Secretion of IL-6, IL-11 and LIF by human cardiomyocytes in primary culture
- PMID: 12126642
- DOI: 10.1006/cyto.2002.1033
Secretion of IL-6, IL-11 and LIF by human cardiomyocytes in primary culture
Abstract
Interleukin (IL)-6-type cytokines are multifunctional proteins involved in cardiac hypertrophy and myocardial protection. Recent studies, performed on animal models, report the production of these cytokines by heart. The aim of this study was to analyse the capacity of myocytes and fibroblasts isolated from human atrium to secrete IL-6, leukaemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), IL-11, oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and the soluble receptor subunits sIL-6R and sgp130 during primary culture. We detected LIF, IL-11, sgp130 and a large amount of IL-6, but not OSM, CT-1, CNTF nor IL-6R in these culture supernatants. Both cardiomyocytes and fibroblasts are able to spontaneously produce IL-6. The increase of IL-6 production all along the culture period appears to be the consequence of fibroblast proliferation and gp130 stimulation. This is the first demonstration that human cardiac cells are able to secrete IL-6, but also LIF and IL-11 in vitro. These cytokines could be involved in an autocrine and/or a paracrine networks regulating myocardial cyto-protection, hypertrophy and fibrosis.
Similar articles
-
Interleukin-6 Family of Cytokines in Cancers.J Interferon Cytokine Res. 2024 Feb;44(2):45-59. doi: 10.1089/jir.2023.0103. Epub 2024 Jan 17. J Interferon Cytokine Res. 2024. PMID: 38232478 Review.
-
The cytokine receptor gp130: faithfully promiscuous.Sci STKE. 2003 Sep 23;2003(201):PE40. doi: 10.1126/stke.2003.201.pe40. Sci STKE. 2003. PMID: 14506288 Review.
-
Characterization of soluble gp130 released by melanoma cell lines: A polyvalent antagonist of cytokines from the interleukin 6 family.Clin Cancer Res. 1997 Aug;3(8):1443-51. Clin Cancer Res. 1997. PMID: 9815830
-
Differential temporal expression of mRNAs for ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), interleukin-6 (IL-6), and their receptors (CNTFR alpha, LIFR beta, IL-6R alpha and gp130) in injured peripheral nerves.Brain Res. 1998 May 18;793(1-2):321-7. doi: 10.1016/s0006-8993(98)00242-x. Brain Res. 1998. PMID: 9630704
-
Functional requirement of gp130-mediated signaling for growth and survival of mouse primordial germ cells in vitro and derivation of embryonic germ (EG) cells.Development. 1996 Apr;122(4):1235-42. doi: 10.1242/dev.122.4.1235. Development. 1996. PMID: 8620850
Cited by
-
Human myofibroblasts increase the arrhythmogenic potential of human induced pluripotent stem cell-derived cardiomyocytes.Cell Mol Life Sci. 2023 Sep 5;80(9):276. doi: 10.1007/s00018-023-04924-3. Cell Mol Life Sci. 2023. PMID: 37668685 Free PMC article.
-
The Microenvironment of the Pathogenesis of Cardiac Hypertrophy.Cells. 2023 Jul 4;12(13):1780. doi: 10.3390/cells12131780. Cells. 2023. PMID: 37443814 Free PMC article. Review.
-
Electrocardiographic abnormalities in patients with COVID-19 pneumonia and raised interleukin-6.J Family Med Prim Care. 2022 Oct;11(10):5902-5908. doi: 10.4103/jfmpc.jfmpc_135_22. Epub 2022 Oct 31. J Family Med Prim Care. 2022. PMID: 36618155 Free PMC article.
-
Inflammageing and Cardiovascular System: Focus on Cardiokines and Cardiac-Specific Biomarkers.Int J Mol Sci. 2023 Jan 3;24(1):844. doi: 10.3390/ijms24010844. Int J Mol Sci. 2023. PMID: 36614282 Free PMC article. Review.
-
β-Adrenergic Receptor Stimulation Maintains NCX-CaMKII Axis and Prevents Overactivation of IL6R-Signaling in Cardiomyocytes upon Increased Workload.Biomedicines. 2022 Jul 8;10(7):1648. doi: 10.3390/biomedicines10071648. Biomedicines. 2022. PMID: 35884952 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials