doi: 10.1016/s1097-2765(00)80254-3.
The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs
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- PMID: 10882111
- DOI: 10.1016/s1097-2765(00)80254-3
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The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs
Mol Cell.
2000 Apr.
Free article
Abstract
PC2, the high-molecular weight constituent of the potent USA transcriptional coactivator fraction, was identified as a Mediator-like complex. Its composition resembles that of the TRAP/SMCC complex, but PC2 is distinguished by the prominent absence of the SRB10 and SRB11 kinase/cyclin pair, as well as several additional polypeptides. Furthermore, affinity-purified PC2, which lacks independent activity, acts in synergy with USA-derived coactivators PC3/topoisomerase I and PC4 to mediate the effects of a variety of activators (including VP16, the synthetic activator GAL4-AH, and the orphan nuclear receptor HNF4) and thus recapitulates partial USA coactivator function.
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