Three-dimensional analysis of post-Golgi carrier exocytosis in epithelial cells
- PMID: 12545172
- DOI: 10.1038/ncb917
Three-dimensional analysis of post-Golgi carrier exocytosis in epithelial cells
Abstract
Targeted delivery of proteins to distinct plasma membrane domains is critical to the development and maintenance of polarity in epithelial cells. We used confocal and time-lapse total internal reflection fluorescence microscopy (TIR-FM) to study changes in localization and exocytic sites of post-Golgi transport intermediates (PGTIs) carrying GFP-tagged apical or basolateral membrane proteins during epithelial polarization. In non-polarized Madin Darby Canine Kidney (MDCK) cells, apical and basolateral PGTIs were present throughout the cytoplasm and were observed to fuse with the basal domain of the plasma membrane. During polarization, apical and basolateral PGTIs were restricted to different regions of the cytoplasm and their fusion with the basal membrane was completely abrogated. Quantitative analysis suggested that basolateral, but not apical, PGTIs fused with the lateral membrane in polarized cells, correlating with the restricted localization of Syntaxins 4 and 3 to lateral and apical membrane domains, respectively. Microtubule disruption induced Syntaxin 3 depolarization and fusion of apical PGTIs with the basal membrane, but affected neither the lateral localization of Syntaxin 4 or Sec6, nor promoted fusion of basolateral PGTIs with the basal membrane.
Similar articles
-
Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging.J Cell Biol. 2006 Mar 27;172(7):1035-44. doi: 10.1083/jcb.200512012. J Cell Biol. 2006. PMID: 16567501 Free PMC article.
-
SNARE expression and localization in renal epithelial cells suggest mechanism for variability of trafficking phenotypes.Am J Physiol Renal Physiol. 2002 Nov;283(5):F1111-22. doi: 10.1152/ajprenal.00185.2002. Am J Physiol Renal Physiol. 2002. PMID: 12372788
-
[Identification of signals and mechanisms of sorting of plasma membrane proteins in intestinal epithelial cells].J Soc Biol. 1999;193(2):131-4. J Soc Biol. 1999. PMID: 10451345 French.
-
Genesis of polarity in renal tubular cells.Miner Electrolyte Metab. 1986;12(1):20-4. Miner Electrolyte Metab. 1986. PMID: 3007959 Review.
-
Microtubule organization and function in epithelial cells.Traffic. 2004 Jan;5(1):1-9. doi: 10.1111/j.1600-0854.2003.00149.x. Traffic. 2004. PMID: 14675420 Review.
Cited by
-
AAV9-mediated SH3TC2 gene replacement therapy targeted to Schwann cells for the treatment of CMT4C.Mol Ther. 2023 Nov 1;31(11):3290-3307. doi: 10.1016/j.ymthe.2023.08.020. Epub 2023 Aug 28. Mol Ther. 2023. PMID: 37641403
-
Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE.Front Cell Dev Biol. 2022 Dec 7;10:833175. doi: 10.3389/fcell.2022.833175. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36568977 Free PMC article.
-
Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion.Research (Wash D C). 2022 Oct 16;2022:9835035. doi: 10.34133/2022/9835035. eCollection 2022. Research (Wash D C). 2022. PMID: 36340505 Free PMC article.
-
Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains.J Clin Biochem Nutr. 2022 May;70(3):222-230. doi: 10.3164/jcbn.21-141. Epub 2022 Jan 8. J Clin Biochem Nutr. 2022. PMID: 35692678 Free PMC article.
-
Transcytosis of Galectin-3 in Mouse Intestine.Methods Mol Biol. 2022;2442:367-390. doi: 10.1007/978-1-0716-2055-7_20. Methods Mol Biol. 2022. PMID: 35320536
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources