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. 2003 Jan 1;31(1):278-81.
doi: 10.1093/nar/gkg064.

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells

Doug Stryke  1 , Michiko Kawamoto, Conrad C Huang, Susan J Johns, Leslie A King, Courtney A Harper, Elaine C Meng, Roy E Lee, Alice Yee, Larry L'Italien, Pao-Tien Chuang, Stephen G Young, William C Skarnes, Patricia C Babbitt, Thomas E Ferrin
Affiliations

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells

Doug Stryke et al. Nucleic Acids Res. .

Abstract

The BayGenomics gene-trap resource (http://baygenomics.ucsf.edu) provides researchers with access to thousands of mouse embryonic stem (ES) cell lines harboring characterized insertional mutations in both known and novel genes. Each cell line contains an insertional mutation in a specific gene. The identity of the gene that has been interrupted can be determined from a DNA sequence tag. Approximately 75% of our cell lines contain insertional mutations in known mouse genes or genes that share strong sequence similarities with genes that have been identified in other organisms. These cell lines readily transmit the mutation to the germline of mice and many mutant lines of mice have already been generated from this resource. BayGenomics provides facile access to our entire database, including sequence tags for each mutant ES cell line, through the World Wide Web. Investigators can browse our resource, search for specific entries, download any portion of our database and BLAST sequences of interest against our entire set of cell line sequence tags. They can then obtain the mutant ES cell line for the purpose of generating knockout mice.

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Figures

Figure 1
Figure 1
A schematic of our automated protocol for annotating cell line sequence tags.
See this image and copyright information in PMC

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References

    1. Mural R.J., Adams,M.D., Myers,E.W., Smith,H.O., Gabor Miklos,G.L., Wides,R., Halpern,A., Li,P.W., Sutton,G.G., Nadeau,J. et al. (2002) A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome. Science, 296, 1661–1671. - PubMed
    1. Stanford W.L., Cohn,J.B. and Cordes,S.P. (2001) Gene-trap mutagenesis: past, present and beyond. Nature Rev. Genet., 2, 756–768. - PubMed
    1. Mitchell K.J., Pinson,K.I., Kelly,O.G., Brennan,J., Zupicich,J., Scherz,P., Leighton,P.A., Goodrich,L.V., Lu,X., Avery,B.J., Tate,P., Dill,K., Pangilinan,E., Wakenight,P., Tessier-Lavigne,M. and Skarnes,W.C. (2001) Functional analysis of secreted and transmembrane proteins critical to mouse development. Nature Genet., 28, 241–249. - PubMed
    1. Townley D.J., Avery,B.J., Rosen,B. and Skarnes,W.C. (1997) Rapid sequence analysis of gene trap integrations to generate a resource of insertional mutations in mice. Genome Res., 7, 293–298. - PubMed
    1. Ewing B., Hillier,L., Wendl,M.C. and Green,P. (1998) Base-calling of automated sequencer traces using Phred. I. Accuracy Assessment. Genome Res., 8, 175–185. - PubMed

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