Changes in the community structure of ammonia-oxidizing bacteria during secondary succession of calcareous grasslands
- PMID: 11243267
- DOI: 10.1046/j.1462-2920.2000.00080.x
Changes in the community structure of ammonia-oxidizing bacteria during secondary succession of calcareous grasslands
Abstract
The community structure of beta-subclass Proteobacteria ammonia-oxidizing bacteria was determined in semi-natural chalk grassland soils at different stages of secondary succession. Both culture-mediated (most probable number; MPN) and direct nucleic acid-based approaches targeting genes encoding 16S rRNA and the AmoA subunit of ammonia monooxygenase were used. Similar shifts were detected in the composition of the ammonia oxidizer communities by both culture-dependent and independent approaches. A predominance of Nitrosospira sequence cluster 3 in early successional fields was replaced by Nitrosospira sequence cluster 4 in late successional fields. The rate of this shift differed between the two areas examined. This shift occurred in a background of relative stability in the dominant bacterial populations in the soil, as determined by domain-level polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Molecular analysis of enrichment cultures obtained using different ammonia concentrations revealed biases towards Nitrosospira sequence cluster 3 or Nitrosospira sequence cluster 4 under high- or low-ammonia conditions respectively. High-ammonia MPNs suggested a decease in ammonia oxidizer numbers with succession, but low-ammonia MPNs and competitive PCR targeting amoA failed to support such a trend. Ammonia turnover rate, not specific changes in plant diversity and species composition, is implicated as the major determinant of ammonia oxidizer community structure in successional chalk grassland soils.
Similar articles
-
Intensive management affects composition of betaproteobacterial ammonia oxidizers in turfgrass systems.Microb Ecol. 2008 Jul;56(1):178-90. doi: 10.1007/s00248-007-9335-x. Epub 2007 Nov 27. Microb Ecol. 2008. PMID: 18040734
-
Links between ammonia oxidizer species composition, functional diversity and nitrification kinetics in grassland soils.Environ Microbiol. 2005 May;7(5):676-84. doi: 10.1111/j.1462-2920.2005.00740.x. Environ Microbiol. 2005. PMID: 15819850
-
Patterns of community change among ammonia oxidizers in meadow soils upon long-term incubation at different temperatures.Appl Environ Microbiol. 2003 Oct;69(10):6152-64. doi: 10.1128/AEM.69.10.6152-6164.2003. Appl Environ Microbiol. 2003. PMID: 14532075 Free PMC article.
-
Grassland management regimens reduce small-scale heterogeneity and species diversity of beta-proteobacterial ammonia pxidizer populations.Appl Environ Microbiol. 2002 Jan;68(1):20-30. doi: 10.1128/AEM.68.1.20-30.2002. Appl Environ Microbiol. 2002. PMID: 11772604 Free PMC article.
-
Comparative diversity of ammonia oxidizer 16S rRNA gene sequences in native, tilled, and successional soils.Appl Environ Microbiol. 1999 Jul;65(7):2994-3000. doi: 10.1128/AEM.65.7.2994-3000.1999. Appl Environ Microbiol. 1999. PMID: 10388694 Free PMC article.
Cited by
-
Deep amoA amplicon sequencing reveals community partitioning within ammonia-oxidizing bacteria in the environmentally dynamic estuary of the River Elbe.Sci Rep. 2020 Oct 13;10(1):17165. doi: 10.1038/s41598-020-74163-0. Sci Rep. 2020. PMID: 33051504 Free PMC article.
-
Microbial Community Structure and Functional Potential in Cultivated and Native Tallgrass Prairie Soils of the Midwestern United States.Front Microbiol. 2018 Aug 15;9:1775. doi: 10.3389/fmicb.2018.01775. eCollection 2018. Front Microbiol. 2018. PMID: 30158906 Free PMC article.
-
Archaea Dominate the Ammonia-Oxidizing Community in Deep-Sea Sediments of the Eastern Indian Ocean-from the Equator to the Bay of Bengal.Front Microbiol. 2017 Mar 16;8:415. doi: 10.3389/fmicb.2017.00415. eCollection 2017. Front Microbiol. 2017. PMID: 28360898 Free PMC article.
-
Aboveground-belowground biodiversity linkages differ in early and late successional temperate forests.Sci Rep. 2015 Jul 17;5:12234. doi: 10.1038/srep12234. Sci Rep. 2015. PMID: 26184121 Free PMC article.
-
Shifts in Abundance and Diversity of Soil Ammonia-Oxidizing Bacteria and Archaea Associated with Land Restoration in a Semi-Arid Ecosystem.PLoS One. 2015 Jul 14;10(7):e0132879. doi: 10.1371/journal.pone.0132879. eCollection 2015. PLoS One. 2015. PMID: 26172994 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources