Multiepitopic B- and T-cell responses induced in humans by a human immunodeficiency virus type 1 lipopeptide vaccine
- PMID: 10644339
- PMCID: PMC111644
- DOI: 10.1128/jvi.74.4.1694-1703.2000
Multiepitopic B- and T-cell responses induced in humans by a human immunodeficiency virus type 1 lipopeptide vaccine
Abstract
We have attempted to develop an anti-human immunodeficiency virus (HIV) lipopeptide vaccine with several HIV-specific long peptides modified by C-terminal addition of a single palmitoyl chain. A mixture of six lipopeptides derived from regulatory or structural HIV-1 proteins (Nef, Gag, and Env) was prepared. A phase I study was conducted to evaluate immunogenicity and tolerance in lipopeptide vaccination of HIV-1-seronegative volunteers given three injections of either 100, 250, or 500 microg of each lipopeptide, with or without immunoadjuvant (QS21). This report analyzes in detail B- and T-cell responses induced by vaccination. The lipopeptide vaccine elicited strong and multiepitopic B- and T-cell responses. Vaccinated subjects produced specific immunoglobulin G antibodies that recognized the Nef and Gag proteins. After the third injection, helper CD4(+)-T-cell responses as well as specific cytotoxic CD8(+) T cells were also obtained. These CD8(+) T cells were able to recognize naturally processed viral proteins. Finally, specific gamma interferon-secreting CD8(+) T cells were also detected ex vivo.
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References
-
- BenMohamed L, Gras-Masse H, Tartar A, Daubersies P, Brahimi K, Bossus M, Thomas A, Druilhe P. Lipopeptide immunization without adjuvant induces potent and long-lasting B, T helper, and cytotoxic T lymphocyte responses against a malaria liver stage antigen in mice and chimpanzees. Eur J Immunol. 1997;27:1242–1253. - PubMed
-
- Clements-Mann M L, Weinhold K, Matthews T J, Graham B S, Gorse G J, Keefer M C, McElrath M J, Hsieh R H, Mestecky J, Zolla-Pazner S, Mascola J, Schwartz D, Siliciano R, Corey L, Wright P F, Belshe R, Dolin R, Jackson S, Xu S, Fast P, Walker M C, Stablein D, Excler J L, Tartaglia J, Paoletti E A. v. e. Group. Immune responses to human immunodeficiency virus (HIV) type 1 induced by canarypox expressing HIV-1 MN gp120, HIV-1 SF2 recombinant gp120, or both vaccines in seronegative adults. J Infect Dis. 1998;177:1230–1246. - PubMed
-
- Connan F, Hlavac F, Hoebeke J, Guillet J G, Choppin J. A simple assay for detection of peptides promoting the assembly of HLA class I molecules. Eur J Immunol. 1994;24:777–780. - PubMed
-
- Connor R, Korber B, Graham B, Hahn B, Ho D D, Walker B, Neumann A, Vermund S, Mestecky J, Jackson S, Fenamore E, Cao Y, Gao F, Kalams S, Kunstman K, McDonald D, McWilliams N, Trkola A, Moore J, Woonsky S. Immunological and virological analyses of persons infected by human immunodeficiency virus type 1 while participating in trials of recombinant gp120 subunit vaccines. J Virol. 1998;72:1552–1576. - PMC - PubMed
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