Modulation of bft expression by the Bacteroides fragilis pathogenicity island and its flanking region
- PMID: 12180925
- DOI: 10.1046/j.1365-2958.2002.03077.x
Modulation of bft expression by the Bacteroides fragilis pathogenicity island and its flanking region
Abstract
To establish a recombinant system for high-level expression of biologically active Bacteroides fragilis toxin (BFT), we studied the expression of bft in non-toxigenic B. fragilis (NTBF) strains. The bft gene and the B. fragilis pathogenicity island (BfPAI) were cloned into NTBF strains with two distinct genetic patterns: (i) pattern II, strains lacking the BfPAI and its flanking region; and (ii) pattern III, strains lacking the BfPAI but containing its flanking region. Analysis of BFT activity of these recombinant strains on HT29/C1 cells showed that both the BfPAI and its flanking regions are important to optimal BFT activity. Reverse transcription polymerase chain reaction (RT-PCR) analysis indicated that the BfPAI and its flanking regions modulate bft expression. Further experiments demonstrated that the approximately 700 bp region upstream of bft is the BfPAI region critical for optimal bft expression. We conclude that both the region flanking the BfPAI and approximately 700 bp region upstream of bft are crucial to maximal BFT production by ETBF strains.
Similar articles
-
Enterotoxigenic Bacteroides fragilis: a rogue among symbiotes.Clin Microbiol Rev. 2009 Apr;22(2):349-69, Table of Contents. doi: 10.1128/CMR.00053-08. Clin Microbiol Rev. 2009. PMID: 19366918 Free PMC article. Review.
-
Characterization of the Bacteroides fragilis pathogenicity island in human blood culture isolates.Anaerobe. 2006 Feb;12(1):17-22. doi: 10.1016/j.anaerobe.2005.06.005. Epub 2005 Aug 15. Anaerobe. 2006. PMID: 16701608
-
Non-toxigenic pattern II and III Bacteroides fragilis strains: coexistence in the same host.Res Microbiol. 2004 Sep;155(7):522-4. doi: 10.1016/j.resmic.2004.04.008. Res Microbiol. 2004. PMID: 15313251
-
The toxins of Bacteroides fragilis.Toxicon. 2001 Nov;39(11):1737-46. doi: 10.1016/s0041-0101(01)00160-x. Toxicon. 2001. PMID: 11595636 Review.
-
Molecular evolution of the pathogenicity island of enterotoxigenic Bacteroides fragilis strains.J Bacteriol. 1999 Nov;181(21):6623-33. doi: 10.1128/JB.181.21.6623-6633.1999. J Bacteriol. 1999. PMID: 10542162 Free PMC article.
Cited by
-
Bacteroides fragilis aggravates high-fat diet-induced non-alcoholic fatty liver disease by regulating lipid metabolism and remodeling gut microbiota.Microbiol Spectr. 2024 Apr 2;12(4):e0339323. doi: 10.1128/spectrum.03393-23. Epub 2024 Feb 27. Microbiol Spectr. 2024. PMID: 38411057 Free PMC article.
-
A systemic review of the role of enterotoxic Bacteroides fragilis in colorectal cancer.Neoplasia. 2022 Jul;29:100797. doi: 10.1016/j.neo.2022.100797. Epub 2022 Apr 20. Neoplasia. 2022. PMID: 35461079 Free PMC article.
-
Glucosylceramide production maintains colon integrity in response to Bacteroides fragilis toxin-induced colon epithelial cell signaling.FASEB J. 2020 Dec;34(12):15922-15945. doi: 10.1096/fj.202001669R. Epub 2020 Oct 13. FASEB J. 2020. PMID: 33047400 Free PMC article.
-
A Two-Component System Regulates Bacteroides fragilis Toxin to Maintain Intestinal Homeostasis and Prevent Lethal Disease.Cell Host Microbe. 2017 Oct 11;22(4):443-448.e5. doi: 10.1016/j.chom.2017.08.007. Epub 2017 Sep 21. Cell Host Microbe. 2017. PMID: 28943327 Free PMC article.
-
PCR Detection of the Bacteroides fragilis Enterotoxin Gene Relies on Robust Primer Design.J Clin Microbiol. 2016 Jan;54(1):239-40. doi: 10.1128/JCM.02785-15. Epub 2015 Oct 21. J Clin Microbiol. 2016. PMID: 26491187 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources