MOR1 is essential for organizing cortical microtubules in plants
- PMID: 11385579
- DOI: 10.1038/35079128
MOR1 is essential for organizing cortical microtubules in plants
Abstract
Microtubules orchestrate cell division and morphogenesis, but how they disassemble and reappear at different subcellular locations is unknown. Microtubule organizing centres are thought to have an important role, but in higher plants microtubules assemble in ordered configurations even though microtubule organizing centres are inconspicuous or absent. Plant cells generate highly organized microtubule arrays that coordinate mitosis, cytokinesis and expansion. Inhibiting microtubule assembly prevents chromosome separation, blocks cell division and impairs growth polarity. Microtubules are essential for the formation of cell walls, through an array of plasma-membrane-associated cortical microtubules whose control mechanisms are unknown. Using a genetic strategy to identify microtubule organizing factors in Arabidopsis thaliana, we isolated temperature-sensitive mutant alleles of the MICROTUBULE ORGANIZATION 1 (MOR1) gene. Here we show that MOR1 is the plant version of an ancient family of microtubule-associated proteins. Point mutations that substitute single amino-acid residues in an amino-terminal HEAT repeat impart reversible temperature-dependent cortical microtubule disruption, showing that MOR1 is essential for cortical microtubule organization.
Similar articles
-
MICROTUBULE ORGANIZATION 1 regulates structure and function of microtubule arrays during mitosis and cytokinesis in the Arabidopsis root.Plant Physiol. 2006 Jan;140(1):102-14. doi: 10.1104/pp.105.069989. Epub 2005 Dec 23. Plant Physiol. 2006. PMID: 16377747 Free PMC article.
-
Spatial organization of plant cortical microtubules: close encounters of the 2D kind.Trends Cell Biol. 2009 Feb;19(2):62-71. doi: 10.1016/j.tcb.2008.11.004. Epub 2009 Jan 12. Trends Cell Biol. 2009. PMID: 19144522 Review.
-
Aberrant cell plate formation in the Arabidopsis thaliana microtubule organization 1 mutant.Plant Cell Physiol. 2005 Apr;46(4):671-5. doi: 10.1093/pcp/pci068. Epub 2005 Mar 7. Plant Cell Physiol. 2005. PMID: 15753108
-
The Arabidopsis CLASP gene encodes a microtubule-associated protein involved in cell expansion and division.Plant Cell. 2007 Sep;19(9):2763-75. doi: 10.1105/tpc.107.053777. Epub 2007 Sep 14. Plant Cell. 2007. PMID: 17873093 Free PMC article.
-
Microtubule cortical array organization and plant cell morphogenesis.Curr Opin Plant Biol. 2006 Dec;9(6):571-8. doi: 10.1016/j.pbi.2006.09.005. Epub 2006 Sep 28. Curr Opin Plant Biol. 2006. PMID: 17010658 Review.
Cited by
-
De novo TANGLED1 recruitment from the phragmoplast to aberrant cell plate fusion sites in maize.J Cell Sci. 2024 Jun 15;137(12):jcs262097. doi: 10.1242/jcs.262097. Epub 2024 Jun 19. J Cell Sci. 2024. PMID: 38832513 Free PMC article.
-
De Novo TANGLED1 Recruitment to Aberrant Cell Plate Fusion Sites in Maize.bioRxiv [Preprint]. 2024 Mar 8:2024.03.07.583939. doi: 10.1101/2024.03.07.583939. bioRxiv. 2024. Update in: J Cell Sci. 2024 Jun 15;137(12):jcs262097. doi: 10.1242/jcs.262097. PMID: 38496554 Free PMC article. Updated. Preprint.
-
Microtubule-associated phase separation of MIDD1 tunes cell wall spacing in xylem vessels in Arabidopsis thaliana.Nat Plants. 2024 Jan;10(1):100-117. doi: 10.1038/s41477-023-01593-9. Epub 2024 Jan 3. Nat Plants. 2024. PMID: 38172572
-
Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in Pinus yunnanensis.Int J Mol Sci. 2023 Sep 28;24(19):14658. doi: 10.3390/ijms241914658. Int J Mol Sci. 2023. PMID: 37834105 Free PMC article.
-
Anatomical observation and transcriptome analysis of branch-twisted mutations in Chinese jujube.BMC Genomics. 2023 Aug 29;24(1):500. doi: 10.1186/s12864-023-09572-2. BMC Genomics. 2023. PMID: 37644409 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases