID: 16816334
-
[SPO11: an activity that promotes DNA breaks required for meiosis].Med Sci (Paris). 2004 Feb;20(2):213-8. doi: 10.1051/medsci/2004202213. Med Sci (Paris). 2004. PMID: 14997442 Review. French.
-
[Mechanisms and control of meiotic recombination in the yeast Saccharomyces cerevisiae].J Soc Biol. 1999;193(1):23-7. J Soc Biol. 1999. PMID: 10851552 Review. French.
Cited by
-
Recent advances in mechanisms ensuring the pairing, synapsis and segregation of XY chromosomes in mice and humans.Cell Mol Life Sci. 2024 Apr 23;81(1):194. doi: 10.1007/s00018-024-05216-0. Cell Mol Life Sci. 2024. PMID: 38653846 Free PMC article. Review.
-
The proper interplay between the expression of Spo11 splice isoforms and the structure of the pseudoautosomal region promotes XY chromosomes recombination.Cell Mol Life Sci. 2023 Sep 8;80(10):279. doi: 10.1007/s00018-023-04912-7. Cell Mol Life Sci. 2023. PMID: 37682311 Free PMC article.
-
The RNA-binding protein FUS/TLS interacts with SPO11 and PRDM9 and localize at meiotic recombination hotspots.Cell Mol Life Sci. 2023 Mar 26;80(4):107. doi: 10.1007/s00018-023-04744-5. Cell Mol Life Sci. 2023. PMID: 36967403 Free PMC article.
-
Testis-expressed gene 11 inhibits cisplatin-induced DNA damage and contributes to chemoresistance in testicular germ cell tumor.Sci Rep. 2022 Nov 1;12(1):18423. doi: 10.1038/s41598-022-21856-3. Sci Rep. 2022. PMID: 36319719 Free PMC article.
-
Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids.Genetics. 2021 Mar 3;217(1):1-14. doi: 10.1093/genetics/iyaa013. Genetics. 2021. PMID: 33683354 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases