Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Sep 22;289(5487):2126-8.
doi: 10.1126/science.289.5487.2126.

Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae

S J Lin  1 , P A Defossez, L Guarente
Affiliations

Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae

S J Lin et al. Science. .

Abstract

Calorie restriction extends life-span in a wide variety of organisms. Although it has been suggested that calorie restriction may work by reducing the levels of reactive oxygen species produced during respiration, the mechanism by which this regimen slows aging is uncertain. Here, we mimicked calorie restriction in yeast by physiological or genetic means and showed a substantial extension in life-span. This extension was not observed in strains mutant for SIR2 (which encodes the silencing protein Sir2p) or NPT1 (a gene in a pathway in the synthesis of NAD, the oxidized form of nicotinamide adenine dinucleotide). These findings suggest that the increased longevity induced by calorie restriction requires the activation of Sir2p by NAD.

PubMed Disclaimer

Comment in

Publication types

MeSH terms

LinkOut - more resources

-