Analysis of the pobA and pobR genes controlling expression of p-hydroxybenzoate hydroxylase in Azotobacter chroococcum
- PMID: 11245981
- DOI: 10.1016/s0378-1119(00)00599-0
Analysis of the pobA and pobR genes controlling expression of p-hydroxybenzoate hydroxylase in Azotobacter chroococcum
Abstract
We report the cloning and analysis of a gene and its cognate regulatory element from a member of the Azotobacteriaceae which are involved in the breakdown of an aromatic compound. The genes from Azotobacter chroococcum encoding p-hydroxybenzoate hydroxylase (pobA) and its regulatory protein (pobR) were cloned from a genomic library and sequenced. Sequence analysis of pobA revealed homology with other bacterial p-hydroxybenzoate hydroxylase enzymes. Residues essential to the structure and function of the enzyme have been conserved. The pobR gene encodes a DNA binding regulatory protein with similarity to proteins from the AraC/XylS family of transcriptional activators. A fragment containing both pobA and pobR was cloned into pUC19 and p-hydroxybenzoate hydroxylase activity was induced in Escherichia coli by the addition of p-hydroxybenzoate. A frame-shift mutation introduced into the pobR gene prevented expression of p-hydroxybenzoate hydroxylase, indicating that PobR is the protein required for transcription of pobA. Interestingly, A. chroococcum PobR has no homology to the PobR protein that is the transcriptional activator of pobA in Acinetobacter strain ADP1, a protein that is homologous to the IclR family of transcriptional regulators. However, PobR from A. chroococcum is homologous to several other proteins, suggesting that these proteins will also function as transcriptional activators of pobA.
Similar articles
-
An update of the unceasingly growing and diverse AraC/XylS family of transcriptional activators.FEMS Microbiol Rev. 2021 Sep 8;45(5):fuab020. doi: 10.1093/femsre/fuab020. FEMS Microbiol Rev. 2021. PMID: 33837749 Review.
-
Members of the IclR family of bacterial transcriptional regulators function as activators and/or repressors.FEMS Microbiol Rev. 2006 Mar;30(2):157-86. doi: 10.1111/j.1574-6976.2005.00008.x. FEMS Microbiol Rev. 2006. PMID: 16472303 Review.
-
Combining localized PCR mutagenesis and natural transformation in direct genetic analysis of a transcriptional regulator gene, pobR.J Bacteriol. 1997 Jul;179(13):4270-6. doi: 10.1128/jb.179.13.4270-4276.1997. J Bacteriol. 1997. PMID: 9209043 Free PMC article.
-
Identification of the transcriptional activator pobR and characterization of its role in the expression of pobA, the structural gene for p-hydroxybenzoate hydroxylase in Acinetobacter calcoaceticus.J Bacteriol. 1993 Jul;175(14):4499-506. doi: 10.1128/jb.175.14.4499-4506.1993. J Bacteriol. 1993. PMID: 8331077 Free PMC article.
-
Regulation of p-hydroxybenzoate hydroxylase synthesis by PobR bound to an operator in Acinetobacter calcoaceticus.J Bacteriol. 1994 Jul;176(14):4277-84. doi: 10.1128/jb.176.14.4277-4284.1994. J Bacteriol. 1994. PMID: 8021213 Free PMC article.
Cited by
-
Unveiling the regulatory mechanisms of salicylate degradation gene cluster cehGHIR4 in Rhizobium sp. strain X9.Appl Environ Microbiol. 2023 Oct 31;89(10):e0080223. doi: 10.1128/aem.00802-23. Epub 2023 Oct 6. Appl Environ Microbiol. 2023. PMID: 37800922 Free PMC article.
-
Nanomolar Responsiveness of an Anaerobic Degradation Specialist to Alkylphenol Pollutants.J Bacteriol. 2020 Feb 11;202(5):e00595-19. doi: 10.1128/JB.00595-19. Print 2020 Feb 11. J Bacteriol. 2020. PMID: 31843798 Free PMC article.
-
Pyridine Nucleotide Coenzyme Specificity of p-Hydroxybenzoate Hydroxylase and Related Flavoprotein Monooxygenases.Front Microbiol. 2018 Dec 18;9:3050. doi: 10.3389/fmicb.2018.03050. eCollection 2018. Front Microbiol. 2018. PMID: 30631308 Free PMC article.
-
Simultaneous catabolism of plant-derived aromatic compounds results in enhanced growth for members of the Roseobacter lineage.Appl Environ Microbiol. 2013 Jun;79(12):3716-23. doi: 10.1128/AEM.00405-13. Epub 2013 Apr 5. Appl Environ Microbiol. 2013. PMID: 23563956 Free PMC article.
-
Transcriptomic analysis reveals a bifurcated terephthalate degradation pathway in Rhodococcus sp. strain RHA1.J Bacteriol. 2007 Mar;189(5):1641-7. doi: 10.1128/JB.01322-06. Epub 2006 Dec 1. J Bacteriol. 2007. PMID: 17142403 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources