Posttranslational mechanisms regulate the mammalian circadian clock
- PMID: 11779462
- DOI: 10.1016/s0092-8674(01)00610-9
Posttranslational mechanisms regulate the mammalian circadian clock
Abstract
We have examined posttranslational regulation of clock proteins in mouse liver in vivo. The mouse PERIOD proteins (mPER1 and mPER2), CLOCK, and BMAL1 undergo robust circadian changes in phosphorylation. These proteins, the cryptochromes (mCRY1 and mCRY2), and casein kinase I epsilon (CKIepsilon) form multimeric complexes that are bound to DNA during negative transcriptional feedback. CLOCK:BMAL1 heterodimers remain bound to DNA over the circadian cycle. The temporal increase in mPER abundance controls the negative feedback interactions. Analysis of clock proteins in mCRY-deficient mice shows that the mCRYs are necessary for stabilizing phosphorylated mPER2 and for the nuclear accumulation of mPER1, mPER2, and CKIepsilon. We also provide in vivo evidence that casein kinase I delta is a second clock relevant kinase.
Similar articles
-
Role of phosphorylation in the mammalian circadian clock.Cold Spring Harb Symp Quant Biol. 2007;72:167-76. doi: 10.1101/sqb.2007.72.036. Cold Spring Harb Symp Quant Biol. 2007. PMID: 18419274 Review.
-
[Synchronization and genetic redundancy in circadian clocks].Med Sci (Paris). 2008 Mar;24(3):270-6. doi: 10.1051/medsci/2008243270. Med Sci (Paris). 2008. PMID: 18334175 Review. French.
-
Direct association between mouse PERIOD and CKIepsilon is critical for a functioning circadian clock.Mol Cell Biol. 2004 Jan;24(2):584-94. doi: 10.1128/MCB.24.2.584-594.2004. Mol Cell Biol. 2004. PMID: 14701732 Free PMC article.
-
Daily variation of clock output gene activation in behaviorally arrhythmic mPer/mCry triple mutant mice.Chronobiol Int. 2003 Jul;20(4):683-95. doi: 10.1081/cbi-120022408. Chronobiol Int. 2003. PMID: 12916720
-
Control of intracellular dynamics of mammalian period proteins by casein kinase I epsilon (CKIepsilon) and CKIdelta in cultured cells.Mol Cell Biol. 2002 Mar;22(6):1693-703. doi: 10.1128/MCB.22.6.1693-1703.2002. Mol Cell Biol. 2002. PMID: 11865049 Free PMC article.
Cited by
-
Klebsiella pneumoniae alters zebrafish circadian rhythm via inflammatory pathways and is dependent on light cues.Heliyon. 2024 May 7;10(10):e30829. doi: 10.1016/j.heliyon.2024.e30829. eCollection 2024 May 30. Heliyon. 2024. PMID: 38770281 Free PMC article.
-
A conserved chronobiological complex times C. elegans development.bioRxiv [Preprint]. 2024 May 9:2024.05.09.593322. doi: 10.1101/2024.05.09.593322. bioRxiv. 2024. PMID: 38766223 Free PMC article. Preprint.
-
A review for the impacts of circadian disturbance on urological cancers.Sleep Biol Rhythms. 2023 Dec 5;22(2):163-180. doi: 10.1007/s41105-023-00500-1. eCollection 2024 Apr. Sleep Biol Rhythms. 2023. PMID: 38524168 Review.
-
Circadian Rhythms in Cardiovascular Metabolism.Circ Res. 2024 Mar 15;134(6):635-658. doi: 10.1161/CIRCRESAHA.123.323520. Epub 2024 Mar 14. Circ Res. 2024. PMID: 38484029 Review.
-
The Function, Regulation, and Mechanism of Protein Turnover in Circadian Systems in Neurospora and Other Species.Int J Mol Sci. 2024 Feb 22;25(5):2574. doi: 10.3390/ijms25052574. Int J Mol Sci. 2024. PMID: 38473819 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases